Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Abstract

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs. L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively

Prevalence, Virulence and Antibiotic Susceptibility of Listeria monocytogenes Recuperated from Slaughtered Goats and Pigs of Nagpur, Central India

“…The inconsistency of hemolysin production by L. monocytogenes isolates in the present study is in agreement with the finding of Tabouret et al, (1991) and Nunes and Hofer (1994).The variation in haemolytic activity of L. monocytogenes isolates can be corroborated with the findings of Bharate (2011), Shelke (2011), Suryavanshi (2011 and Dudhe (2012) who reported the strong, moderate and weak haemolytic activity amongst the isolates of L. monocytogenes from buffaloes, cattle, goats and pigs slaughtered in the same region. This confirms the observation regarding perpetuation of variable haemolytic strains of L. monocytogenes in the same region.…”

BACTERIOCIN EXPOSURE AND FOOD INGREDIENTS INFLUENCE ON GROWTH AND VIRULENCE OF LISTERIA MONOCYTOGENES IN A MODEL MEAT GRAVY SYSTEM

“…LLO allows the pathogen to escape from the phagocytic vacuole and reach the citosol of the host cell, where it can proliferate (Dramsi and Cossart 2002). LLO is the main virulence marker for L. monocytogenes, but other laboratory tests have been proposed to complement virulence determination of this bacterium, such as the Congo red dye adsorption and production of lecithinase (McKellar 1993;Nunes and Hofer 1994). Buncic er al.…”

In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

“…The cent percent haemolytic activity showed by L. monocytogenes isolates has been reported by several researchers (Bhanu Rekha et al, 2006;Molla et al, 2004;Yucel et al, 2004;Osman et al, 2014) the same as in the present investigation and those isolates were further designated as the pathogenic one (Barbuddhe et al, 2000, Lacier andCentorbi, 2002;Chaudhari et al, 2004;Becker et al, 2006;Bhanu Rekha et al, 2006;Jallewar et al, 2007;Elezebeth et al, 2007) reveals the strong activity of LLO. Variation in haemolytic activity attributed to composition of medium, and/or a loss of ability to produce hemolysin may have occurred over successive passages through culture media which impaired the identification of these cultures as L. monocytogenes (Nunes and Hofer, 1994). CAMP test is a diagnostic tool that reliably and quickly provides presumptive identification of L. monocytogenes (Savini et al, 2014).…”