Conservação De Sementes Do Cafeeiro: Resultados Discordantes Ou Complementares?

Gentil, Daniel Felipe de Oliveira

doi:10.1590/s0006-87052001000300001

Cited by 16 publications

(16 citation statements)

References 13 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The percentage of emergence reduction was observed for the seedlings when the seeds were subjected to fast drying with water content of 15% (Table 6). This behavior was similar to the one found by Gentil (2001), who, by drying coffee beans at a temperature of 30 °C, has observed an inferiority in the performance of the seeds with 16% humidity in relation to the ones that presented 10% and 34%. during germination and also in desiccation tolerance during seed development (Manfre et al, 2009).…”

Section: Resultssupporting

confidence: 89%

Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

et al. 2015

View full text Add to dashboard Cite

Physiological quality and expression of genes in seeds ofHandroanthus serratifolius subjected to drying ABSTRACT -Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality. Index terms: isoenzymes, enzymes, qRT-PCR, Handroanthus serratifolius.Qualidade fisiológica e expressão de genes em sementes de Handroanthus serratifolius submetidas à secagem RESUMO -Sementes de ipê-amarelo são consideradas ortodoxas e dispõem de mecanismos que auxiliam na tolerância à dessecação, como proteínas resistentes ao calor e sistemas de proteção contra radicais livres. Para investigar o efeito da secagem na qualidade das sementes de ipê-amarelo coletadas com 28% de teor de água utilizaram-se dois métodos: secagem lenta (30 ºC) e secagem rápida (45 ºC) até atingirem teores de água na massa de sementes iguais a 20%, 15%, 10% e 5%. A qualidade das sementes foi avaliada por meio de testes de germinação e vigor. Foi avaliada a expressão das proteínas resistentes ao calor e das enzimas catalase, esterase e isocitrato liase. A expressão dos genes sHSP17.5, CAT3 e ICL6 foi quantificada por meio da técnica de qRT-PCR. Maiores valores de germinação e vigor foram obtidos em sementes com teor de água de 20%, submetidas à secagem rápida. Houve a indução de expressão de proteínas resistentes ao calor nos dois métodos de secagem. Ocorreu variação no padrão eletroforético das enzimas catalase, esterase e isocitrato liase entre os diferentes tratamentos. A expressão de genes varia em função dos métodos de secagem e teores de água das sementes. A secagem até os teores de água entre 15 e 10% compromete a qualidade fisiológica das mesmas.Termos para indexação: isoenzimas, enzimas, RTqPCR, ipê-amarelo.

show abstract

Section: Resultssupporting

confidence: 89%

Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Ellis, Roberts (1990, 1991);Gentil (2001), and Hong and Ellis (1992) observed that the critical moisture level, considered lethal for coffee seeds, lies between 0.04 and 0.05 g H 2 O g 1 dw. In the present study, however, the embryos of coffee seeds with 5% moisture exhibited high viability in the tetrazolium test, which was not observed in evaluation of normal seedlings in the germination test (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Tolerance of Coffea arabica L. seeds to sub zero temperatures

et al. 2017

View full text Add to dashboard Cite

Preservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H 2 O g -¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H 2 O g -1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H 2 O g -¹ dw and above 0.43 g H 2 O g -¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H 2 O g -¹ dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds.Index terms: Drying; silica gel; saturated saline solutions; freezing; antioxidant enzymes. RESUMOA preservação da qualidade de sementes de café é dificultada pelo comportamento intermediário no armazenamento. Porém, a conservação a longo prazo em temperaturas subzero pode ser conseguido com o ajuste do teor de água das sementes. Objetivou-se neste trabalho avaliar a tolerância de sementes de café ao congelamento, com relação às modificações fisiológicas e enzimáticas. As sementes foram submetidas a dois tipos de secagem, rápida e lenta, até os teores de água de interesse, de 0,67, 0,43, 0,25, 0,18, 0,11, 0,05 g H 2 O g -¹ dw (base seca). Após secagem, as sementes foram armazenadas em temperatura de -20 e de -86 ºC, por 24 horas e por 12 meses, sendo comparadas às sementes armazenadas em câmara fria a 10 ºC. As sementes foram avaliadas pela porcentagem de plântulas normais, plântulas com folhas cotiledonares expandidas, matéria seca de raízes e de hipocótilos e viabilidade dos embriões no teste de tetrazólio. A expressão das enzimas superóxido dismutase, catalase e peroxidase foi avaliada por meio de análise eletroforética. Apenas as sementes secadas lentamente até 0,18 g H 2 O g -¹ dw apresentam relativa tolerância ao armazenamento a -20 °C por 12 meses. Sementes de café não toleram o armazenamento à temperatura de -86 ºC por 12 meses. Umidades abaixo de 0,11g H 2 O g -¹ dw ...

show abstract

“…Las semillas ortodoxas, son aquellas que toleran la deshidratación, hasta niveles de humedad menores del 5% y las semillas recalcitrantes toleran la deshidratación entre 15 y 50% de humedad (Gentil 2001, Nkang 2002, Sánchez-Arellano et al 2011. Dada la importancia de estos aspectos en el ámbito de la siología y tecnología de semillas, se han desarrollado diferentes protocolos para evaluar la viabilidad y vigor de las semillas, así como para …”

Section: Ríos-garcía Et Al Viabilidad Y Germinación De áRboles Tropiunclassified

Efecto del almacenamiento sobre la viabilidad y germinación de dos especies arbóreas tropicales

Garcia

García

Moreno

et al. 2018

Ecosist. Recur. Agropec.

View full text Add to dashboard Cite

RESUMEN. Cochlospermum vitifolium (Willd.) Spreng. y Quararibea funebris (La Llave) Vischer, son árboles tropicales nativos de México con importancia ecológica, social y económica; de los que no se tiene una práctica sustentable para el manejo. El objetivo fue evaluar la viabilidad y la germinación en semillas almacenadas a 0, 3, 6, 9 y 12 meses. Las semillas a los 0 meses de almacenamiento fueron viables en un 99.66 y 99.33% para C. vitifolium y Q. Funebris, respectivamente, mientras que a los 12 meses se tuvo disminución de la viabilidad, presentando una germinación nal del 71.3% para C. vitifolium y 61.3% para Q. Funebris.Palabras clave: Cochlospermum vitifolium, Quararibea funebris, Reserva de la Biosfera Selva el Ocote, Chiapas, recurso orístico ABSTRACT. Cochlospermum vitifolium (Willd.) Spreng. and Quararibea funebris (La Llave) Vischer are tropical trees native to Mexico with ecological, social and economic importance; however, there is no sustainable management practice for these species. The objective was to evaluate viability and germination in seeds stored at 0, 3, 6, 9 and 12 months. The seeds at 0 months of storage were 99.66 and 99.33% viable for C. vitifolium and Q. Funebris, respectively, while at 12 months there was a decrease in viability, exhibiting a nal germination of 71.3% for C. vitifolium and 61.3% for Q. Funebris.

show abstract

Conservação De Sementes Do Cafeeiro: Resultados Discordantes Ou Complementares?

Cited by 16 publications

References 13 publications

Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying

Tolerance of Coffea arabica L. seeds to sub zero temperatures

Efecto del almacenamiento sobre la viabilidad y germinación de dos especies arbóreas tropicales

Contact Info

Product

Resources

About