2017
DOI: 10.1590/2236-8906-75/2016
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Abstract: -(Callus induction and betacyanin quantification by HPLC/MS-MS in Alternanthera brasiliana (L.) Kuntze).The objective of this study was to establish a protocol for callus induction and betacyanin production in plants of Alternanthera brasiliana. Explants of A. brasiliana and five combinations of cytokinin and auxin were used for callus induction. Calli were transferred to a Betacyanin Induction Medium (MIB), composed of MS, with 0.5 mg L -1 of thidiazuron (TDZ) and 1 mg L -1 of naphthaleneacetic acid (NAA), an… Show more

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Cited by 4 publications
(3 citation statements)
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“…Plants were collected in the municipalities of Rio Grande, state of Rio Grande dos Sul (2006) (Murashige & Skoog, 1962) ) were tested, totaling four types of medium. Petri dishes were randomly distributed with occasional rotation and kept in the dark at 25 ± 2° C for 20 days and then transferred to light for 10 days where they were kept a 16 hours photoperiod at 25° C ± 2 (Reis et al, 2017).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Plants were collected in the municipalities of Rio Grande, state of Rio Grande dos Sul (2006) (Murashige & Skoog, 1962) ) were tested, totaling four types of medium. Petri dishes were randomly distributed with occasional rotation and kept in the dark at 25 ± 2° C for 20 days and then transferred to light for 10 days where they were kept a 16 hours photoperiod at 25° C ± 2 (Reis et al, 2017).…”
Section: Methodsmentioning
confidence: 99%
“…in gauze and centrifuged at 10.000 g for 20 minutes, at 4° C (Reis et al, 2017). The flavonoid concentration was expressed in μmol of quercetin per gram of fresh mass and the readings were carried out in a spectrophotometer at 330 nm.…”
Section: Methodsmentioning
confidence: 99%
“…Os resultados alcançados no presente estudo são superiores aos relatados por Vasconcelos et al (2012) em trabalhos de calogênese com a mesma espécie, no qual os autores obtiveram as maiores médias (87,50 e 82,50%) com a utilização de 4,52 e 6,78 µM de 2,4-D, respectivamente. Outros autores já descreveram o efeito positivo da combinação de auxinas e citocininas na indução de calos, a exemplo de Reis et al (2017) que ao combinar o uso 2,4-D com CIN sob distintos explantes (segmento foliar e internodal) de Alternanthera brasiliana (L.) Kuntze, relataram taxa máxima (100%) de formação de calos em explantes foliares no período de 30 dias com a utilização de 4,53 µM de 2,4-D combinado com 4,65 µM de CIN. Já Santos et al (2016) verificaram a maior porcentagem (100%) de indução de calos em folhas Piper permucronatum Yunck.…”
Section: Indução De Calosunclassified