Indução de embriões somáticos a partir de embriões zigóticos de Heliconia bihai (L.) L. cv. Lobster Claw Two

Abstract

Somatic embryogenesis from zygotic embryos of Heloconia bihai (L.) L. cv. Lobster Claw TwoTropical flowers have contributed to the growth of the cut flower market in Brazil, especially plants of the genus Heliconia. Heliconias are usually propagated vegetatively, although this method can spread diseases and in the near future may severely comprise commercial flower production. In vitro cultivation represents an important potential alternative for guaranteeing the production of high-quality heliconia planting stocks. However, the micropropagation of these plants using stalk apices faces severe problems of endophytic contamination, making it difficult the supply of enough explants. Therefore, alternative techniques for the in vitro production of planting stock were investigated using other explant tissues to induce somatic embryogenesis. Zygotic embryos from immature and mature fruits of H. bihai (L.) L. cv. Lobster Claw Two were cultured in combinations of IAA (0; 5.70, e 11.41 µm L -1 ) and 2,4-D (0; 22.62; 45.24; 67.86 e 90.48 µm L -1 ) to induce somatic embryos during 90 days of culture. Histological analyses showed that somatic embryos were formed only with zygotic embryos derived from mature fruits cultivated in the absence of growth regulators.

Effect of half-strength MS medium supplemented with plant growth regulators on in vitro response in ‘Bird of Paradise’ (Strelitzia reginae L.)

“…Similar results were also observed by Pant & Thapa (2012) [13] in orchids and souza (2010) [28] found that the best shoot formation was observed in a combination of BAP and GA 3 . These same results were in conformity with findings of Ulisses (2010) [32] who observed that ½ MS medium promoted best shoot formation in Heliconia. Development of shoots was observed at 45 days after inoculation in Heliconia (Araujo, 2012) [27] .…”

“…The lowest percentage (45%) of results was recorded in the Control (C), T 2 Gibberellic acid (GA 3 ) @ 3 mg L -1 , T 4 GA 3 @ 1mg L -1 + Benzyladenine (BA) @ 1 mg L -1 treatments. The same result was obtained by Ulisses (2010) [32] in Heliconia bihai L. with use of media containing sucrose. Our results showed that a GA 3 and BA significantly improved in vitro germination of embryos from S. reginae.…”

Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae) cultivados in vitro

“…Lobster Claw Two (Ulisses et al, 2011) e em maracujá -Passiflora edulis Sims (Pinto et al, 2012), estando intimamente ligados aos fatores supracitados, bem como às condições de cultivo e ao tipo de explante utilizado.…”

Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy

“…Somatic embryogenesis is generally induced by strong auxins i.e. more stable to degradation (ULISSES et al, 2011). In anthurium, 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA) are, in that order, the most commonly used auxins.…”

“…De maneira geral, a embriogênese somática é induzida por auxinas fortes, ou seja, mais estáveis à degradação (ULISSES et al, 2011). Em antúrio, o ácido 2,4-diclorofenóxiacético (2,4-D) e o ácido naftalenoacético (ANA) são, nesta ordem, as auxinas mais utilizadas.…”