The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

Naik, Milind Mohan; Bhangui, Purva; Bhat, Chinmay

doi:10.1007/s00203-017-1416-8

Cited by 10 publications

(19 citation statements)

References 25 publications

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“…QS is a cell-to-cell communication used by many bacteria to coordinate common behaviors and regulate a diverse array of physiological activities through the sensing of cell-population densities. In this process, bacteria produce, release, and detect chemical signal molecules called 'autoinducers' (AI) that increase in concentration concomitantly with cell density [32][33][34]. The reach of a minimal threshold stimulatory concentration leads to activation or induction of QS-dependent gene expression among a responding bacterial population.…”

Section: Quorum Sensing and Its Entanglement With The Expression Of Vmentioning

confidence: 99%

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

et al. 2020

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The worldwide emergence of antibiotic-resistant bacteria and the thread of widespread superbug infections have led researchers to constantly look for novel effective antimicrobial agents. Within the past two decades, there has been an increase in studies attempting to discover molecules with innovative properties against pathogenic bacteria, notably by disrupting mechanisms of bacterial virulence and/or biofilm formation which are both regulated by the cell-to-cell communication mechanism called ‘quorum sensing’ (QS). Certainly, targeting the virulence of bacteria and their capacity to form biofilms, without affecting their viability, may contribute to reduce their pathogenicity, allowing sufficient time for an immune response to infection and a reduction in the use of antibiotics. African plants, through their huge biodiversity, present a considerable reservoir of secondary metabolites with a very broad spectrum of biological activities, a potential source of natural products targeting such non-microbicidal mechanisms. The present paper aims to provide an overview on two main aspects: (i) succinct presentation of bacterial virulence and biofilm formation as well as their entanglement through QS mechanisms and (ii) detailed reports on African plant extracts and isolated compounds with antivirulence properties against particular pathogenic bacteria.

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Section: Quorum Sensing and Its Entanglement With The Expression Of Vmentioning

confidence: 99%

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

et al. 2020

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“…In particular, L. monocytogenes AIP is encoded by the agrBDCA operon and directly affects its adherence [13] and cell invasion capacity [14]. Nevertheless, a recent study carried out by Naik, Bhangui and Bhat demonstrates that despite the lack of production of HSL-mediated system molecules, L. monocytogenes is able to positively respond to short-chain homoserine lactones (i.e., C 6 -HSL), promoting its biofilm-forming capacity on polystyrene plates and borosilicate glass coverslips [15].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

Rodríguez-López

Barrenengoa

Pascual-Sáez

et al. 2019

Foods

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Furanones are analogues of acylated homoserine lactones with proven antifouling activity in both Gram-positive and Gram-negative bacteria though the interference of various quorum sensing pathways. In an attempt to find new strategies to prevent and control Listeria monocytogenes biofilm formation on stainless steel (SS) surfaces, different concentrations of six synthetic furanones were applied on biofilms formed by strains isolated from food, environmental, and clinical sources grown onto AISI 316 SS coupons. Among the furanones tested, (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone and 3,4-Dichloro-2(5H)-furanone significantly (p < 0.05) reduced the adhesion capacity (>1 log CFU cm−2) in 24 h treated biofilms. Moreover, individually conducted experiments demonstrated that (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone was able to not only significantly (p < 0.05) prevent L. monocytogenes adhesion but also to reduce the growth rate of planktonic cells up to 48 h in a dose-dependent manner. LIVE/DEAD staining followed by epifluorescence microscopy visualisation confirmed these results show an alteration of the structure of the biofilm in furanone-treated samples. Additionally, it was demonstrated that 20 µmol L−1 of 3,4-Dichloro-2(5H)-furanone dosed at 0, 24 and 96 h was able to maintain a lower level of adhered cells (>1 log CFU cm−2; p < 0.05). Since furanones do not pose a selective pressure on bacteria, these results represent an appealing novel strategy for the prevention of L. monocytogenes biofilm grown onto SS.

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“…Quorum sensing (QS) is a cell to cell communication mechanism between bacteria which helps in regulating biofilm development, plasmid transfer, swarming, bioluminescence etc. (Irie and Parsek 2008;LaSarre and Federle 2013;Naik et al 2017). It also helps in provoking the expression of virulence genes like exotoxin, siderophores, enzymes, adhesion molecules (Novick and Muir 1999;Gonzalez and Keshavan 2006;LaSarre and Federle 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Both Gram positive and Gram-negative bacteria possess different types of quorum sensing systems (QS). Gram positive bacteria use peptides based QS and Gram-negative bacteria use acyl homoserine lactone (AHL) molecules based QS (LaSarre and Federle 2013; Naik et al 2017). There is only one report demonstrating AHL as quorum sensing system in a marine Gram-positive bacteria Exiguobacterium sp.…”

Section: Introductionmentioning

confidence: 99%

“…(Biswa and Doble 2013), but till date there is no report on production of AHL by Staphylococcus spp. There is report saying that Staphylococcus aureus responds negatively to exogenous AHL by down regulation of virulence genes such as exotoxin, cell wall fibronectin binding proteins and agr QS system (Qazi et al 2006), whereas Listeria monocytogenes strain BN3 reported to respond positively to AHL molecules by increased biofilm development (Naik et al 2017). But till date there is no report on response of Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced exopolysaccharide production and biofilm forming ability in methicillin resistant Staphylococcus sciuri isolated from dairy in response to acyl homoserine lactone (AHL)

Naik

Dubey

et al. 2018

J Food Sci Technol

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is an emerging human pathogen widely found in dairy industries. In this study, we have isolated methicillin resistant sp. from biofilm formed on utensil used in the dairy society situated at Raia, Goa and was designated as NN14. The isolate NN14 was identified through 16S rRNA sequencing as (GenBank accession number MF621976). This report reveals that the strain NN14 responds positively to the, acyl-homoserine lactone (AHL) having 6-carbon long acyl chain i.e.-hexanoyl-homoserine lactone molecule (C-HSL) with gradual rise in their biofilm establishing potential as the concentration of AHL was increased from 250 nM, 500 nM to 1 µM when compared to control (without C6-HSL) by performing crystal violet assay using 48 well microtiter plate. Also, exopolysaccharide (EPS) production was found to increase with gradual increase in C-HSL concentration from 250 nM, 500 nM to 1 µM proving potential role of EPS in biofilm formation. These results were further proved by scanning electron microscopy where increased in biofilm and EPS production with increase in C-HSL concentration was observed. The biofilm forming capability of strain NN14 was found to decreased significantly when it was subjected to 10 µg/ml of ()-2-(2-hydroxynaphthalen-1-yl)-thiazolidine-4-carboxylic acid, however with the addition of 250 and 500 nM, C6-HSL in presence of the antimicrobial compound ()-2-(2-hydroxynaphthalen-1-yl)-thiazolidine-4-carboxylic acid, the biofilm development in bacterial strain NN14 was increased when compared with control. Our results demonstrated that the C6-HSL molecule neutralize the effect of antibacterial compound and enhances EPS production and biofilm development in .

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The first report on Listeria monocytogenes producing siderophores and responds positively to N-acyl homoserine lactone (AHL) molecules by enhanced biofilm formation

Cited by 10 publications

References 25 publications

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

Efficacy of Synthetic Furanones on Listeria monocytogenes Biofilm Formation

Enhanced exopolysaccharide production and biofilm forming ability in methicillin resistant Staphylococcus sciuri isolated from dairy in response to acyl homoserine lactone (AHL)

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