In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

Dudhe, Nitin Chandhrakant

doi:10.14737/journal.aavs/2014/2.6.321.328

Adv. Anim. Vet. Sci.

2014

DOI: 10.14737/journal.aavs/2014/2.6.321.328

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In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

Nitin Chandhrakant Dudhe¹

Abstract: Listeria monocytogenes is one of the most virulent foodborne zoonotic pathogen with 20 to 30% of clinical infections resulting in death. The L. monocytogenes is ubiquitous in the nature and has been isolated from a variety of foods and environmental sources infecting human and animals revealing the pathogenicity of organism. Reports are very few regarding the pathogenesis of organism in Nagpur region, Maharashtra, India. So the present study was proposed to evaluate the pathogenicity of 12 L.monocytogenes isol… Show more

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Cited by 2 publications

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The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

2017

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Listeria monocytogenes are Gram-positive well-known emerging food-borne pathogens causing listeriosis in humans. In the present study, we have isolated biofilm-forming Listeria sp. from utensils used by a local milk collection dairy society at Usgao Goa, which collects milk for Goa dairy. Through biochemical tests and 16S rRNA sequence analysis, the bacterium was confirmed to be L. monocytogenes and designated as strain BN3, having GenBank accession number MF095110. We report for the first time Gram-positive L. monocytogenes strain BN3 producing iron-chelating siderophores by chrome azurol S (CAS) agar test. Also, this is a first report which reveals that L. monocytogenes strain BN3 responds to N-hexanoyl-homoserine lactone molecule (C-HSL) by gradual increase in their biofilm-forming potential with a gradual increase in AHL (C-HSL) concentration (250, 500 nM-1 μM) as compared to control revealed by crystal violet assay (CV) in microtiter plate. These results were further confirmed by scanning electron microscopy (SEM). A significant decrease in biofilm formation was observed when L. monocytogenes strain BN3 was treated with 10 µg/ml (R)-2-(2-hydroxynaphthalen-1-yl)thiazolidine-4-carboxylic acid, but when 250 and 500 nM AHL molecules were added, biofilm formation in strain BN3 was found to be enhanced as compared to control even in the presence of antibacterial compound, (R)-2-(2-hydroxynaphthalen-1-yl)thiazolidine-4-carboxylic acid. These results revealed that AHL molecules nullify the effect of antimicrobial compound and promote biofilm formation in L. monocytogenes strain BN3.

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The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

2017

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Prevalence, Virulence Potential, and Antibiotic Susceptibility Profile ofListeria monocytogenesIsolated From Bovine Raw Milk Samples Obtained From Rajasthan, India

SharmaSanjita¹,

SharmaVishnu²,

Kumar³

et al. 2017

Foodborne Pathogens and Disease

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Listeriosis is a serious foodborne disease of a global concern, and can effectively be controlled by a continuous surveillance of the virulent and multidrug-resistant strains of Listeria monocytogenes. This study was planned to investigate prevalence of L. monocytogenes in bovine raw milk samples. A total of 457 raw milk samples collected from 15 major cities in Rajasthan, India, were analyzed for the presence of L. monocytogenes by using standard microbiological and molecular methods. Five of the 457 samples screen tested positive for L. monocytogenes. Multiplex serotyping showed that 3/5 strains belonged to serotype 4b followed by one strain each to 1/2a and to 1/2c. Further virulence potential assessment indicated that all strains possessed inlA and inlC internalins, and, in addition, two strains also possessed the gene for inlB. All strains were positive for Listeriolysin O (LLO) and showed phosphatidylinositol-specific phospholipase C (PI-PLC) activity on an in vitro agar medium with variations in production levels among the strains. A good correlation between the in vitro pathogenicity test and the chick embryo test was observed, as the strains showing higher LLO and PI-PLC activity were found to be lethal to fertilized chick embryos. All strains were resistant to the majority of antibiotics and were designated as multidrug-resistant strains. However, these strains were susceptible to 9 of the 22 tested antibiotics. The maximum zone of inhibition (mm) and acceptable minimum inhibitory concentration were observed with azithromycin, and thus it could be the first choice of a treatment. Overall, the presence of multidrug-resistant L. monocytogenes strains in the raw milk of Rajasthan region is an indicator of public health hazard and highlighting the need of consumer awareness in place and implementation of stricter food safety regulations at all levels of milk production.

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In-Vitro Characterization Of Listeria Monocytogenes Isolates By Haemolysis, Camp, Piplc Assay With Protein Profiling And Antibiotic Resistance Recovered From Nagpur Region

Cited by 2 publications

References 30 publications

The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

Prevalence, Virulence Potential, and Antibiotic Susceptibility Profile ofListeria monocytogenesIsolated From Bovine Raw Milk Samples Obtained From Rajasthan, India

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