Peer Review #2 of "Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops (v0.2)"

Abstract

Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher number of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain Fas. The longest FA detected using GC-FID was a FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has a great potential to be used for the assessment of the FA profile of oil crops.