Genetic characterization of Entamoeba dispar isolates in Northeast Brazil

Pinheiro, Sandra Maria Botelho; Maciel, Rogério F.; Morais, Marcos Antônio de; Ivanize, S.; Carvalho, Luiz Bezerra de; Coimbra, Maria Raquel Moura

doi:10.1016/j.actatropica.2005.01.012

Cited by 9 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microsatellite typing is performed by amplifying the microsatellite by PCR using specific primers. Two minisatellite loci containing internal repeats, loci 1-2 and 5-6, have demonstrated variable polymorphism for E. histolytica and E. dispar (136,211,212), indicating that these loci have the potential to be used as molecular markers for investigating the epidemiology of the two Entamoeba species.…”

Section: Typing Methodsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

View full text Add to dashboard Cite

SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

show abstract

Section: Typing Methodsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

View full text Add to dashboard Cite

show abstract

“…These studies have identified genetic variation in protein-coding sequences of E. histolytica , such as those for the serine-rich E. histolytica protein [ 10 - 15 ] and chitinase [ 8 , 11 , 12 ], as well as non-protein-coding regions such as the ribosomal RNA (rRNA) genes [ 4 , 5 , 7 ] and loci 1–2 and 5–6 [ 11 , 12 , 16 , 17 ]. In addition, the existence of genetic variation in non-protein-coding loci 1–2 and 5–6 [ 18 ], as well as protein-coding chitinase gene of E. dispar has been reported recently [ 19 ].…”

Section: Introductionmentioning

confidence: 99%