Regional differences in the number and type of myenteric neurons of the ileum of rats: a comparison of techniques of the neuronal evidentiation

Miranda-Neto, Marcílio Hubner de; Molinari, Sônia Lucy; Natali, Maria Raquel Marçal; Sant'Ana, Debora de Melo Gonçalves

doi:10.1590/s0004-282x2001000100012

Cited by 38 publications

(44 citation statements)

References 23 publications

(46 reference statements)

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“…In this study, methylene blue, a pan-neuronal marker (Sant'Ana et al, 1997), was used and results similar to the evaluations of the neuronal subpopulation were observed, that is, higher concentration of neurons in the small gastric curvature. Due to differences in the myenteric neuron population distribution, according to the thickness of the smooth muscle layer, it is necessary that the evaluation of each digestive tract region occurs, even in the organ itself, so that no interpretative error impairs the research result (Miranda-Neto et al, 2001). Sugauara et al (2008Sugauara et al ( , 2009) and Odorizzi suggest that the infection with T. gondii causes changes on the myenteric neurons indirectly, more precisely, by an action of the immune response.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats

Alves¹,

Silva²,

Bianchi³

et al. 2011

Int. J. Morphol.

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SUMMARY:The aim of the study was quantifying and morphologically analyzing the myenteric neurons of the small and large gastric curvatures of the glandular stomach of rats infected the tachyzoites of the Toxoplasma gondii for 30 days. Ten male rats were assigned into two groups: Control Group (CG) and Experimental Group (EG). The animals from the CG received saline solution orally whereas the EG animals received 104 tachyzoites of the T. gondii genotype III strain (BTU II). After 30 days, euthanasia was conducted for the removal of the stomach, which was dissected under the stereomicroscope for removal of the tunica mucosa and the tela submucosa. Whole mounts were stained with Giemsa. Quantification of the myenteric neurons was conducted by using a 40X-objective microscope in 40 microscopic fields for the region of the small gastric curvature and 40 fields for the large gastric curvature of the glandular stomach of the animals from both groups. The cell body of 50 myenteric neurons from each region was measured for each animal. Chronic experimental infection caused by the genotype III strain of Toxoplasma gondii was verified to reduce myenteric neuron density only in the small gastric curvature region of the glandular stomach, not resulting in significant changes in the size of the neurons.

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Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats

Alves¹,

Silva²,

Bianchi³

et al. 2011

Int. J. Morphol.

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“…Ao se realizar estudos quantitativos de neurônios do plexo mioentérico, tem sido recomendada observância das possíveis diferenças existentes entre os diferentes segmentos do sistema digestório [15][16][17] ou, até mesmo, diferenças regionais em um mesmo segmento 13,[15][16][17][18] . Miranda-Neto et al 15 argumentam que, quando utilizados grupos controle e experimental, a inobservância deste aspecto pode comprometer os resultados da pesquisa.…”

Section: Discussionunclassified

Neurônios NADH-diaforase positivos do jejuno de ratos adultos (Rattus norvegicus) desnutridos: aspectos quantitativos

Zanin

Molinari

Sant’Ana

et al. 2003

Arq. Neuro-Psiquiatr.

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RESUMO -Temos por objetivo contribuir com informações sobre os aspectos quantitativos dos neurônios mioentéricos NADH-diaforase positivos do jejuno de ratos submetidos a desnutrição protéica. Foram utilizados 10 ratos (90 dias de idade), divididos em grupos: controle (n=5, ±278g) e desnutrido (n=5, ±280g). Nos 120 dias seguintes, os ratos do grupo controle receberam ração com teor protéico de 22%, os do grupo desnutrido, 8%. Ao final deste período, os ratos do grupo controle pesaram ±394,4g e os desnutrido ±273,5g.O jejuno foi submetido à técnica histoquímica da NADH-diaforase para evidenciação de células nervosas em preparado de membranas. Foram contados os neurônios presentes em 80 campos microscópicos em ambos os grupos. Verificaram-se no controle ±674,6 neurônios e no desnutrido ±1326,8 neurônios; A dieta não alterou a organização dos neurônios entretanto, levou a um menor desenvolvimento corporal nos animais desnutridos, contribuindo para que os neurônios destes sofressem menor dispersão e apresentassem maior densidade por mm 2 . PALAVRAS-CHAVE: desnutrição, jejuno, neurônios mioentéricos, rato. NADH-diaphorase positive neurons of the jejunum of disnurtured adult rats (Rattus norvegicus): quantitative aspectsABSTRACT -We aim at contributing with information on the quantitative aspects of the NADH-diaphorase positive myenteric neurons of the jejunum of adult rats subjected to protein desnutrition. Ten rats aging 90 days were divided into two groups: control (n=5, ±278 g) and disnurtured (n=5, ±280 g). In the following 120 days, the rats from the control group had chow with 22% protein level, and those from the disnurtured group, with 8% protein level. After this period, the control rats weighted ±394.4g and the disnurtured ±273.5g. The jejunum was subjected to the histochemical technique of the NADH-diaphorase to stain nerve cells in whole-mounts. The neurons found in 80 microscopic fields of both groups were counted. In the control ±674.6 neurons were observed, and ±1326.8 neurons were counted in the disnurtured group. The low-protein diet did not alter the organization of the neurons, but led to a smaller body growth in the disnurtured animals, preventing neuronal dispersal and leading to a greater density per mm 2 .

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“…Similarly, the plexus organization remained unchanged among the groups studied (C, CS, DS, and D), not changing either with the diabetes onset and or by the AA-supplementation. The NADPH-dp neurons were distributed in a sparse way inside the ganglion in all groups, occupying the peripheral region of the ganglions as described by Miranda-Neto et al (2001).…”

Section: Discussionmentioning

confidence: 97%

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

et al. 2009

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295Pesq. Vet. Bras. 29(4): 295-302, abril 2009 RESUMO.-[Efeitos da suplementação do ácido ascórbico nos neurônios mientéricos do íleo de ratos diabéticos induzidos por estreptozootocina.] A exacerbação do estresse oxidativo e da via do poliol que comprometem o plexo mioentérico são alterações metabóli-cas características do diabetes. O ácido ascórbico (AA) é antioxidante e inibidor da aldose redutase, podendo atuar como neuroprotetor. Verificaram-se os efeitos da suplementação com AA sobre o número e a área do perfil do corpo celular (PC) de neurônios mioentéricos em ratos diabéticos induzidos por estreptozootocina. Formaram-se quatro grupos com cinco animais cada: normoglicêmico (C); diabético (D); diabético tratado com AA (DS); e normoglicêmico tratado com AA (CS). Três vezes por semana administrou-se 50mg de AA para cada animal (grupos DS e CS). Após 90 dias e eutanásia com tiopental, o íleo foi coletado e processado para a técnica da NADPHdiaforase. Não se observaram diferenças (P>0,05) na densidade neuronal entre os grupos. A área do PC foi menor (P<0,05) para os grupos DS e CS, com incidência maior de neurônios com área do PC superior a 200μm 2 para os grupos C e D. Concluiu-se que o AA não influenciou a densidade neuronal do íleo, mas foi neuroprotetor The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200μm 2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200μm 2 .INDEX TERMS: Vitamin C, diabetes, intestine, myenteric plexus.

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Regional differences in the number and type of myenteric neurons of the ileum of rats: a comparison of techniques of the neuronal evidentiation

Cited by 38 publications

References 23 publications

Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats

Toxoplasma gondii Induces Death of Gastric Myenteric Neurons in Rats

Neurônios NADH-diaforase positivos do jejuno de ratos adultos (Rattus norvegicus) desnutridos: aspectos quantitativos

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

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