Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol

Camargo, Edwaldo E.; Sato, Mariana Eri; Negro, G. Del; Lacaz, Carlos da Silva

doi:10.1590/s0036-46651987000500005

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Assim como outros autores 25 , observou-se aqui marcante predominância do sexo masculino (p <0,001). É pro v á v e l que isto se deva à proteção fornecida pelos hormô-nios femininos, uma vez que os estrogênios inibem o crescimento do fungo in vitro 9 . Na forma granulomatosa, o diagnóstico diferencial deve incluir: toxoplasmose, neoplasias, particularmente, gliomas malignos e tumores metastáticos, tuberculose, abscessos bacterianos, neuro c i s t i c e rcose, além das outras micoses como criptococose e histoplasmose 4 , 2 3 , 2 5 , 3 9 .…”

Section: Tabela Avaliação De Treze Pacientes Com Pcm No Snc Após Trunclassified

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Fagundes-Pereyra

Carvalho²,

Góes

et al. 2006

Arq. Neuro-Psiquiatr.

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A paracoccidioidomicose (PCM) é infecção granulomatosa sistêmica, causada pelo fungo Paracoccidioides brasiliensis, prevalente na América Latina, particularmente no Brasil. Acomete o sistema nervoso central (SNC) em 10% dos casos. Foram estudados 13 pacientes com paracoccidioidomicose no SNC, entre 1991 e 2001, com ênfase para os aspectos clínicos, neuroradiológicos e terapêuticos. Onze pacientes eram do sexo masculino (84,6%) e dois do feminino (15,4%), com idade entre 30 e 71 anos (M= 47,1 ± 11,6 Me= 46). Os sintomas mais freqüentes foram déficits motores (53,8%), alterações cognitivas (53,8%), emagrecimento (46,1%), cefaléia (46,1%) e crises convulsivas (46,1%). O diagnóstico foi confirmado pela detecção do P. brasiliensis no SNC. Todos os pacientes apresentavam a forma granulomatosa e quatro (30,8%) tinham a forma meningoencefalítica associada. Todos foram estudados com tomografia computadorizada (TC) de crânio e um caso com ressonância magnética (RM) encefálica. Dez pacientes (76,9%) realizaram sorologia para o HIV, todos com resultados negativos. A anfotericina B foi utilizada em 12 casos (92,3%), em um deles por via intratecal. Em oito casos (61,5%) o sulfametoxazol-trimetropim foi utilizado; em dois (15,4%) a sulfadiazina e pirimetamina, e o fluconazol, cetoconazol e itraconazol, cada um deles em um paciente. Seis pacientes (46,1%) morreram e sete evoluíram satisfatoriamente. O tempo de seguimento variou de 2 a 74 meses (M=30,9). Conclui-se que as manifestações clínicas assim como os exames de imagem na PCM do SNC são inespecíficos.

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Section: Tabela Avaliação De Treze Pacientes Com Pcm No Snc Após Trunclassified

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Fagundes-Pereyra

Carvalho²,

Góes

et al. 2006

Arq. Neuro-Psiquiatr.

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“…A radiometric technique was used to promptly measure P. brasiliensis growth and metabolic activity so that the effects of cert in compounds on fungal development could be determi ed more rapidly (44). For instance, it was shown that the ddition of amphotericin B (105 ,ug/ml) and diethylstilbestr l (5 ,ug/ml) inhibited the normal metabolism of the fungus.…”

Section: Etiologic Agentmentioning

confidence: 99%

Paracoccidioidomycosis: an update

1993

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This review summarizes knowledge on various aspects of paracoccidioidomycosis. Mycelial propagules, chlamydospores, and arthroconidia exhibit thermal dimorphism; arthroconidia are infectious in animals and, by electron microscopy, appear well provided for survival. The mycelial-to-yeast-phase transformation requires a strict control of glucan synthesis probably mediated by membrane enzymes. Hormonal influences on the transformation of the fungus (mycelium or conidium to yeast phase) have been demonstrated. Estrogen-binding proteins have been detected in the fungal cytosol, and during the transformation novel proteins are produced as a result of estradiol incorporation. Clinical forms have been better defined on the basis of better experimental models. Emphasis has been placed on the lungs as the portal of entry and on the existence of silent pulmonary infections. A specific Paracoccidioides brasiliensis antigen, the 43-kDa glycoprotein (Gp43), has been identified, characterized, and cloned. This has led to improved reproducibility and specificity of serologic tests. The depression of cell-mediated immune responses has been associated with severe disease in humans and in the experimental host. T-cell subsets in patients' tissues were characterized by means of monoclonal antibodies, and a reduced CD4/CD8 ratio was demonstrated. This has been related to alterations in lymphokine and tumor necrosis factor production, production of antigen-antibody complexes, etc. Amphotericin B has provided effective therapy. Azole derivatives have also improved prognosis and facilitated therapy. Itraconazole is presently the drug of choice, yet incapacitating sequelae (mainly pulmonary fibrosis) still constitute major problems.

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In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms

Shikanai‐Yasuda

Taguchi

Sato³

et al. 1991

Rev. Inst. Med. trop. S. Paulo

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The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media.

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Radiometric detection of metabolic activity of Paracoccidioides brasiliensis and its susceptibility to amphotericin B and diethylstilbestrol

Cited by 3 publications

References 7 publications

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Paracoccidioidomicose do sistema nervoso central: análise de 13 casos

Paracoccidioidomycosis: an update

In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms

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