Identificação de parentais e híbridos entre Vitis vinifera e Vitis rotundifolia utilizando polimorfismo enzimático e marcador RAPD

Sawazaki, Haiko Enok; Pommer, Celso Valdevino; Passos, Ilene Ribeiro da Silva; Terra, Maurilo Monteiro; Pires, Erasmo José Paioli

doi:10.1590/s0006-87051996000200003

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…4). Similar results were reported by Royo et al (1994) and Sawazaki et al (1996), who observed that only the GOT system of Vitis vinifera demonstrated band migration diversity, with some bands advancing or delaying in relation to the others. These same authors suggested that this diversity might only be associated with migration speed, as genetic crossings to confi rm Mendelian inheritance were not carried out.…”

Section: Resultssupporting

confidence: 90%

In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos

et al. 2010

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The internal morphology of embryos from immature and mature fruits of Heliconia bihai (L.) L. cv. Lobster Claw Two was examined. Embryos were inoculated into MS media (full MS and ½ MS) and GA3 (0, 2.5 and 5 mg L-1) with either sucrose or glucose. These plantlets were then replicated and transferred to MS medium (full MS or ½ MS) with 0 or 2.5 mg L-1 BAP and their multiplication was evaluated 30 and 45 days after inoculation. The genetic variability of the multiplied plants was estimated using isoenzyme analyses. The internal morphology of the mature embryos revealed their tissues to be in more advanced stages of differentiation than immature embryos. In the conversion phase, 85% of the inoculated embryos developed into plants in the ½ MS medium with sucrose, in contrast to only 41% of the embryos that were cultivated with glucose. In the multiplication phase, plants cultivated in ½ MS medium with 2.5 mg L-1 BAP demonstrated more buds. Isoenzyme analyses showed pattern changes in terms of the color intensity and the migration of some of the bands. These results may be associated with differences in the ages of the mother plants and of the plantlets obtained in vitro.

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Section: Resultssupporting

confidence: 90%

In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos

et al. 2010

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Muscadiniana

Vasanthaiah

Thangadurai²,

Basha³

et al. 2010

Wild Crop Relatives: Genomic and Breeding Resources

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Giemsa staining and fluorescent chromosome banding in someVitisL. species

Pinto-Maglio¹,

Pommer

Pierozzi³

2010

Caryologia

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Giemsa staining technique and fl uorescent chromosome banding with CMA 3 and DAPI were applied to the mitotic chromosomes of Vitis champinii, V. cinerea, V. girdiana, V. labrusca, V. rotundifolia, V. rupestris and V. vinifera for the purpose of chromosome measurement and constitutive heterochromatin characterization at the cytochemical level, respectively. Both fl uorescent CMA and conventional Giemsa staining constituted a valuable tool for chromosome characterization. Karyomorphometric data obtained after Giemsa staining allowed for an average ideogram and karyotype formulae based on chromosome length for the species. V. champinii and V. girdiana distinguished from the other fi ve species by means of the total haploid chromosome length, by the longest chromosome length and by the average chromosome length. The seven grape species have moderate chromosome asymmetry values and were classifi ed on 2A Stebbins' category. Positive CMA bands were seen in all species. V. girdiana distinguished from the other species solely by the presence of two CMA + bands, while V. champinii, V. cinerea, V. labrusca, V. rotundifolia and V. rupestris had four bands. V. vinifera showed chromosome heteromorphism for CMA bands. No clearly visible DAPI + band was seen in the species. According to the present observations, it seems that the evolutionary process of speciation involving North American and European Euvitis species studied, resulted in some discrete changes in chromosome measurements and also in heterochromatin base composition of at least one species. These data enlarge the chromosomal information of the genus Vitis and make possible further comparative studies into the Vitaceae family.

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Identificação de parentais e híbridos entre Vitis vinifera e Vitis rotundifolia utilizando polimorfismo enzimático e marcador RAPD

Cited by 3 publications

References 4 publications

In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos

In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos

Muscadiniana

Giemsa staining and fluorescent chromosome banding in someVitisL. species

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