Oleic acid variation and marker-assisted detection of Pervenets mutation in high- and low-oleic sunflower cross

Dimitrijević, Aleksandra; Imerovski, Ivana; Miladinović, Dragana; Cvejić, Sandra; Jocić, Siniša; Zeremski, Tijana; Sakač, Zvonimir

doi:10.1590/1984-70332017v17n3a36

Cited by 32 publications

(27 citation statements)

References 33 publications

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“…Although constraints have limited the use of MAS in plant breeding programs in Brazil (Sakiyama et al 2014), use of molecular markers is effective for some traits, mainly in transfer of major genes (Santana et al 2014, Yamanaka et al 2013, Dimitrijević et al 2017. The use of backcrossing may be the best approach to transfer low linolenic acid and high oleic acid contents to elite cultivars.…”

Section: Resultsmentioning

confidence: 99%

Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Bueno

God

Prata

et al. 2018

Crop Breed. Appl. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Bueno

God

Prata

et al. 2018

Crop Breed. Appl. Biotechnol.

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“…Manuscript to be reviewed Apart from their essential role in nutrition, sunflower and rapeseed oils have a number of industrial applications: they can be used as basic components in polymer synthesis, serve as the source of biofuel, or be used as emulsifiers or lubricants (Dimitrijević et al, 2017). Production of selection hybrids with changed oil properties is one of the key objectives of the oilseed crop breeding (Jocic´, Miladinovic´ & Kaya, 2015).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops (v0.2)"

2019

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Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher number of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain Fas. The longest FA detected using GC-FID was a FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has a great potential to be used for the assessment of the FA profile of oil crops.

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“…High (>80%) or mid oleic type (60-70%) sunflower oil is significant in the world due to high oleic acid sunflower oil is more appropriate for frying and beneficial for health (Evci et al 2016). High oleic oils are also significant in food processing due to being more stable in exposure to high temperatures (Dimitrijevic et al 2017). Non-food applications in particular require oleic acid content that is stable and higher than 90% (Vannozzi 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, marker assisted selection (MAS) analysis is necessary at genomic level allowing rapid and earlier determination of homozygous HO genotypes for sunflower breeding studies. Oleoyl-phosphatidyl choline desaturase (FAD2) has three genes (FAD2-1, FAD2-2 and FAD2-3) in sunflower which has role in the synthesis of linoleic acid from oleic acid (Harwood 1996;Hongtrakul et al 1998;Schuppert et al 2006;Berville et al 2009;Dimitrijevic et al 2017). FAD2-1 has main role in synthesis of linoleic acid, and the mutation in this gene cause increase in oleic acid content in sunflower seeds (Martinez-Rivas et al 2000, 2001Garcia-Diaz et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Yüksek oleik tip ayçiçeğinin güvenilir moleküler seçiminde yeni tasarlanmış primerler ile optimize PCR protokolü

Bilgen

2018

Mediterranean Agricultural Sciences

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High oleic sunflower is one of the most significant oilseed crops due to the stability of its oil in processing and desirable characteristics for health. Determination of high oleic sunflower by standard methods such as gas chromatography is time consuming and expensive. On the other hand, marker-assisted selection analysis with molecular markers associated with high oleic acid trait is a useful and powerful tool in order to facilitate sunflower breeding programs. In this study, we compared three molecular markers which have been used for selection of the high oleic sunflower varieties. We also describe an optimized PCR protocol with newly designed two primer pairs targeting normal sequence of FAD2 gene as internal control and direct analysis of the inserted DNA sequences which is known to be closely linked to the Pervenets mutation. According to results of our study, showing the insertion site which is linked to the Pervenets mutation by the insertion specific PCR protocols is more reliable than the SSR marker for selection of the high oleic sunflower varieties. Because we have not been able to get successful results with the available PCR protocols described for the insertion site, we report here a novel multiplex PCR protocol with newly designed primers enabling reliable discrimination of the high oleic and low oleic sunflower genotypes in a single PCR tube, which offers some advantages to the breeders by means of saving money and time.

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Oleic acid variation and marker-assisted detection of Pervenets mutation in high- and low-oleic sunflower cross

Cited by 32 publications

References 33 publications

Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Peer Review #2 of "Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops (v0.2)"

Yüksek oleik tip ayçiçeğinin güvenilir moleküler seçiminde yeni tasarlanmış primerler ile optimize PCR protokolü

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