Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

Abstract

Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HAV HRP conjugate; in the second (COMP-2), test sera and anti-HAV HRP conjugate competed for HAV epitopes previously adsorbed to anti-HAV IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.

Serological markers of hepatitis A, B and C viruses in rural communities of the semiarid Brazilian Northeast

“…The seroprevalence of individuals with antibodies against HAV observed in our study (83.3%) was similar to that found in other regions of the country, where frequencies are also high [21], especially in areas with low social and economic development indexes [22].…”

Seroprevalence of viral hepatitis in riverine communities from the Western Region of the Brazilian Amazon Basin

“…All HBsAg reactive subjects were tested for antibody to HDV antigen (anti-Delta) and HBV subtyped. Anti-HAV was determined using a competitive "in-house" EIA (Vitral et al 1991). HBsAg subtyping was carried out occording the method described by Gaspar and Yoshida (1987).…”

Aspectos sociodemográficos da soroprevalência de marcadores do vírus da hepatite A no povoado de Cavunge, região do semi-árido do Estado da Bahia

“…Os anticorpos neutralizantes IgG, anti-VHA, têm títulos perceptíveis desde o início do processo infeccioso e que perduram por longo tempo, ou por toda a vida 9 . Por conta desta característica do anti-VHA IgG e pelas características associadas à transmissão do VHA, foi planejado o estudo de soroprevalência na população do Povoado de Cavunge (município de Ipecaetá, Estado da Bahia), onde também a investigação das condições sanitárias e ambientais permite avaliar possíveis inter-relações com aquela infecção viral.…”