Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis

Araújo, Adriana Mello de; Barbosa, G. H. T. S.; Diniz, J. R. P.; Malagueño, Elizabeth; Azevêdo, W.M. de; Carvalho, Luiz Bezerra de

doi:10.1590/s0036-46651997000300006

Cited by 8 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our laboratories, several supports have been proposed for biomolecules immobilization: Dacron [42,43]; ferromagnetic Dacron [44,45]; polyvinyl alcohol-glutaraldehyde network [46][47][48]; polyaniline-Dacron composite [49]; plasticized filter paper with polyvinyl alcohol-glutaraldehyde [50].…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane-polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.

show abstract

Section: Resultsmentioning

confidence: 99%

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Melo-Júnior

Araújo-Filho

Lins

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

“…In our laboratories other procedures for antigen covalent immobilization on supports have been proposed such as Dacron (11), ferromagnetic Dacron (12,13), interpenetrated network of PVA-glutaraldehyde (14)(15)(16)(17), filter paper plasticized with PVA-glutaraldehyde (18), and a polyaniline-Dacron composite (19). The optimal values of F1 antigen amount, conjugate dilution and serum titer established for the present method (1.25 µg/disc; 1:4,000 and 3,200, respectively) can be compared to those found for PVA-glutaraldehyde (1.3 µg/disc; 1:6,000 and 1:12,600), plasticized filter paper (1.25 µg/disc; 1:12,000 and 1:6,400), and polyaniline-Dacron (2 µg/disc; 1:4,000 and 1:800).…”

mentioning

confidence: 99%

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

Barros

Almeida

Carvalho

et al. 2002

Braz J Med Biol Res

View full text Add to dashboard Cite

We developed an efficient method to prepare a hybrid inorganicorganic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates, with the amount of antigen being one fourth that employed in conventional PVC plates (5 µg/well). In addition to the performance of the polysiloxane/PVA-glutaraldehyde disc as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.

show abstract

“…29 Solid phase immunoassay supports have been cast from glutaraldehyde crosslinked PVOH, illustrating that antibodies can be immobilised effectively onto glutaraldehyde treated PVOH. 30 Here we investigated for the first time the sensitive quantitation of clinically relevant protein biomarkers in FEP microcapillaries with covalently immobilised capture antibodies, which involved carrying out sandwich ELISA in MCF devices with antibodies immobilised covalently using a variety of crosslinking chemistries. To produce a suitable surface for covalent antibody immobilisation to the unreactive and hydrophobic FEP microcapillaries, the inner surface of the microcapillaries was firstly coated with a layer of PVOH.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for the sensitive quantification of clinically relevant protein biomarkers

Pivetal

Pereira²,

Barbosa

et al. 2017

Analyst

View full text Add to dashboard Cite

This study reports for the first time the sensitive colorimetric and fluorescence detection of clinically relevant protein biomarkers by sandwich immunoassays using the covalent immobilisation of antibodies onto the fluoropolymer surface inside Teflon®-FEP microfluidic devices. Teflon®-FEP has outstanding optical transparency ideal for high-sensitivity colorimetric and fluorescence bioassays, however this thermoplastic is regarded as chemically inert and very hydrophobic. Covalent immobilisation can offer benefits over passive adsorption to plastic surfaces by allowing better control over antibody density, orientation and analyte binding capacity, and so we tested a range of different and novel covalent immobilisation strategies. We first functionalised the inner surface of a 10-bore, 200 μm internal diameter FEP microcapillary film with high-molecular weight polyvinyl alcohol (PVOH) without changing the outstanding optical transparency of the device delivered by the matched refractive index of FEP and water. Glutaraldehyde immobilisation was compared with the use of photoactivated linkers and NHS-ester crosslinkers for covalently immobilising capture antibodies onto PVOH. Three clinically relevant sandwich ELISAs were tested against the cytokine IL-1β, the myocardial infarct marker cardiac troponin I (cTnI), and the chronic heart failure marker brain natriuretic peptide (BNP). Overall, glutaraldehyde immobilisation was effective for BNP assays, but yielded unacceptable background for IL-1β and cTnI assays caused by direct binding of the biotinylated detection antibody to the modified PVOH surface. We found NHS-ester groups reacted with APTES-treated PVOH coated fluoropolymers. This facilitated a novel method for capture antibody immobilisation onto fluoropolymer devices using a bifunctional NHS-maleimide crosslinker. The density of covalently immobilised capture antibodies achieved using PVOH/APTES/NHS/maleimide approached levels seen with passive adsorption, and sensitive and quantitative assay performance was achieved using this method. Overall, the PVOH coating provided an excellent surface for controlled covalent antibody immobilisation onto Teflon®-FEP for performing high-sensitivity immunoassays.

show abstract

Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis

Cited by 8 publications

References 12 publications

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for the sensitive quantification of clinically relevant protein biomarkers

Contact Info

Product

Resources

About