Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories

Abstract

To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Proteccíon de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be a sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, titers of sera antibody can be rellably estimated in SMN test.

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

“…This method also provided results that correlated well with those obtained by the standard tests like MIT [10,11]. The CIET is not just qualitative but can also be used as a quantitative test to determine rabies virus or anti-rabies antibody titer [6].…”

Comparison between the Counter Immunoelectrophoresis Test and Mouse Neutralization Test for the Detection of Antibodies against Rabies Virus in Dog Sera

“…The technique was also used to determine the antigens content in lots of suckling mouse rabies and tissue culture rabies vaccines (Miceli et al 1992(Miceli et al , 1993, and to determine the antibody titer in hyperimune antirabies sera used for human prophylactic treatment (Diaz & Myers 1984). Initially developed to determine rabies antibodies in human sera (Diaz & Varela-Diaz 1977, Diaz et al 1986, Chauhan et al 1991 it was also used for equine (Diaz & Myers 1984, Diaz et al 1995, bovine (Albas et al 1995b, Oliveira et al 2000, ovine (Sanchez & Rubio 1991), and canine sera (Albas et al 1995a). The present study reports a comparison between counterimmunoelectrophore-sis test (CIET) and mouse neutralization test in the detection of antibodies against rabies virus in sera of dogs immunized along vaccination campaigns.…”