Saturated salt accelerated aging and computerized analysis of seedling images to evaluate soybean seed performance

Yagushi, Juliana Tieme; Costa, Denis Santiago da; Neto, J. de B. França

doi:10.1590/2317-1545v32n2935

Cited by 15 publications

(16 citation statements)

References 17 publications

(16 reference statements)

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“…This occurs because the relative humidity within the plastic boxes with saturated NaCl solution (76%) is lower than that maintained in the traditional test (near 100%), allowing uniform reduction in the speed of water absorption by the seeds, the intensity of deterioration, and the degree of stress placed on the seeds. The effectiveness of the procedure with use of a saturated sodium chloride solution for evaluation of soybean seed vigor was investigated by Marcos-Filho et al 2000, Marcos-Filho et al (2009), and Yagushi et al (2014).…”

Section: Evaluation Of Seed Physiological Potentialmentioning

confidence: 99%

Vigor-S: System for Automated Analysis of Soybean Seed Vigor

Rodrigues¹,

Gomes-Júnior²,

Marcos-Filho³

2020

J. Seed Sci.

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Computerized systems for image analysis are alternatives to decrease the subjectivity and speed of assessment of seed physiological potential. The aim of this study was to determine the efficiency of the Seed Vigor Automated Analysis System (Vigor-S) to identify differences in vigor among soybean seed lots compared to results of the Seed Vigor Imaging System - SVIS® and tests recommended for evaluation of soybean seed vigor. Two cultivars were used, BMX Potência RR and 7166 RSF IPRO, each one represented by ten seed lots with similar germination and vigor differences. The seeds were evaluated regarding germination, vigor (tetrazolium, accelerated aging), seedling emergence in the field and SVIS® and Vigor-S analysis, in three experimental periods. Analysis of variance was used on the data in a completely randomized experimental design (laboratory tests) and in randomized blocks (field tests), and the mean values were compared by the Tukey test (p ≤ 0.05). Separation of the seed lots by Vigor-S was consistent with the results obtained in tests recognized as effective in evaluating the physiological potential of soybean seeds, and there was no interference of the different cultivars on the response pattern for vigor. Analysis through Vigor-S proved to be effective in determination of physiological potential and for composition of quality control programs established by soybean seeds production companies.

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Section: Evaluation Of Seed Physiological Potentialmentioning

confidence: 99%

Vigor-S: System for Automated Analysis of Soybean Seed Vigor

Rodrigues¹,

Gomes-Júnior²,

Marcos-Filho³

2020

J. Seed Sci.

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“…In this context, the observation of color differences, together with the knowledge of several seed characteristics, allow the determination of the presence, location and nature of the damage (Costa et al, 2003) and can be adapted to cryopreservation research. Therefore, this equipment shows techniques that allow the evaluation of storage deterioration by color analysis (Yagushi et al, 2014), which may or may not be associated with the cryopreservation procedure. In addition to color changes, texture attributes have been one of the main characteristics used in the classification of digital images (Aksoy and Haralick, 1998) and, in this study, they could be verified by the GroundEye ® L800 through Haralick descriptors (Felipe and Traina, 2003).…”

Section: Discussionmentioning

confidence: 99%

Conservation of Hibiscus acetosella germplasm by seed cryopreservation

et al. 2019

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Hibiscus acetosella (Malvaceae) is a shrub of great importance for landscaping, food and medicinal purposes. The objective of this study was to preserve H. acetosella germplasm by seed cryopreservation. Half of the seed batch was scarified and the other half was kept intact. Cryopreservation occurred by immersion in liquid nitrogen for 1 hour. Moisture content (MC%), germination percentage (G%), germination speed index (GSI), normal seedling formation (NS%), shoot length (SL), dry matter (DM), biometry and plant survival were evaluated after treatment. MC% ranged between 7.7% and 6.65% in intact and scarified seeds, respectively. Scarification raised G% and GSI compared to intact seeds. Intact and scarified seeds had 100% and 70% NS%, respectively, when not cryopreserved. Cryopreservation reduced NS% to 62% and 12.75%, respectively. The highest SL was observed in intact and non-cryopreserved seeds, with an average of 10.21 cm in height. However, the cryopreservation of intact seeds reduced SL by about 50%, and scarification led to a further reduction, either with (3.32 cm) or without (2.47 cm) cryopreservation. Seedlings from intact and non-cryopreserved seeds showed higher DM in relation to seedlings from cryopreserved seeds. The association of cryopreservation and scarification further reduced DM. The cryopreservation of intact seeds yielded 100% survival at the end of the acclimatization process. However, cryopreservation of scarified seeds reduced the survival percentage to 15%. Changes in color were observed for seeds scarified and subjected to cryopreservation. Thus, cryopreservation is considered an efficient technique for the conservation of intact H. acetosella seeds in the long term.

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“…been successfully reported for several species, such as pumpkin (Silva et al 2017), peanut (Barbosa et al 2016), corn (Castan et al 2018), wheat (Brunes et al 2016), sunflower (Rocha et al 2015), bean (Gomes Junior et al 2014) and soybean (Marcos Filho et al 2009, Wendt et al 2014, Yagushi et al 2014, among others. The commercial systems developed for this purpose are powerful tools for collecting image data and making inferences about seed vigor.…”

Section: Abstract Resumomentioning

confidence: 99%

SAPL®: a free software for determining the physiological potential in soybean seeds1

Medeiros

Pereira

2018

Pesqui. Agropecu. Trop.

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Obtaining image-based information is a powerful approach to capture and quantify seed vigor data. However, commercial systems that facilitate the processing and acquisition of images are often cost prohibitive. This study aimed to evaluate the efficiency of the Seedling Analysis System (Sistema de Análise de Plântulas - SAPL®), in order to analyze the physiological potential of soybean seeds, in comparison with the information provided by vigor tests which are traditionally recommended for this species. Nine lots of soybean seeds were submitted to germination, germination speed, seedling emergence, cold test and electrical conductivity tests. In the SAPL® analyzes, seedlings of four and six days, counted from the beginning of the germination test, were evaluated, resulting in values for seedling length, growth, development uniformity, vigor index and corrected vigor index. The evaluated lots with emergence greater than 90 % showed a vigor index higher than 600 and 800, respectively in the fourth and sixth days. The indexes generated by SAPL®, except for the uniformity index, presented positive and high correlations with the traditional tests (> 0.80). SAPL® is efficient in identifying differences in the vigor of soybean seed lots.

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Saturated salt accelerated aging and computerized analysis of seedling images to evaluate soybean seed performance

Cited by 15 publications

References 17 publications

Vigor-S: System for Automated Analysis of Soybean Seed Vigor

Vigor-S: System for Automated Analysis of Soybean Seed Vigor

Conservation of Hibiscus acetosella germplasm by seed cryopreservation

SAPL®: a free software for determining the physiological potential in soybean seeds1

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