Comparison between the Counter Immunoelectrophoresis Test and Mouse Neutralization Test for the Detection of Antibodies against Rabies Virus in Dog Sera

Silva, Luzia Helena Queiroz da; Bissoto, César Eduardo; Carvalho, Catherine Teixeira de; Cardoso, Tereza Cristina; Pinheiro, Devani M; Perri, Sílvia Helena Venturoli

doi:10.1590/s0074-02762002000200020

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…A high degree of specificity and reasonably adequate sensitivity, availability of basic facilities in the laboratory to perform this test, seems to favor the CIET's application as screening technique to examine large number of samples [6,9,12,31]. This method also provided results that correlated well with those obtained by the standard tests like MIT [10,11].…”

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confidence: 96%

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Kashte

Sherikar

Pingale³

2011

Indian J. Virol.

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Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller's staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller's staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.

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confidence: 96%

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Kashte

Sherikar

Pingale³

2011

Indian J. Virol.

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“…Since the doi: https://doi.org/10.15407/ubj93.01.104 antigen and antibody move in opposite directions, due to the electric field, their migration occurs towards each other. Visible precipitates appear after 30-60 minutes from the beginning of CIE [3].…”

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confidence: 99%

Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases

Остапів¹,

Kuz'mina²,

Kozak³

et al. 2021

Ukr.Biochem.J

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The counter-immunoelectrophoresis (CIe) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIe high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIe method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/ plasma antibodies of rabbits immunized against hemorrhagic disease and increased protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIe effectiveness for express diagnostic of infectious diseases. K e y w o r d s: counter-immunoelectrophoresis, amphiphilic fluorescein copolymer, rapid diagnostics, rabbit hemorrhagic disease. m e t h o d s m e t h o d s

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