Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Barbosa, Letícia Mascarenhas Pereira; Neto, Vespasiano Borges de Paiva; Dias, Leonardo Lucas Carnevalli; Festucci-Buselli, Reginaldo Alves; Alexandre, Rodrigo Sobreira; Iarema, Lourdes; Finger, Fernando Luíz; Otoni, Wagner Campos

doi:10.1590/s0034-737x2013000200002

Cited by 11 publications

(13 citation statements)

References 18 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, analyzing its anatomy becomes a valuable instrument to predict leaf survival at this stage (Jausoro et al 2010). The deformation of epidermal cells in hyperhydric leaves negatively affects structures such as stomata (Barbosa et al 2013) to mesophilic cell disorganization, fact that may compromise plants' vital physiological processes such as photosynthesis (Palma et al 2011). In addition, the stomatal mechanism inefficiency may lead to tissue dehydration and plant death during the acclimatization stage (Palma et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Controlling hyperhydricity in micropropagated plants of Lippia grata Schauer (Verbenaceae), a native species of a dry seasonal tropical forest with pharmacological potential

et al. 2018

View full text Add to dashboard Cite

The present study is to assess the morphophysiological development in vitro of Lippia grata Schauer, an endemic ''Caatinga'' species that shows pharmacological properties. Young cuttings containing nodal segments were divided into three explant types, namely E1 (apical bud), E2 (nodal segment below the apical bud) and E3 (nodal segment below E2); the explants were inoculated in MS medium supplemented with antioxidant compound (polyvinylpyrrolidone) or adsorbing agent (activated charcoal). The survival index, the occurrence of oxidation, contamination and the number of leaves and sprouts were assessed. The explants were cultivated in MS medium supplemented with polyethylene glycol 6000 (0, 0.5 and 1 g L-1) and closed with aluminum foil (total sealing) or with aluminum foil containing one hole (partial sealing), in the multiplication stage, due to hyperhydricity in vitro. Histological and biometric analyses were performed at the 45th cultivation day, as well as the analyses of antioxidative enzyme, and hydrogen peroxide and malondialdehyde levels. Normal leaves have presented greater organization and cellular development than hyperhydric leaves. The use of antioxidants was not efficient to control the oxidation of inoculated apical buds (E1), only. The lowest hyperhydricity rates in the multiplication stage were found in the partial sealing treatments; however, the total sealing of tubes, in combination with the PEG addition, has led to increased lipid peroxidation. In view of the results, it is concluded that the explant types, as well as the suitability of a functional gas exchange system in vitro, have favored the growth and development of cells and tissues, thus attenuating the hyperhydricity symptoms. In addition, this study demonstrates the suitability of the culture system through the facilitation of gas exchange allows the use of the protocol for in vitro propagation without compromising the leaf morphoanatomy.

show abstract

Section: Discussionmentioning

confidence: 99%

Controlling hyperhydricity in micropropagated plants of Lippia grata Schauer (Verbenaceae), a native species of a dry seasonal tropical forest with pharmacological potential

et al. 2018

View full text Add to dashboard Cite

show abstract

“…High concentrations of PGRs may lead to plant vitrification, which is an unfavorable physiological condition constituting an unquestionable barrier in plant micropropagation [Barbosa et al 2013]. In this research, on 'Burkley' and 'Dover' varieties, BA added in concentration of 3,0 mg·dm -3 to medium caused vitrification on 100% of grown plants [ Barbosa et al 2013].…”

Section: Plant Multiplication and Regenerationmentioning

confidence: 96%

“…In this research, on 'Burkley' and 'Dover' varieties, BA added in concentration of 3,0 mg·dm -3 to medium caused vitrification on 100% of grown plants [ Barbosa et al 2013].…”

Section: Plant Multiplication and Regenerationmentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

View full text Add to dashboard Cite

Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

show abstract

“…According to BARRUETO CID & TEIXEIRA (2010), high cytokinin levels are among the factors that cause vitrification. BARBOSA et al (2013) cultured strawberry in vitro and reported that the increase in the BAP concentration increased the odds of occurrence of hyperhydricity in the plants; concentrations greater than 2.0 mg L -1 of BAP induced greater formation of hyperhydric and oxidized shoots due to increased antioxidant activity and lipid peroxidation, in addition to cellular modifications (deformation of the stomata and epidermal cells).…”

Section: In Vitro Multiplicationmentioning

confidence: 99%

Vernonia condensata Baker: an alternative for large-scale seedling production

Almeida¹,

Oliveira²,

Jacobi³

et al. 2020

Cienc. Rural

View full text Add to dashboard Cite

The increasing use of Vernonia condensata Baker highlights the importance of developing strategies to reduce the impact of exploitation on nature reserves. The aim of this study was to establish a micropropagation protocol to produce homogenous plants with high phytosanitary quality. Apical, nodal, and internodal segments of plants grown in the field were used for in vitro growth. The segments were disinfected in sodium hypochlorite solution (1.0 and 2.0%) for 15 and 30 minutes and then transferred to Petri dishes containing MS culture medium for 30 days. A completely randomized factorial experiment (3 x 2 x 2) with five replicates was designed. After this period, a completely randomized in vitro multiplication experiment was carried out with six treatments (BAP - 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 mg L-1) and six replicates. The shoots obtained in the best treatment were transferred to flasks with rooting medium (MS, MS/2 or MS/4). The experiment was completely randomized with 12 replicates. Microplants were acclimatized in polyethylene terephthalate (PET) bottles filled with autoclaved topsoil. Our results showed that 40.0% of the nodal segments (immersed in 1.0% sodium hypochlorite for 30 minutes) were adequately disinfected and survived. In the in vitro multiplication experiment, the 0.5 mg L-1 concentration of BAP yielded the highest number of shoots and the best vegetative growth. With regard to the assessed characteristics, MS/4 was the best rooting medium, with 100% survival during acclimatization. This study showed that V. condensata in vitro culture might produce 32,000 seedlings in 7 months.

show abstract

Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Cited by 11 publications

References 18 publications

Controlling hyperhydricity in micropropagated plants of Lippia grata Schauer (Verbenaceae), a native species of a dry seasonal tropical forest with pharmacological potential

Controlling hyperhydricity in micropropagated plants of Lippia grata Schauer (Verbenaceae), a native species of a dry seasonal tropical forest with pharmacological potential

In vitro REGENERATION OF Fragraria PLANTS

Vernonia condensata Baker: an alternative for large-scale seedling production

Contact Info

Product

Resources

About