Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.
A Mielomeningocele (MMC) é uma malformação embrionária do sistema nervoso central, que ocorre nas primeiras quatro semanas de gestação. É decorrente de uma falha no fechamento do tubo neural. A Mielomeningocele é a segunda causa de deficiência do aparelho locomotor em crianças. Estudos recentes revelam a influência de grandes fatores genéticos, ambientais e nutricionais, como a deficiência de ácido fólico no início da gestação. Tem como objetivo observar as grávidas, neonatos e crianças através da literatura com o olhar atencioso com a doença Mielomenigocele. O método é uma revisão integrativa da literatura, as bases de dados utilizadas foram SciELO, LILACS e PUBMED. Após aplicação dos critérios de inclusão e exclusão, a amostra do estudo foi composta por 11 artigos, 77% deles foram publicados no ano de 2020 e 2021, onde estes estudos diferenciaram-se com o objetivo de cada artigo sendo observado com frequência entre alguns artigos a cirurgia fetal aberta para correção de Mielomeningocele (MMC). Através da realização desta revisão foi possível evidenciar na literatura a assistência de enfermagem as grávidas, neonatos e crianças através da literatura com o olhar atencioso com a doença Mielomenigocele.
Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.
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