Prevalence, Virulence and Antibiotic Susceptibility of Listeria monocytogenes Recuperated from Slaughtered Goats  and Pigs of Nagpur, Central India

Vaidya, Gayatri; Chaudhary, Sunil; Zade, N. N.; Khan, Waleed Ali; Shinde, S. V.; Patil, Abhijit; Kalambhe, Deepali

doi:10.20546/ijcmas.2018.704.176

Cited by 7 publications

(8 citation statements)

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“…Antibiogram study of L. monocytogenes isolates exhibited high sensitivity against Penicillin and Cefixime and resistance against Amoxicillin and Ampicillin, which contradict with one study [41] but is consistent with another [42]. The present study showed that Ciprofloxacin, Doxycycline, and Tetracycline are intermediately sensitive, whereas [38] spot 100% sensitivity of Ciprofloxacin against L. monocytogenes.…”

Section: Discussionsupporting

confidence: 65%

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea

et al. 2021

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Background and Aim: Goat is one of the major livestock species, plays an important role in the economy of Bangladesh. However, the outbreak of different infectious diseases in goats causes high mortality and economic losses due to lack of proper diagnosis and treatment. Conventional culture-based methods for detecting specific pathogens as confirmatory diagnosis are laborious as well as time-consuming in comparison to multiplex polymerase chain reaction (mPCR), by which multiple pathogens can be detected at a time. The present study was aimed to perform faster molecular identification of bacterial pathogens from goats presumed with fever and/or diarrhea and their antimicrobial resistance (AMR) pattern. Materials and Methods: A total of 200 blood samples were collected from goats at S. A. Quaderi Teaching Veterinary Hospital (SAQTVH) in Chattogram Veterinary and Animal Sciences University for the period of July 2017-April 2018. DNA was extracted and subsequently, mPCR assay was performed for the screening of several bacterial pathogens (Salmonella spp., Listeria monocytogenes, Bacillus cereus, Yersinia enterocolitica, Campylobacter jejuni, Campylobacter coli, Clostridium perfringens, Vibrio cholerae, and Staphylococcus aureus). An antimicrobial susceptibility test against ten antimicrobials for positive samples of each organism was conducted using the Kirby–Bauer Disk-Diffusion Method on selective media. Results: S. aureus, C. perfringens, L. monocytogenes, and Salmonella spp. were detected from collected samples and their overall prevalence was 11.5%, 3.5%, 1%, and 20.5%, respectively. The most common clinical signs were mild fever, nasal discharge, dyspnea, and coughing (39.1%) for S. aureus, diarrhea, convulsion, abdominal pain, and incoordination (57.1%) for C. perfringens, fever, protrusion of tongue, and incoordination (100%) for L. monocytogenes, and fever, anorexia, dehydration with mucous feces (36.6%) for Salmonella spp. infection in goats. Antimicrobial diagram of S. aureus showed resistance against Cefotaxime (74%), Cefixime (65%), and Tetracycline (65%); highly sensitive against Amoxicillin (48%), Ciprofloxacin (44%), and Gentamicin (44%). On the other hand, C. perfringens showed highly resistant against Ampicillin (71%), Gentamicin (71%), sensitive against Penicillin (57%), and Cefotaxime (57%). L. monocytogenes were found to be sensitive to Penicillin (100%) and Cefixime (100%) and Salmonella spp. showed resistance to Ampicillin (78%) and Amoxicillin (59%) but sensitive to Ciprofloxacin (54%). Conclusion: This study identified pathogens with their specific clinical signs in goats presumed fever and/or diarrhea through mPCR with their AMR pattern in SAQTVH, Chattogram. Potential risk factors, measuring the strength of association of disease caused by these particular pathogens, were also determined. mPCR may use as an effective tool for rapid detection of pathogens in animal.

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Section: Discussionsupporting

confidence: 65%

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea

et al. 2021

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“…It is a major food-borne zoonotic bacterium of serious public health concern [ 106 , 108 , 109 ] following consumption of contaminated food of animal origin [ 110 ]. L. monocytogenes emergence as a food-borne pathogen dates from 1980, with the occurrence of many outbreaks and sporadic cases of listeriosis associated with the consumption of contaminated food [ 111 ].…”

Section: Food-borne Zoonotic Bacterial Pathogensmentioning

confidence: 99%

Section: Food-borne Zoonotic Bacterial Pathogensmentioning

confidence: 99%

“…Genus Listeria contains ubiquitous bacteria that are extensively disseminated in normal environments [106,117,118]. Listeria species are most commonly found in raw food, vegetables contaminated by soil-and water-carrying bacteria, and raw animal products [109,121]. Even though pasteurization of raw milk destroys L. monocytogenes, this process does not eliminate the later risk of contamination of dairy products [115,122].…”

Section: Listeria Monocytogenes L Monocytogenes Is a Foodborne Pathogen Widely Distributed In Naturementioning

confidence: 99%

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Review on Major Food-Borne Zoonotic Bacterial Pathogens

Abebe

Gugsa

Ahmed

2020

Journal of Tropical Medicine

316

203

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Food-borne microorganisms are major pathogens affecting food safety and cause human illness worldwide as a result of consumption of foodstuff, mainly animal products contaminated with vegetative pathogens or their toxins. Most of these microbes have zoonotic importance resulting in significant impact on both public health and economic sectors. Bacteria are the causative agents of two-thirds of human food-borne diseases worldwide with high burden in developing countries. Hence, the objectives of this review paper are to highlight the background of food-borne bacterial pathogens and to review common major food-borne zoonotic bacterial pathogens. Food animals are the major reservoirs of many food-borne zoonotic bacterial pathogens, and food products of animal origin are the main vehicles of transmission. Meat, dairy products, and eggs are the main ways by which people are exposed to zoonotic bacteria. S. aureus, Salmonella species, Campylobacter species, L. monocytogenes, and E. coli are the major zoonotic bacterial pathogens which are the causative agents of food-borne illness and death in the world associated with consumption of contaminated animal products. Production of toxins and structural virulent factors are responsible for the pathogenesis of these bacteria. These major zoonotic bacteria cause human infections which are characterized mainly by gastrointestinal symptoms including nausea, vomiting, diarrhea, abdominal cramps, and other agent-specific symptoms. Some bacteria may cause severe complications. Conventional (culturing), serological, and molecular techniques are important for detection of these common zoonotic bacteria and their toxins in food. Good hygiene, GMP, sanitation in operating procedures, and implementation of standardized HACCP and pasteurization procedures are effective methods for the control and prevention. Currently, the emergence of multidrug-resistant zoonotic bacteria associated with consumption of contaminated animal products is a great concern for the public health, and there should be coordinated surveillance and monitoring system for food-borne zoonotic bacterial pathogens particularly in developing countries including Ethiopia.

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“…Listeria monocytogenes is ubiquitous and widely distributed in nature [ 8 ] and has been isolated worldwide from humans, animals, and poultry, environmental sources such as soil, river, and decaying plants, and food sources such as milk, meat and their products, and seafood [ 4 ]. Listeria species are most commonly found in raw foods, vegetables contaminated by soil and water carrying bacteria, and in raw animal products such as meat and cheese [ 9 ]. L. monocytogenes can also be isolated from fecal samples of apparently healthy animals, poorly stored silage, and rotten vegetables.…”

Section: Introductionmentioning

confidence: 99%

Listeriosis in a peri-urban area: Cultural and molecular characterization of Listeria monocytogenes isolated from encephalitic goats

et al. 2020

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Background and Aim: Listeriosis in food animals bears a significant threat to human health. Detailed investigations into the cause facilitate proper management of the disease. This study reports the cultural, pathological, and molecular characterization of Listeria monocytogenes isolated from encephalitic goats from peri-urban Guwahati, Assam. Materials and Methods: Out of nine suspected samples, five positive isolates of L. monocytogenes were subjected to bacteriological, biochemical, and molecular tests. The genus and species-specific L. monocytogenes 16S rRNA and prs genes were amplified by polymerase chain reaction (PCR) to yield 1200 and 370 bp sized products, respectively. The encephalitic form of the disease was characterized by circling movement, high fever, and terminal recumbence. Results: All the five isolates were confirmed to be L. monocytogenes based on PCR amplification of genus and species-specific 16S rRNA and prs gene products. The isolates were sensitive to ciprofloxacin, oxytetracycline (OTC), and norfloxacin, but resistant to doxycycline and erythromycin. A high dose of OTC was used in a goat at the early stage of clinical symptom and the animal recovered clinically. Conclusion: Listeriosis in goats could pose a significant public health threat as the meat (occasionally milk) or meat products from goats are widely consumed by the people of Assam. Understanding the molecular epidemiological aspects of L. monocytogenes infections of food animal species should, therefore, be the priority in this part of the country.

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Prevalence, Virulence and Antibiotic Susceptibility of Listeria monocytogenes Recuperated from Slaughtered Goats and Pigs of Nagpur, Central India

Cited by 7 publications

References 14 publications

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea

Review on Major Food-Borne Zoonotic Bacterial Pathogens

Listeriosis in a peri-urban area: Cultural and molecular characterization of Listeria monocytogenes isolated from encephalitic goats

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