Neuron number in the myenteric plexus of the ascending colon of rats: a comparative study using two staining techniques

Sant’Ana, Débora de Mello Gonçales; Miranda-Neto, Marcílio Hubner de; Molinari, Sônia Lucy; Sant'anna, Marco Antônio

doi:10.1590/s0004-282x1997000300018

Cited by 10 publications

(17 citation statements)

References 17 publications

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“…On the other hand, the technique of the NADH-d stains only a fraction of the myenteric neuronal population. Although there are reports that it stains almost all the myenteric neurons 27,31 , this has not been the case in our research group 14,28 . In this study, the number of NADH-d positive neurons in the proximal colon was of 57-66% that found with Giemsa.…”

Section: Discussioncontrasting

confidence: 92%

“…The neuronal counts made with Giemsa yielded a neuronal density corresponding to 29,000 neurons/cm 2 of proximal colon, a value much similar to that found in other age-and weight-matched rats 28 . In younger rats, is was found a number of neurons/cm 2 larger than this 30 , which can be related to the smaller intestinal area and correspondingly lesser neuronal dispersal.…”

Section: Discussionsupporting

confidence: 80%

“…Although not attaining significance, these circumferential differences were observed in other instances 24 , correlated to the fact that the intermediate region of the colon has a thicker layer of longitudinal smooth muscle, analogous to the human, which must require a larger neuronal population for its innervation and control 24 . This kind of regional variation was also observed in other segments of the bowel [25][26][27][28][29] .…”

Section: Discussionsupporting

confidence: 61%

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Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan

Molinari

Neto

2002

Arq. Neuro-Psiquiatr.

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-The effects of acute diabetes on the density and size of the myenteric neurons of the proximal colon of adult rats were investigated. The injection of streptozotocin was followed by a period of observation of seven days, during which the diabetic animals showed weight loss, excessive food and water intake, large urinary debt and hyperglicemia. The whole-mounts from the proximal colon were stained with the techniques of Giemsa and of the NADH-diaphorase, and the employment of these techniques made it possible to verify a decrease on the neuronal density and on the cell body size of the myenteric neurons in the colon of the diabetic rats. These observations were discussed in terms of the pathophysiology of the diabetes and the experimental protocol.KEY WORDS: acute diabetes, myenteric neurons, proximal colon.Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos RESUMO -Foram investigados os efeitos do diabetes agudo sobre a densidade e o tamanho dos neurônios mioentéricos do colo proximal de ratos adultos. À injeção de estreptozootocina seguiu-se um período de observação de sete dias, durante os quais os animais diabéticos apresentaram perda de peso, ingestão excessiva de alimento e água, grande débito urinário e hiperglicemia. Os preparados de membrana do colo proximal foram corados pelas técnicas de Giemsa e da NADH-diaforase. A aplicação dessas técnicas permitiu constatar uma redução da densidade neuronal e do tamanho do corpo celular dos neurônios mioentéricos no colo dos ratos diabéticos. Essas observações foram discutidas em termos da patofisiologia do diabetes e do protocolo experimental. The research on the changes induced by experimental diabetes on the several tissues and organs of laboratory animals is quite large. Among those systems under investigation are the gastrointestinal tract and its intrinsic enteric nervous system, the neuronal network responsible for the control of the activities of the bowel. It is reported, for instance, that the myenteric neurons of the stomach, duodenum and cecum are numerically reduced in diabetes 1-3 , and that specific neurochemical groups show response patterns to diabetes which depend on the intestinal segment and the duration of the diabetic state 4-7 . These neuronal changes, as well as those associated to the autonomic innervation of the gut 8-10 , stand among the responsible by the clinical gastrointestinal symptoms of diabetes [11][12][13] . PALAVRAS-CHAVERecently, we described an increase in the NADHdiaphorase positive myenteric neuronal population in the duo...

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Section: Discussioncontrasting

confidence: 92%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 61%

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Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan

Molinari

Neto

2002

Arq. Neuro-Psiquiatr.

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“…Experimental desnutrition is a fruitful area for the investigation of neuronal plasticity and the interdependence of neuronal and non-neuronal mechanisms 7 . Quantitative and morphological evaluations of the neurons of the myenteric plexus in malnourished rats have been demonstrating a larger neuronal density in the small intestine [8][9][10][11] and in the colon 12 , both due to the smaller growth of the animals and their bowels, which lead to lesser neuronal spreading. Most of the authors reviewed offered to the animals diets with protein deficiency but included minerals and vitamins of the complex B [8][9][10][11][12] .…”

mentioning

confidence: 99%

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana

Molinari

Miranda-Neto

2001

Arq. Neuro-Psiquiatr.

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-The aims of this work were to evaluate the effects of the deficient ingestion of protein and vitamin B on the biochemical and hematologic parameters and on the NADH-and NADPH-diaphorase positive myenteric neurons. The control animals (n=10) received commercial chow and the experimental rats (n=10) received chow with protein level reduced to 8% during 120 days. At the time of killing blood was collected for assessment of the blood and hematologic parameters and the ascending colon for quantitative analysis of the neurons of the myenteric plexus. It was observed that the reduction of the protein level to 8% coupled to the reduction of the levels of vitamin B in adult rats neither led to qualitative or quantitative changes on red or white blood cells, nor decreased globulin levels, induced the formation of edema or gave rise to clinical signs typical of protein or vitamin B deficiency. On the other hand, the experimental protocol led to less weight gain, change on the body composition with fat deposition; decrease of the values of serum total protein and albumin; reduction of the area of colon and density of nitrergic and NADH-diaphorase myenteric neurons inferior to the expected.KEY WORDS: enteric neurons, proteic desnutrition, ascending colon, hematology, vitamin B.Efeitos da deficiência de proteínas e vitamina B sobre parâmetros sanguíneos e neurônios mioentéricos do colo de ratos adultos RESUMO -Os objetivos deste trabalho foram avaliar as repercussões da ingestão deficiente de proteínas e vitaminas do complexo B sobre parâmetros bioquímicos e hematológicos e neurônios mientéricos NADH e NADPH-diaforase positivos. Os animais controle (n=10) receberam ração comercial e os animais experimentais (n=10) ração com teor protéico reduzido para 8% durante 120 dias. Durante o sacrifício, coletou-se o sangue dos animais para avaliação de parâmetros sanguíneos e hematológicos e o colo ascendente para a análise quantitativa dos neurônios do plexo mioentérico. Observou-se que a redução do teor de proteínas na dieta para 8% associado a redução no teor de vitaminas do complexo B em ratos adultos, não causa alterações qualitativas e quantitativas das células sanguíneas das séries vermelha e branca, não leva a redução do valor da globulina nem a formação de edema ou ao surgimento de sinais clínicos característicos das carências de proteínas e vitaminas do complexo B. Por outro lado causa: menor ganho de peso corporal; alteração da composição corporal com acúmulo de gordura; redução nos valores de proteína total e albumina; redução na área do colo e evidenciação de densidade de neurônios nitrérgicos e NADH-diaforase positivos inferior ao esperado.PALAVRAS CHAVE: neurônios entéricos, desnutrição protéica, colo ascendente, hematologia, vitamina B. The utilization of animal models in studies of food restriction with known and controlled diets has as an advantage the improved knowledge about the many aspects of the human desnutrition 1 . Research on desnutrition aimsat simulating natural conditions, which involve deficienc...

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“…In higher vertebrates, the myenteric plexus is located between the longitudinal and circular strata of the muscle layer of the gastrointestinal segments forming a neuron network gathered predominantly in ganglia interconnected by bundles of nerve fibers (Gabella 1979, 1990, Molinari et al 1994, Sant'Ana et al 1997, Furness 2000, Mari et al 2007, Silva et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

et al. 2009

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295Pesq. Vet. Bras. 29(4): 295-302, abril 2009 RESUMO.-[Efeitos da suplementação do ácido ascórbico nos neurônios mientéricos do íleo de ratos diabéticos induzidos por estreptozootocina.] A exacerbação do estresse oxidativo e da via do poliol que comprometem o plexo mioentérico são alterações metabóli-cas características do diabetes. O ácido ascórbico (AA) é antioxidante e inibidor da aldose redutase, podendo atuar como neuroprotetor. Verificaram-se os efeitos da suplementação com AA sobre o número e a área do perfil do corpo celular (PC) de neurônios mioentéricos em ratos diabéticos induzidos por estreptozootocina. Formaram-se quatro grupos com cinco animais cada: normoglicêmico (C); diabético (D); diabético tratado com AA (DS); e normoglicêmico tratado com AA (CS). Três vezes por semana administrou-se 50mg de AA para cada animal (grupos DS e CS). Após 90 dias e eutanásia com tiopental, o íleo foi coletado e processado para a técnica da NADPHdiaforase. Não se observaram diferenças (P>0,05) na densidade neuronal entre os grupos. A área do PC foi menor (P<0,05) para os grupos DS e CS, com incidência maior de neurônios com área do PC superior a 200μm 2 para os grupos C e D. Concluiu-se que o AA não influenciou a densidade neuronal do íleo, mas foi neuroprotetor The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200μm 2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200μm 2 .INDEX TERMS: Vitamin C, diabetes, intestine, myenteric plexus.

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Neuron number in the myenteric plexus of the ascending colon of rats: a comparative study using two staining techniques

Cited by 10 publications

References 17 publications

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

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