Demonstration of Viral Antibodies by the Counterimmunoelectrophoresis Test

Queiroz, Luzia Helena; Moreira, Wildeberg Cál; Moura, Wlamir Corrêa de; Silva, Marlon V.

doi:10.1016/b978-0-12-801919-1.00015-4

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…The diagnosis of AD mink takes place by looking for clinical signs and the detection of AMDV antibodies, which can be performed using non-specific methods such as iodine serum plate agglutination, where the excess of the gamma globulins levels in the serum can be visualized since certain gamma globulins can form complexes with iodine [54]. However, currently, more specific approaches are available such as counterimmunoelectrophoresis (CIEP), which involves using an electric current to assess binding resulting from the migration of unbound AMDV antigens towards the specific AMDV antibodies [55,56]. In recent times, this approach has been shown to be successful in reducing the prevalence of infected mink in Nova Scotia Canada, but has failed in eradicating AMDV from most of the infected farms [57].…”

Section: Introductionmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Viruses

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Aleutian mink disease virus (AMDV) is known to cause the most significant disease in the mink industry. It is globally widespread and manifested as a deadly plasmacytosis and hyperglobulinemia. So far, measures to control the viral spread have been limited to manual serological testing for AMDV-positive mink. Further, due to the persistent nature of this virus, attempts to eradicate Aleutian disease (AD) have largely failed. Therefore, effective strategies to control the viral spread are of crucial importance for wildlife protection. One potentially key tool in the fight against this disease is by the immunization of mink against AMDV. Throughout many years, several researchers have tried to develop AMDV vaccines and demonstrated varying degrees of protection in mink by those vaccines. Despite these attempts, there are currently no vaccines available against AMDV, allowing the continuation of the spread of Aleutian disease. Herein, we summarize previous AMDV immunization attempts in mink as well as other preventative measures with the purpose to shed light on future studies designing such a potentially crucial preventative tool against Aleutian disease.

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Section: Introductionmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Viruses

View full text Add to dashboard Cite

show abstract

“…The diagnosis of AD mink takes place by looking for clinical signs and detection of AMDV antibodies, which can be done using non-specific methods such as iodine serum plate agglutination, where excess of the gamma globulins levels in serum can be visualized since certain gamma globulins can form complexes with iodine [54]. However, currently, more specific approaches are available such as counterimmunoelectrophoresis (CIEP), which involves using an electric current to assess binding resulting from the migration of unbound AMDV antigens towards the specific AMDV antibodies [55,56]. In recent times, this approach has been shown to be successful in reducing the prevalence of infected mink in Nova Scotia Canada, but has failed in eradicating AMDV from most of the infected farms [57].…”

Section: Introductionmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Preprint

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Aleutian mink disease virus (AMDV) is known to cause the most significant disease in the mink industry. It is globally widespread and manifested as a deadly plasmacytosis and hyperglobulinemia. So far, measures to control viral spread have been limited to manual serological testing for AMDV-positive mink. Further, due to the persistent nature of this virus, attempts to eradicate Aleutian disease (AD) have largely failed. Therefore, effective strategies to control viral spread are of crucial importance for wildlife protection. One potentially key tool in the fight against this disease is by immunization of mink against AMDV. Throughout many years, several researchers have tried to develop AMDV vaccines and demonstrated varying degrees of protection in mink by those vaccines. Despite these attempts, there are currently no vaccines available against AMDV, allowing the continuation of the spread of Aleutian disease. Herein, we summarize previous AMDV immunization attempts in mink as well as other preventative measures with the purpose to shed light on future studies designing such a potentially crucial preventative tool against Aleutian disease.

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Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Остапів¹,

Kuz'mina²,

Kozak³

et al. 2021

Ukr.Biochem.J

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The counter-immunoelectrophoresis (CIe) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIe high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIe method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/ plasma antibodies of rabbits immunized against hemorrhagic disease and increased protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIe effectiveness for express diagnostic of infectious diseases. K e y w o r d s: counter-immunoelectrophoresis, amphiphilic fluorescein copolymer, rapid diagnostics, rabbit hemorrhagic disease. m e t h o d s m e t h o d s

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Demonstration of Viral Antibodies by the Counterimmunoelectrophoresis Test

Cited by 3 publications

References 13 publications

AMDV Vaccine: Challenges and Perspectives

AMDV Vaccine: Challenges and Perspectives

AMDV Vaccine: Challenges and Perspectives

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

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