Comparison of serology, antigenemia assay and the polymerase chain reaction for monitoring active cytomegalovirus infections in hematopoietic stem cell transplantation patients

Bonon, Sandra Helena Alves; Rossi, Claudio; Souza, Cármino Antônio de; Vigorito, Afonso Celso; Costa, Sandra Cecı́lia Botelho

doi:10.1590/s0036-46652006000500007

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…Some studies showed different levels of anti‐CMV IgG antibodies in the seropositive population, with titers of anti‐CMV IgG differing up to 50‐fold between individuals [Gargouri et al, 2000; Kanengisser‐Pines et al, 2009]. Bonon et al [2006] observed that during viral reactivation, the individuals increase anti‐CMV IgG titers; this led the researchers to conclude that, in the absence of antigen detection immunoassays, serology could be useful as a tool to monitor infections.…”

Section: Discussionmentioning

confidence: 99%

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

Khokha

Krylov

Reilly

et al. 2009

Developmental Dynamics

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Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes.

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Section: Discussionmentioning

confidence: 99%

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

Khokha

Krylov

Reilly

et al. 2009

Developmental Dynamics

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show abstract

“…Some studies showed different levels of anti-CMV IgG antibodies in the seropositive population, with titers of anti-CMV IgG differing up to 50-fold between individuals [Gargouri et al, 2000;Kanengisser-Pines et al, 2009]. Bonon et al [2006] observed that during viral reactivation, the individuals increase anti-CMV IgG titers; this led the researchers to conclude that, in the absence of antigen detection immunoassays, serology could be useful as a tool to monitor infections.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and serum profile of cytomegalovirus infection among patients with hematologic disorders in Bahia State, Brazil

Matos

Meyer

Lima

2010

Journal of Medical Virology

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Human cytomegalovirus (CMV) is a ubiquitous herpesvirus with from 30% to 100% of the general population exhibiting prior exposure by serology. This cross-sectional study evaluated the serological profile of anti-CMV antibodies and two acute-phase reaction proteins in Haematologic Disorder Patients (HDPs) from Bahia State, Brazil. Immuno-chemiluminescence assays were performed to detect anti-CMV IgM and IgG antibodies. Serological levels of High Sensitivity C-Reactive Protein (CRPH) and Alpha-1-Acid Glycoprotein (AAG) were measured using immunonephelometry. A total of 470 HDPs were enrolled, 238 (50.6%) males and 232 (49.4%) females. The overall seroprevalence of CMV was 89.4%, directly proportional to age and to the amount of blood units transfused. There was no difference between seroprevalence rates according to gender (P = 0.12). Four HDPs (0.9%) were seropositives for anti-CMV IgM, only one could be characterized as recent acute infection. The most CMV seropositive HDPs had anti-CMV IgG in low titers. There was a tendency for females to have higher anti-CMV IgG titers than men (P < 0.05). CRPH levels were different among HDPs CMV negative and positive groups (P < 0.001). There was no difference in the AAG levels between groups (P = 0.15). The high CMV seroprevalence found underscores the importance of using strategies to provide "CMV safe" blood to HDPs who are at high risk of developing severe CMV infection. CRPH can be used as a biomarker associated with CMV seropositivity; however, more efforts are needed to better characterize the clinical profile of active CMV infection in this group of patients.

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“…Thus, a hypothesis to explain the high titers of anti-CMV IgG would possibly be a recent viral reactivation, which has already been suggested by other studies. [18][19] On the other hand, it is more likely that a recent re-infection by a different CMV strain was responsible for a combination of "high titers of IgG + low avidity". However, to prove these hypotheses, more specific tests are needed, such as assessing the CMV genome in these patients.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of cytomegalovirus infection among healthy blood donors in Bahia State, Brazil

Matos¹,

Meyer²,

Lima³

2010

Rev. Bras. Hematol. Hemoter.

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We aimed at analyzing the seroprevalence of cytomegalovirus infection (CMV) and to assess particular aspects of the related immunological profile among blood donors in the State of Bahia, Brazil. Immunoassays were performed to detect anti-CMV IgG and IgM antibodies and the anti-CMV IgG avidity was evaluated. The methodology used was Enzyme Linked Immuno Sorbent Assay (ELISA) with results being confirmed by chemiluminescence. Reactivity to CMV was compared between genders and age groups. Among the 636 healthy blood donors tested, 428 (67.3%) were men and 208 (32.7%) were women. The overall seroprevalence of CMV was 87.9%; seroprevalence was statistically higher in women (94.7%) than in men (84.6%-p<0.05). No sample was positive for anti-CMV IgM antibodies. About 4.6% of the sample tested showed high titers of anti-CMV IgG; in these cases an IgG avidity assay was performed that showed: low avidity (31%), moderate avidity (21%), and high avidity (48%). The high CMV seroprevalence underscores the importance of using strategies such as leukoreduction and transfusion with CMV-seronegative blood in patients who are at high risk of developing severe CMV infection. The high titers of anti-CMV IgG antibodies and its IgG avidity profile suggest the possibility of viral reactivation or re-infection. Rev. Bras. Hematol. Hemoter.

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Comparison of serology, antigenemia assay and the polymerase chain reaction for monitoring active cytomegalovirus infections in hematopoietic stem cell transplantation patients

Cited by 8 publications

References 13 publications

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes

Seroprevalence and serum profile of cytomegalovirus infection among patients with hematologic disorders in Bahia State, Brazil

Seroprevalence of cytomegalovirus infection among healthy blood donors in Bahia State, Brazil

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