Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Bueno, Regiane Cristina Oliveira de Freitas; God, Pedro Ivo Vieira Good; Prata, Isadora Oliveira; Pereira, Pedro Henrique Scarpelli; Teixeira, Arlindo Inês; Piovesan, Newton Deniz; Barros, Everaldo Gonçalves de

doi:10.1590/1984-70332018v18n3a37

Cited by 6 publications

(4 citation statements)

References 28 publications

(36 reference statements)

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“…Data presented in Table 2 showed that oleic acid content was significantly (P < 0.05) higher than NRC7 across 5 growing years. Transgressive seggregants were observed for all the three unsaturated fatty acids in all the 5 years, which is in consonance with the earlier report (Bueno et al, 2018). Averaged across 5 years, eight RIL registered oleic acid content in the range of 45-50%, while two RIL were found to contain about 60% oleic acid.…”

Section: Variability For Unsaturated Fatty Acids and Transgressive Sesupporting

confidence: 91%

Genomic Regions Governing the Biosynthesis of Unsaturated Fatty Acids in Recombinant Inbred Lines of Soybean Raised across Multiple Growing Years

Rani

Kumar

Mourya

et al. 2019

J Americ Oil Chem Soc

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The ratio of oleic acid to the combined value of linoleic and α-linolenic acids determines the oxidative stability, and the ratio of linoleic acid to α-linolenic acid is the key to the nutritional value of soybean oil. The present study was conducted to identify genomic regions associated with oleic, linoleic, and α-linolenic acids in recombinant inbred lines (RIL), developed from LSb1 × NRC7, across 5 cropping years. These RIL were genotyped using 105 polymorphic SSR markers across soybean genome and analyzed for fatty acid composition. SSR markers, namely, Satt245 (LGp M), Satt556 (LGpB2), Sat_042 (LGp C1), Staga002 (LGp D1b), Satt684 (LGp A1), and AI856415 (LGpD1b) showed significant (P < 0.05) association with oleic acid for all the 5 years, though this association was weak in the years when the growing temperature during active seed formation stage was high. Quantitative trait loci (QTL) linked to Satt684 (LGp A1), Satt556 (LGp B2), Sat_042 (LGp C1), and AI856415 (LGp D1b) showed pleiotropic influence on the levels of unsaturated fatty acids. Complementation of favorable QTL from LSb1 and NRC7 generated 60% oleic acid and less than 4% α-linolenic acid RIL, stable across 5 cropping years. New SSR markers, namely, Satt245, AI856415, and Staga002 identified to be associated with different unsaturated fatty acids may be useful in improving the efficiency of markerassisted breeding for enhancing the monounsaturated to polyunsaturated fatty acids ratio of soybean oil.

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Section: Variability For Unsaturated Fatty Acids and Transgressive Sesupporting

confidence: 91%

Genomic Regions Governing the Biosynthesis of Unsaturated Fatty Acids in Recombinant Inbred Lines of Soybean Raised across Multiple Growing Years

Rani

Kumar

Mourya

et al. 2019

J Americ Oil Chem Soc

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“…The results showed that maximum and minimum values for all characteristics exceeded the parental values, indicating the occurrence of transgressive segregation. This phenomenon occurs when the parental individuals are divergent for the trait and they do not have the extreme genotypic combinations (Grant 1964), and was observed in other studies with soybean populations segregated by protein content (Hyten et al 2004), grain yield and weight (Mansur et al 1993), sucrose and oligosaccharide content (Kim et al 2006), and oleic (Pham et al 2011, Bueno et al 2018) and linoleic acid contents (Pham et al 2011). Progenies derived from divergent crosses are expected to show a broad spectrum of genetic variability, providing an increase in the number of transgressive segregating individuals (Tyagi andKhan 2010, Mughal et al 2015).…”

Section: Descriptive Statisticsmentioning

confidence: 64%

Effect of a mutation in Raffinose Synthase 2 (GmRS2 ) on soybean quality traits

Silva

Mota

Fonseca

et al. 2019

Crop Breed. Appl. Biotechnol.

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The presence of stachyose and raffinose is considered an antinutritional factor for humans and monogastric animals, leading to limitations on soybean consumption as a protein source. In the present study, the effect of a mutation in the raffinose synthase 2 gene was measured on soybean quality traits. We used an F 2 population with 168 soybean individuals developed by crossing four soybean lines and evaluated oil, protein, sucrose, raffinose, stachyose and fatty acid contents and their relationships. The mutation explained 69.61%, 51.81% and 31.96% of stachyose, raffinose and sucrose variation, respectively, and we were able to produce soybean with average stachyose of 0.18%. The low coefficients of determination for protein and oil indicate that the mutation can be used to increase sucrose and reduce raffinose and stachyose content without major changes in oil and protein.

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“…Therefore, the gel-based marker ABUOP0002 can diagnostically identify corn and rice biotypes of samples that have been taxonomically identified to the species S. frugiperda. The temperature-switch PCR technique used in the current study has been commonly used to assay SNPs in many crop species (Hayden et al 2009;Pagliarani et al 2016;Samad et al 2017;Bueno et al 2018). The technique eliminates the need for sequencing or RFLP profiling for the identification of SNP-based polymorphisms and enables gel-based genotyping as a less expensive alternative.…”

Section: Resultsmentioning

confidence: 99%

Development of a single nucleotide polymorphism-based DNA marker for fall armyworm (Lepidoptera: Noctuidae) biotyping: a case study from the fall armyworm outbreak in Sri Lanka

2020

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Crop damage caused by fall armyworm (FAW), Spodoptera frugipera J.E. Smith (1797) (Lepidoptera: Noctuidae), has generated concern among agriculturists globally. In 2019, FAW was first reported in Sri Lanka, where it caused significant losses to corn crops. However, given that the two FAW biotypes – “rice strain” (R-FAW) and “corn strain” (C-FAW) – are morphologically identical, the biotype(s) present in Sri Lanka were unknown. The current study used the mitochondrial cytochrome c oxidase subunit I gene (mt-CO1) of FAW to biotype nine FAW samples collected in Sri Lanka. The resulting molecular phylogeny revealed that both R-FAW and C-FAW biotypes were present among the samples. In addition, we used the temperature-switch polymerase chain reaction (PCR) technique to develop a gel-based molecular marker. Two fragments were successfully amplified by the newly developed marker ABUOP0002, with fragment sizes of 341 bp from R-FAW and 204 bp from C-FAW. This demonstrates that ABUOP0002 can serve as a diagnostic gel-based molecular marker to identify the R-FAW and C-FAW biotypes from samples already identified to species level as S. frugiperda through taxonomical keys and provides a possible alternative to more expensive sequencing-based assays.

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Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping

Cited by 6 publications

References 28 publications

Genomic Regions Governing the Biosynthesis of Unsaturated Fatty Acids in Recombinant Inbred Lines of Soybean Raised across Multiple Growing Years

Genomic Regions Governing the Biosynthesis of Unsaturated Fatty Acids in Recombinant Inbred Lines of Soybean Raised across Multiple Growing Years

Effect of a mutation in Raffinose Synthase 2 (GmRS2 ) on soybean quality traits

Development of a single nucleotide polymorphism-based DNA marker for fall armyworm (Lepidoptera: Noctuidae) biotyping: a case study from the fall armyworm outbreak in Sri Lanka

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