Morphological and quantitative analysis of the neurons of the myenteric plexus of the cecum of streptozotocin-induced diabetic rats

Zanoni, Jacqueline Nelisis; Miranda-Neto, Marcílio Hubner de; Bazotte, Roberto Barbosa; Souza, Romeu Rodrigues de

doi:10.1590/s0004-282x1997000500004

Cited by 53 publications

(68 citation statements)

References 15 publications

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“…the absence of a significant difference in mean neuronal number for the large gastric curvature is related to the smaller density of neurons and the larger heterogeneity of their distribution when compared with the small gastric curvature; nevertheless, there was an undeniable neuronal decrease in the corpus of the stomach of diabetic rats. Some authors 11,12 observed a neuronal decrease in the cecum and duodenum, respectively, of rats with streptozotocininduced diabetes. Conversely, other authors 9 did not find a reduction in neuron number in the proximal colon of rats under the same experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

Quantitative study of the myenteric plexus of the stomach of rats with streptozotocin-induced diabetes

Fregonesi

Miranda-Neto

Molinari

et al. 2001

Arq. Neuro-Psiquiatr.

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-The purpose of the present study was to investigate the morphological and quantitative alterations of the myenteric plexus neurons of the stomach of rats with streptozotocin-induced chronic diabetes and compare them to those of non-diabetic animals. Samples from the body of the stomach were used for wholemount preparations stained with NADH-diaphorase and for histological sections stained with hematoxylineosin. It was observed that diabetes cause a significant decrease on the number of neurons.KEY WORDS: myenteric plexus, diabetes, rat.Estudo quantitativo do plexo mientérico do estômago de ratos com diabetes induzido por estreptozootocina RESUMO -A proposta deste trabalho foi estudar as alterações morfológicas e quantitativas dos neurônios do plexo mientérico do estômago de ratos com diabetes induzido por estreptozootocina e estabelecer uma comparação com animais não diabéticos. Amostras do corpo do estômago foram submetidas a preparados de membrana corados pelo método da NADH-diaforase e a cortes histológicos corados por hematoxilinaeosina. Observou-se que o diabetes provoca significante redução no número de neurônios. Diabetes mellitus is a pathological condition in which several physiological abnormalities are observed, such as neuropathies that affect the central nervous system, the peripheral nerves and the autonomic nervous system 1 . Streptozotocin-induced diabetes causes a cycle of events in the nerve cells of the myenteric plexus that begins within one to three days, with the development of chromatolysis followed by regenerative changes over the next six weeks, with some continuous, albeit occasional, ganglionic degeneration 2 ; changes in the innervation of the ileum and colon 3 are reported, as well as a decrease on the neuronal activity 4 . Studies on streptozotocininduced diabetic rats have emphasized the alterations that occur in the small and large intestines. However, it should be remembered that alterations in the neurons of the myenteric plexus, in addition to changing intestinal peristalsis, can affect stomach motility, leading to dilation and retarded gastric emptying, interfering with the coordination between stomach and duodenum and impairing the digestive process 5 . PALAVRAS-CHAVEIn a diabetic ketonuric Chinese hamsters examination of the gastrointestinal tract was observed that the animals had distention and atony of the stomach and intestines when compared with control animals 6 . In humans, among the clinical manifestations of autonomic neuropathy, there is motor impairment of the stomach, which can lead to anorexia, nausea, vomiting and persisting fullness, characterizing gastric debilitation 1,5 . It is also noteworthy that diabetic gastric atony is similar to that observed after vagotomy 5 .Considering the extensive development of the myenteric plexus and its importance for the control of gastric and intestinal functions, and suspecting that diabetes may affect these neurons, in this way contributing to the gastric disturbances, we undertook the present study to determine ...

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Section: Resultsmentioning

confidence: 99%

Quantitative study of the myenteric plexus of the stomach of rats with streptozotocin-induced diabetes

Fregonesi

Miranda-Neto

Molinari

et al. 2001

Arq. Neuro-Psiquiatr.

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“…Insulin deficiency is also an explanation for the reduced dimensions observed here, once insulin is an anabolic hormone and, as such, a promoter of cellular development. On the other hand, there are reports that rats subjected to experimental diabetes have dimensional and weight increases of the small and large intestines 2,4,6,7,12 . The absence of hypertrophy of the proximal colon in this study could be related to factors such as the duration of the experimental period, the age of the animals and the moderate diabetic state.…”

Section: Discussionmentioning

confidence: 99%

“…Among those systems under investigation are the gastrointestinal tract and its intrinsic enteric nervous system, the neuronal network responsible for the control of the activities of the bowel. It is reported, for instance, that the myenteric neurons of the stomach, duodenum and cecum are numerically reduced in diabetes [1][2][3] , and that specific neurochemical groups show response patterns to diabetes which depend on the intestinal segment and the duration of the diabetic state [4][5][6][7] . These neuronal changes, as well as those associated to the autonomic innervation of the gut [8][9][10] , stand among the responsible by the clinical gastrointestinal symptoms of diabetes [11][12][13] .…”

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confidence: 99%

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan

Molinari

Neto

2002

Arq. Neuro-Psiquiatr.

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-The effects of acute diabetes on the density and size of the myenteric neurons of the proximal colon of adult rats were investigated. The injection of streptozotocin was followed by a period of observation of seven days, during which the diabetic animals showed weight loss, excessive food and water intake, large urinary debt and hyperglicemia. The whole-mounts from the proximal colon were stained with the techniques of Giemsa and of the NADH-diaphorase, and the employment of these techniques made it possible to verify a decrease on the neuronal density and on the cell body size of the myenteric neurons in the colon of the diabetic rats. These observations were discussed in terms of the pathophysiology of the diabetes and the experimental protocol.KEY WORDS: acute diabetes, myenteric neurons, proximal colon.Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos RESUMO -Foram investigados os efeitos do diabetes agudo sobre a densidade e o tamanho dos neurônios mioentéricos do colo proximal de ratos adultos. À injeção de estreptozootocina seguiu-se um período de observação de sete dias, durante os quais os animais diabéticos apresentaram perda de peso, ingestão excessiva de alimento e água, grande débito urinário e hiperglicemia. Os preparados de membrana do colo proximal foram corados pelas técnicas de Giemsa e da NADH-diaforase. A aplicação dessas técnicas permitiu constatar uma redução da densidade neuronal e do tamanho do corpo celular dos neurônios mioentéricos no colo dos ratos diabéticos. Essas observações foram discutidas em termos da patofisiologia do diabetes e do protocolo experimental. The research on the changes induced by experimental diabetes on the several tissues and organs of laboratory animals is quite large. Among those systems under investigation are the gastrointestinal tract and its intrinsic enteric nervous system, the neuronal network responsible for the control of the activities of the bowel. It is reported, for instance, that the myenteric neurons of the stomach, duodenum and cecum are numerically reduced in diabetes 1-3 , and that specific neurochemical groups show response patterns to diabetes which depend on the intestinal segment and the duration of the diabetic state 4-7 . These neuronal changes, as well as those associated to the autonomic innervation of the gut 8-10 , stand among the responsible by the clinical gastrointestinal symptoms of diabetes [11][12][13] . PALAVRAS-CHAVERecently, we described an increase in the NADHdiaphorase positive myenteric neuronal population in the duo...

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“…E-mail:jnzanoni@uem.br with induced colitis. Buttow et al (8) and Zanoni et al (38) found, respectively, a reduction in density of myenteric neurons in the duodenum and in the cecum of diabetic rats.…”

Section: Introductionmentioning

confidence: 97%

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

Zanoni

Tronchini

Moure

et al. 2011

Arq. Gastroenterol.

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-Context -Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy.The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. Objective -To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. Methods -The animals were divided in four groups (n = 5): non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm 2 in the cecum and 3.6 mm 2 in the duodenum of each animal. Results -After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05). We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine) (P<0.05). In the cecum, that preservation was not evident. Conclusion -Supplementation with L-glutamine (1%) promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus.

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Morphological and quantitative analysis of the neurons of the myenteric plexus of the cecum of streptozotocin-induced diabetic rats

Cited by 53 publications

References 15 publications

Quantitative study of the myenteric plexus of the stomach of rats with streptozotocin-induced diabetes

Quantitative study of the myenteric plexus of the stomach of rats with streptozotocin-induced diabetes

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats

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