In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest

Juras, Monique Cristine R.; Jorge, Jackeline; Pescador, Rosete; Ferreira, Wagner de Melo; Tamaki, Vívian; Suzuki, Rogério Mamoru

doi:10.1590/2175-7860201970014

Cited by 11 publications

(11 citation statements)

References 39 publications

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“…The widest leaf blades were noted when plantlets were cultivated in vitro in the presence of IBA and acclimatized in a mixture of sphagnum moss and substrate for orchids (8.9 mm), in comparison to those cultivated in the presence of IAA and planted into the same type of substrate (7.4 mm). The positive effect of sphagnum moss was observed by Suradinata et al [ 38 ] in Dendrobium Indii × Dendrobium stratiotes and Dendrobium sylvanum , as well as by Juras et al [ 39 ] in Cattleya xanthina . The negative influence of sphagnum moss on the studied feature was noted by Trelka et al [ 23 ] in Phalaenopsis ‘Springield’ and ‘Zagreb’.…”

Section: Resultsmentioning

confidence: 86%

“…A negative influence of sphagnum moss used alone was noted in the presented experiment, which confirms the data obtained by Alves and Smozinski [ 37 ] in Epidendrum ibaguense , or Trelka et al [ 23 ] in Phalaenopsis ‘Springfield’ and ‘Zagreb’. On the other hand, the positive effect of sphagnum moss was noted in the studies of Suradinata et al [ 38 ] in Dendrobium Iindii × Dendrobiium stratiotes and Dendrobium sylvanum , as well as Juras et al [ 39 ] in Cattleya xanthina . The lowest number of leaves was observed in plants that had been cultivated in vitro in the presence of IAA and then grown in the substrate for orchids (3.0).…”

Section: Resultsmentioning

confidence: 99%

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Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Poniewozik

Parzymies

Szot

et al. 2021

Plants

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To obtain healthy and good quality plants from in vitro cultivation, it is necessary to produce plantlets with well-developed rooting systems because they must undergo acclimatization, a final and a very difficult stage of micropropagation. In the present research, the effect of auxins NAA, IAA and IBA in concentrations of 0.5; 1; 2.5 and 5 mg·dm−3 on the Paphiopediluminsigne in vitro rooting was studied, and it was noted that 1 mg·dm−3 of IAA or IBA enabled the obtaining of a lot of rooted and good quality plantlets. The subsequent influence of the two most advantageous auxins on the acclimatization of plantlets in different substrates (sphagnum moss, sphagnum moss + substrate for orchids, substrate for orchids, substrate for orchids + acid peat) was tested, in the means of morphological features of plants and their physiological parameters, i.e., chlorophyll fluorescence (FV, Fm, Fv/Fm), stress enzyme activity (catalase, ascorbate peroxidase), and water balance. Considering all the tested features, it might be stated that the best results were obtained when explants were rooted in vitro in the presence of 1 mg·dm−3 of IAA and then planted ex vitro in substrate for orchids.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Poniewozik

Parzymies

Szot

et al. 2021

Plants

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“…Dewir et al (2019) on Dracaena ombet found that 100% rooting was obtained using half strength MS medium supplemented with 0.5 or 1 mg/l IBA. Juras et al (2019) on Cattleya xanthina showed that the highest concentration of auxin (0.11 ppm NAA) stimulated root development and the production of photosynthetic pigments.…”

Section: Al (2018) On Codiaeum Variegatum In Vitromentioning

confidence: 99%

“…Dewir et al (2018) on Conocarpus erectus found that the type and concentration of applied cytokinins had a significant effect on shoot proliferation responses, and supplementation with BAP increased the rate of shoot proliferation compared with other cytokinins. Juras et al (2019) on Cattleya xanthina showed that a higher concentration of cytokinin (0.50 ppm BA) promoted protein synthesis and the development of shoots. Ghareeb and Taha (2018) found that during the multiplication stage of Antigonon leptopus explants, the highest number of shootlets/explant were obtained when 1.0 mg/l of BAP was added to MS medium.…”

Section: Multiplication Stagementioning

confidence: 99%

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

Sayed¹

2021

Scient. J. of Flowers and Ornament. Plants

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during 2018 and 2019 seasons, with the aim to determine the best protocol of tissue culture technology to propagate Dracaena umbraculifera plant. For the possibility of spreading its cultivation to preserve it from extinction. As this plant is found in very few numbers, and it is difficult to form seeds under the climatic conditions in Egypt. Results obtained stated that using Clorox at 40% for 20 min gave the highest significant survival percentage (86.13%). For multiplication stage, benzyl adenine (BA) proved its superiority over kinetin in the production of a greater number and length of shoots. The highest number of shoots (3.20) was obtained with BA at 1.5 ppm and the highest shoots length (2.23 cm) was recorded with BA at 1.3 ppm. For rooting stage, the use of 1-naphthaleneacetic acid (NAA) at 0.3 ppm produced the highest values of roots number/plantlet (3.13) and root length (5.93 cm), while the lowest values were recorded when using indole-3-butyric acid (IBA) at 0.1 ppm. For acclimatization stage, the type of pot media had a significant effect. Using peat moss only under greenhouse condition gave the highest survival percentage (100%) and the highest values for all the measurements such as plant height (cm), number of leaves/plant, root length (cm), number of roots/plant, fresh and dry weights of leaves and roots (g/plant). Likewise, for the content of the leaves of total chlorophyll, carotenoids and the percentage of total soluble sugars. So, it can be recommended to make a protocol by using the tissue culture technique for propagating of Dracaena umbraculifera plant as follows: use Clorox at 40% for explant sterilization, using 1.5 ppm of BA in the multiplication stage, using 0.3 ppm of NAA in the rooting stage and using peat moss only to grow dracaena plant in the acclimatization stage to get plants with good growth characteristics.

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“…However, many orchid species are threatened due to deforestation and predatory collection in their natural habitats, making it essential to utilize techniques for mitigating this problem. One of these is in vitro cultivation that allows a high germination percentage in a short period, thus contributing to the conservation of native or threatened species (Juras et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

In vitro cultivation and acclimatization of Miltonia flavescens (Lindl.) Lindl. with ora-pro-nobis extract (Pereskia aculeata Mill.)

Zanella

Stefanello

Sanches

2021

RSD

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The aim of this study was to evaluate the effect of aqueous ora-pro-nobis (Pereskia aculeata Mill.) extract on in vitro growth, survival rate, and acclimatization of Miltonia flavescens. Crude ora-pro-nobis extract was produced by infusing 10 g of dry leaves fractionated in 100 mL of distilled water. The treatments consisted of the crude extract (100%) and dilutions of 75%, 50%, and 25% added to Murashige and Skoog ½ culture medium (control treatment: absence of extract) where seedlings germinated in vitro were cultivated. After 270 days of cultivation, growth data were collected, and the plants acclimated. The survival rate was evaluated every 30 days, and the growth of the aerial part and root system were measured after 120 days of acclimatization. The data were submitted to analysis of variance and a test of the separation of means (Skott-Knott). For the number of leaves, the 100% treatment had the highest average value, differing statistically from the other treatments. For the length of the root system and fresh mass, the highest averages occurred in the control and 100% treatments. For the number of roots and shoots, there were no differences between treatments. After 120 days of acclimatization, plants from the 100% treatment had the lowest survival percentage, and plants from the 25% and 50% treatments had the greatest increase in the growth of the aerial part and root systems, demonstrating these treatments would be beneficial for the cultivation of this species.

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In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest

Cited by 11 publications

References 39 publications

Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

In vitro cultivation and acclimatization of Miltonia flavescens (Lindl.) Lindl. with ora-pro-nobis extract (Pereskia aculeata Mill.)

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