Entamoeba histolytica zymodemes in children of Osasco, São Paulo

Silva, Ivanize da; França, Expedito; Nozaki, Tomoyoshi; Freitas, Giselia Barros; Tateno, Seiki

doi:10.1590/s0036-46651993000600016

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…The methodology used in these studies has ranged from traditional serologic tests, such as gel diffusion precipitin (GDP) and zymodemes, to molecular biology techniques such as restriction endonuclease digestion of amplified genomic DNA. [3][4][5][6][7] These investigations showed the presence of both E. histolytica and E. dispar in the Amazon region (northern area) with a higher prevalence of E. histolytica, while E. dispar predominated in the northeastern region.…”

Section: Introductionmentioning

confidence: 81%

Determination of the Prevalence of Entamoeba Histolytica and E. Dispar in the Pernambuco State of Northeastern Brazil by a Polymerase Chain Reaction

Pinheiro¹,

Carneiro²,

Ivanise³

et al. 2004

The American Journal of Tropical Medicine and Hygiene

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Previous studies using methods varying from traditional serologic tests to molecular biology techniques have shown that in northeastern Brazil, Entamoeba dispar was more prevalent than E. histolytica. In this study, the prevalence was established by using E. histolytica stool antigen detection kits and a polymerase chain reaction (PCR) with genomic DNA extracted from cultured trophozoites in all four-nuclei, amoeba-positive samples from a population living in Macaparana in northeastern Brazil. Among 1,437 stool samples analyzed, only 59 (4.1%) were positive for four nuclei amoeba. However, all of these samples were negative in an immunoenzymatic assay for the presence of E. histolytica-specific galactose adhesin. Of 59 cultivated samples, only 31 showed trophozoites. Extraction of DNA from these 31 samples, followed by the PCR, showed that 23 samples (74.19%) were positive for E. dispar and no amplification was observed for pathogenic E. histolytica. The remaining eight samples were negative for both species. These findings are consistent with those previously reported.

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Section: Introductionmentioning

confidence: 81%

Determination of the Prevalence of Entamoeba Histolytica and E. Dispar in the Pernambuco State of Northeastern Brazil by a Polymerase Chain Reaction

Pinheiro¹,

Carneiro²,

Ivanise³

et al. 2004

The American Journal of Tropical Medicine and Hygiene

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“…4). The species identified as non-pathogenic E. histolytica, through zymodeme [2,3], were considered as E. dispar. On the other hand, E. coli was the only species with a taxonomic classification, identified in animal hosts.…”

Section: Entamoeba Spp Diversity In Different Host Species In Brazilmentioning

confidence: 99%

Diversity, geographical distribution, and prevalence of Entamoeba spp. in Brazil: a systematic review and meta-analysis

Zanetti¹,

Malheiros²,

Matos³

et al. 2021

Parasite

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The genus Entamoeba includes a variety of widely distributed species adapted to live in the digestive tracts of humans and a large variety of animals of different classes. The objective of this study was to investigate the prevalence, distribution, and molecular epidemiology of Entamoeba spp. in different classes of hosts in Brazil. Studies that analyzed hosts from several classes, including humans and domestic, wild, or captive animals, were considered. The pooled prevalence of Entamoeba spp. was calculated using the random-effects model. A total of 166 studies on humans and 16 on animals were included. The prevalence of Entamoeba spp. in the Brazilian population was 22% (95% CI: 21–24). The state with the highest prevalence was Paraiba with 72%, followed by Federal District with 53%, and Rondonia with 50%. In immunocompromized patients, the prevalence was 18%, and cancer (36%) was the most prevalent cause of immunosuppression. The prevalence of Entamoeba spp. in animal hosts was 12% (95% CI: 7–17). Captive wild animals and domestic farm animals showed the highest prevalence, with 16% and 15%, respectively. The species found more often were E. coli (86.5%), E. dispar (7.9%), and E. histolytica (3.1%). In conclusion, a high prevalence (22%) of Entamoeba spp. was found in the Brazilian population, with a prevalence of up to 50% mainly in the northern, northeastern, and central-western regions. The pathogenic species E. histolytica is distributed in most Brazilian regions, with significant prevalence percentages. Among animals, unidentified Entamoeba species were most prevalent in mammals.

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