High genetic diversity among Iranian Entamoeba dispar isolates based on the noncoding short tandem repeat locus D-A

Nazemalhosseini‐Mojarad, Ehsan; Haghighi, Ali; Kazemi, Bahram; Rostami‐Nejad, Mohammad; Abadi, Ashraf H.; Zali, Mohammad Reza

doi:10.1016/j.actatropica.2009.03.008

Cited by 8 publications

(3 citation statements)

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“…Although we have to mention that in the case of E. histolytica and E. dispar species, there is not an antecedent of the genetic diversity measured by the segregating sites and the rates of π and θ, values of genetic diversity observed in the two groups constructed with E. dispar sequences can be considered high, in accordance with previous reports where the number of haplotypes was estimated [10,11,25] (Table 2). In the group that included only liver abscess samples analyzed in this study, using four sequences we obtained three haplotypes, 33 segregating sites and values of π and θ of 0.065 and 0.071, respectively.…”

Section: Resultssupporting

confidence: 89%

Human Amebiasis: Breaking the Paradigm?

Ximénez

Cerritos

Rojas

et al. 2010

IJERPH

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For over 30 years it has been established that the Entamoeba histolytica protozoan included two biologically and genetically different species, one with a pathogenic phenotype called E. histolytica and the other with a non-pathogenic phenotype called Entamoeba dispar. Both of these amoebae species can infect humans. E. histolytica has been considered as a potential pathogen that can cause serious damage to the large intestine (colitis, dysentery) and other extraintestinal organs, mainly the liver (amebic liver abscess), whereas E. dispar is a species that interacts with humans in a commensal relationship, causing no symptoms or any tissue damage. This paradigm, however, should be reconsidered or re-evaluated. In the present work, we report the detection and genotyping of E. dispar sequences of DNA obtained from patients with amebic liver abscesses, including the genotyping of an isolate obtained from a Brazilian patient with a clinical diagnosis of intestinal amebiasis that was previously characterized as an E. dispar species. The genetic diversity and phylogenetic analysis performed by our group has shown the existence of several different genotypes of E. dispar that can be associated to, or be potentiality responsible for intestinal or liver tissue damage, similar to that observed with E. histolytica.

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Section: Resultssupporting

confidence: 89%

Human Amebiasis: Breaking the Paradigm?

Ximénez

Cerritos

Rojas

et al. 2010

IJERPH

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“…Given these facts, one would expect a higher frequency of E. histolytica , and greater genetic diversity of E. histolytica . However, it seems to be a common phenomenon, which has been observed in other endemic communities, that the same genotypes circulate from one individual to another within the community; in our study, the transmission patterns of the parasites went from the homes to the school and viceversa [ 5 , 12 , 13 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 60%

“…Even though there is not enough information about the genetic diversity estimated through the π and θ rates, the previous values are higher than those obtained in the present work [ 14 ]. Escueta-de Cadiz et al (2010) [ 39 ] reported the genotyping of E. histolytica using the marker NK2H3-H5; they identified 5 motifs with 8 nucleotides each, which indicates that E. histolytica is less polymorphic than E. dispar ; the use of the D-A genetic marker [ 13 ] demonstrated that E. histolytica is indeed less polymorphic than E. dispar . The low genetic diversity of our isolates suggests that most of the segregative sites are found in only a few sequences.…”

Section: Discussionmentioning

confidence: 99%

Entamoeba histolytica and Entamoeba dispar infection in Mexican school children: genotyping and phylogenetic relationship

et al. 2016

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BackgroundThis study aimed to determine the frequency of Entamoeba histolytica and Entamoeba dispar infection in school children in the community of Tlaltizapan, in order to understand the dynamics of infection within the school and family spheres of this population. Amoebiasis is an unsolved public health problem and an endemic disease in Mexico. The incidence rate varies depending on the state; the most affected states show the highest numbers of new cases of amoebiasis per year. Previously, we reported the molecular frequency of infection with E. histolytica and/or E. dispar in other rural communities of the state of Morelos.MethodsChildren from 3 schools were studied to estimate the frequency of intestinal parasites through microscopic examination of fresh stool samples. The number of studied individuals were 309 school children. The molecular characterization of E. histolytica or E. dispar was carried out by Polymerase Chain Reaction (PCR) using species-specific primers to amplify short tandem repeats (STR) in non-coding sequences associated with the tRNA gene; the amplified fragments were sequenced and analyzed.ResultsEight different genotypes were obtained from E. dispar isolates with the molecular marker NKD3-D5. None of the cases in which the species E. histolytica was detected developed symptoms attributable to an invasive process of disease. Moreover, the parasitized condition appeared to have no significant impact on the development or nutritional status of affected children. Genotype 1, which corresponds to the reference strain E. dispar SAW760, considered a non-pathogenic amoeba, was the most prevalent.ConclusionsThe comparison of the genotypes of Entamoeba species did not show a correlation between children and their relatives. In this community, the species Entamoeba dispar genotype 1 was the most widespread. Based on the indicators of growth, development and nutrition status, the studied community seems to be reasonably adapted to constant exposure to intestinal parasites, since there were no evidences of a serious impact of the parasitized condition on the children’s health.

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The Genomics of Entamoebae: Insights and Challenges

Weedall

2014

Amebiasis

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High genetic diversity among Iranian Entamoeba dispar isolates based on the noncoding short tandem repeat locus D-A

Cited by 8 publications

References 21 publications

Human Amebiasis: Breaking the Paradigm?

Human Amebiasis: Breaking the Paradigm?

Entamoeba histolytica and Entamoeba dispar infection in Mexican school children: genotyping and phylogenetic relationship

The Genomics of Entamoebae: Insights and Challenges

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