Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

Rodrigues, Donizetti Tomaz; Novais, Roberto Ferreira; Álvarez, V Víctor Hugo; Dias, José Maria Moreira; Otoni, Wagner Campos

doi:10.1590/s0034-737x2012000100002

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Evaluation of in vitro growth of Cattleya trianaei seedlings in MS, MS/2 media and formulated with Peters ® NPK 10-30-20 in different doses recommended the use of the simplified medium with fertilizer Peters ® 3 g L -1 , which presents more balanced amounts of the two sources of nitrogen (NH 4 and NO 3 ) as an alternative and lowcost strategy for in vitro growth of this species (Galdiano Junior;Mantovani;Macedo Lemos, 2012). For seedlings of the hybrid Cattleya walkeriana x C. loddigesii cultivated with different formulations, including Peters ® NPK fertilizer 10-30-20 and NPK 30-10-10 the pieces of evidence shows that the addition of this fertilizer results in a higher yield of a fresh matter of seedlings (Rodrigues et al, 2012a).…”

Section: Resultsmentioning

confidence: 99%

Commercial fertilizers and organic additives in orchid micropropagation

Moraes

Camolesi²,

Palmieri

et al. 2020

PCC&M

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In vitro cultivation strategies promise conservation and micropropagation of vulnerable or economically exciting plants, especially in Orchidaceae. Numerous reports are available for an extensive contingent of orchids of the most varied genera, and the main issue is related to the behavior of species during the process of germination and growth concerning the nutrients used in the culture media. In Orchidaceae, several evidence support that each species responds differently and specifically according to the biotic and abiotic conditions available for in vitro growth. The main concern is to seek simplified culture media that promote germination and the development of healthy plants on a large scale. This study assessed the behavior of Catasetum fimbriatum and Catasetum macrocarpum in culture media containing commercial fertilizers supplemented with organic additives, compared with traditional media, an economically more viable strategy for micropropagation. Seeds were cultured on traditional nutrient media or with commercial fertilizer and organic additives in different combination. The analysis of plant growth after 120 days in vitro showed that the best treatment in both species was the medium containing commercial foliar fertilizer Peters NPK ® 10:30:20 supplemented with potato and banana pulp.

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Section: Resultsmentioning

confidence: 99%

Commercial fertilizers and organic additives in orchid micropropagation

Moraes

Camolesi²,

Palmieri

et al. 2020

PCC&M

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“…Comportamento linear foi observado com a utilização de Peters ® 10-30-20 e MN, com teores máximos de P de 8,2 e 7,0 g kg -1 nas plântulas, valores esses elevados se comparados com os obtidos em Peters ® 30-10-10, em média 3,3 g kg -1 (Tabela 5). Rodrigues et al (2012) observou a mesma tendência para Cattleya walkeriana Gardner. Jones Jr.…”

Section: Resultsunclassified

Concentrações e composições químicas do meio nutritivo para o cultivo in vitro de orquídea

Rodrigues

Novais

et al. 2012

Rev. Ceres

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Concentrações e composições químicas do meio nutritivo para o cultivo in vitro de orquídeaRev. Ceres, Viçosa, v. 59, n.1, p. 1-8, jan/fev, 2012 RESUMO ABSTRACT Chemical composition and component concentrations of culture medium for the in vitro growth of orchidOrchid seedlings grown in vitro respond differently to the different culture media used for this technique. This study compares the traditional Knudson (KC) medium used in orchid in vitro cultivation with a new medium formulation based on the KC medium with modifications, which was named Novais medium (NM), and two other media prepared with NPK fertilizer in the formulations 10-30-20 + micronutrients and 30-10-10 + micronutrients. The media were tested for the in vitro growth of six-month-old orchid seedlings derived from the cross Cattleya walkeriana x Cattleya loddigesii 'Etibaia' and germinated in Knudson C medium. The KC medium was tested in the concentration of 2.0 g L -1 , and the other three media were tested in the concentrations of 0.25; 0.50; 1.00; 1.75; and 2.25 g L -1 . All the media were added with 20 g L -1 of sucrose, solidified with 10 g L -1 agar and pH adjusted to 5.7. NM and Peters ® media were added with 2 g L -1 of activated charcoal and 200 ml L -1 of coconut water. The seedlings were cultured in 320 mL glass jars containing 35 mL of medium. The experiment was maintained in a growth room at 27±2 °C, 16/8 h photoperiod with 48 ì mol m -2 s -1 irradiance. The best responses for production of fresh matter were obtained using NPK fertilizers, in comparison with KC and NM medium. The fresh matter production increased linearly with increasing nutrients doses in both MN and KC media. The orchid seedlings grown in Knudson medium had the lowest means for the variables evaluated. Plântulas de orquídeas cultivadas in vitro respondem de forma distinta aos vários meios de cultura empregados nessa técnica. Este trabalho comparou o meio nutritivo Knudson C, utilizado no cultivo de orquídeas, com o denominado MN e outros dois meios preparados com fertilizantes Peters ® : NPK 10-30-20 + micronutrientes e NPK 30-10-10 + micronutrientes para o cultivo in vitro de plântulas do híbrido Etibaia (Cattleya walkeriana x C. loddigesii), com seis meses de idade, germinadas sobre o meio Knudson C. Os três últimos meios foram testados com as seguintes doses de sais: 0,25; 0,50; 1,00; 1,75; e 2,25 g L -1 , e o meio Knudson C, 2,0 g L -1 . A todos os meios adicionaram-se 20 g L -1 de sacarose, solidificado com 10 g L -1 de ágar, e o pH foi ajustado a 5,7. Aos meios MN e Peters ® foram acrescentados 2 g L -1 de carvão ativado e 200 ml L -1 de água de coco. Foram utilizados frascos de vidro de 320 mL contendo 35 mL de meio nutritivo, e o experimento foi mantido em sala de crescimento a 27 ± 2 ºC, 16/8 h luz/escuro e irrdiância de 48 µmol m -2 s -1 . Melhores respostas para produção de matéria fresca foram obtidas com os fertilizantes NPK, em comparação com os meios Knudson C e MN. A produção de matéria fresca aumentou linearmente com o aumento da dose de nutrientes n...

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Phosphorus-Zinc Interaction and Iron and Manganese Uptake in the Growth and Nutrition of Phalaenopsis (Orchidaceae)

Novais¹,

Novais

et al. 2016

Rev. Bras. Ciênc. Solo

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Visual symptoms of Zn deficiency, induced by excessive P applications, have been observed in commercial orchid nurseries. The supply of other metallic micronutrients, such as Fe and Mn, may also be inadequate in the plant due to high application rates of P. The aim of this study was to investigate this interaction in the nutrition of Phalaenopsis plants. Experimental treatments consisted of three P rates . Dry matter production was evaluated, as well as the levels of P, Zn, Fe, and Mn in both shoots and roots. Higher P rates induced higher shoot dry matter production. However, symptoms of Zn deficiency were observed in plants treated with the highest P rate in the absence of Zn. With increasing P rates, Zn concentrations in the shoots decreased more markedly than in roots, with accumulation of the element in the roots, indicating low Zn translocation to the shoot. A much higher Mn content in shoots (661 mg kg ) suggested that the species is highly tolerant to this micronutrient. The Fe concentrations in the plant were much higher than those indicated in the literature as critical levels for this genus.

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Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

Cited by 4 publications

References 19 publications

Commercial fertilizers and organic additives in orchid micropropagation

Commercial fertilizers and organic additives in orchid micropropagation

Concentrações e composições químicas do meio nutritivo para o cultivo in vitro de orquídea

Phosphorus-Zinc Interaction and Iron and Manganese Uptake in the Growth and Nutrition of Phalaenopsis (Orchidaceae)

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