Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil

Takimoto, Sueko; Tanaka, Harki; Gomes, Luis F. Salles; Waldman, Eliseu Alves; Perreira, Helio G.; Paiva, T. M. N.; Coimbra, Teresinha L.; Ueda, Marli; Meles, Heloísa H. G.

doi:10.1590/s0036-46651990000300003

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…The remaining cases were negative. More recently, TAKIMOTO et al 15 reported a study including 138 AM patients with the ages ranging from 4 months to 57 years, attending several hospitals in São Paulo State. A probable new agent was isolated from the cerebrospinal fluid of 35 of 53 specimens examined.…”

Section: Introductionmentioning

confidence: 99%

Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil

Gomes

Kopecká

Linhares

2001

Rev. Inst. Med. trop. S. Paulo

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Eighty-one cerebrospinal fluid (CSF) samples mainly from cases of aseptic meningitis and motor deficiency syndrome were sent to the Virology Section of Evandro Chagas Institute, Belém Pará, in the period of January 1995 to January 1996 in order to isolate viruses. All samples were inoculated onto HEp-2 cell culture and newborn mice, with negative results. The probability of isolating viruses by these methods is reduced because of the low concentration of viral particles in these specimens. In order to obtain more information about the etiology of these cases, a group of 23 samples were selected to be tested by a more sensitive technique than the virus isolation - the reverse transcription polymerase chain reaction (RT-PCR). Specific primers directed to conserved regions in the enterovirus genome were used, considering that this group of viruses is frequently associated with these neurological disorder. The age of the patients ranged from 1 to 55 years and nearly all of them lived in Belém, State of Pará, North of Brazil. Of 15 samples analyzed by RT PCR nine (60%) were positive; of these, 6 (66.6%) had motor deficiency and 3 (33.3%) developed aseptic meningitis. These results show that it is important to investigate enterovirus as cause of these syndromes.

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Section: Introductionmentioning

confidence: 99%

Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil

Gomes

Kopecká

Linhares

2001

Rev. Inst. Med. trop. S. Paulo

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Síndrome da meningite asséptica por enterovírus e Leptospira sp em crianças de Salvador, Bahia

Silva

Tanajura

Tavares-Neto

et al. 2002

Rev. Soc. Bras. Med. Trop.

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For the purpose of identifying the frequency that enterovirus, leptospires, arbovirus cause aseptic meningitis syndrome (AMS) during non-epidemic periods and comparing patients with and without laboratory evidence for an etiologic agent, 112 patients were selected aged between 3 months and 15 years and a clinical suspicion of AMS and were referred to Couto Maia Hospital, the Infectious and Parasitic Disease Reference Center for Salvador, Bahia. In 44.6% (n=50), the etiologic agent for the diagnosis was laboratory-confirmed: enterovirus was identified in 37.7% (n=42) of the cases by the PCR Amplicor diagnostic kit, cerebrospinal fluid or fecal culture isolation; Leptospira sp. in 7.12% (n=8) by the microagglutination test; and arbovirus in non of the cases by inhibition of passive hemagglutination. In 14 of the 22 enteroviral isolates that were evaluated, 6 different serotypes were identified with Echovirus-4 being the major serotype (27.2%; 6/22) among all found (Coxsackie B2, B3, B6 and B9; Enterovirus 71). In conclusion, enteroviruses were the most frequent etiologic agent of AMS and that leptospirosis should be included in the differential diagnosis. In addition, patients with and without laboratory-confirmed identification of the etiologic agent had similar demographic and clinical characteristics and cerebrospinal fluid findings (p >0.05), therefore suggesting that patients without a confirmed diagnosis had enteroviral or leptospiral etiologies.

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Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil

Cited by 2 publications

References 10 publications

Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil

Detection of enteroviruses in cases of neurological disorders in the State of Pará, Brazil

Síndrome da meningite asséptica por enterovírus e Leptospira sp em crianças de Salvador, Bahia

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