In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Bakhtiar, Ziba; Mirjalili, Mohammad Hossein; Sonboli, Ali

doi:10.1590/1984-70332016v16n1a8

Cited by 50 publications

(38 citation statements)

References 22 publications

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“…However the shoot -tips which were cultured on NN and B5media produced the lowest number of shoots, shoot length, number of leaves and number of leaves/shoot. These funding are harmony with Arafeh et al, (2003), Yildirim (2013, El-Beyrouthy et al, (2015) and Bakhtiar et al,(2016) they found that the MS was the best medium with shoot-tip on Lamiaceae family plants. Table, 4and Fig 1 show significant effects for cytokinin types on all studied traits.…”

Section: Effect Of Explant Typesupporting

confidence: 86%

Micropropagation of Oregano (Origanum syriacum L.) Through Tissue Culture Technique

Abdallah

Yakoup

Abdalla

2017

Journal of Plant Production

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This research was carried out at the Plant Tissue Culture Laboratory (PTCL) in Faculty of Environmental Agricultural Sciences, Arish University, Egypt during 2013 to 2016. The aim of this study was using tissue culture technique for the micropropagation of Origanum syriacum L. plant. One node cutting and shoot tip were cultured on Murashige and Skoog (MS), Nitsch and Nitsch (NN) and Gamborg (B5) media. The results showed that shoot tip cultured on the MS medium had the best combination for the establishment stage of mother plants. Shoots cultured on MS medium supplemented with0.50 mgl -1 Kin and 0.05 mgl -1 NAA produced the highest shoots developed during multiplication stage. MS medium supplemented with 0.40 mgl -1 thiamine achieved the best shoots development compared with the other additive treatments and control. Addition of 1 mgl -1 IBA to the full strength MS medium and 1.00 mgl -1 IAA to the half strength MS had the highest number of main roots and root lengths. Hardening the produced rooted plantlets were done in a greenhouse in pots containing mixture of peatmoss, vermiculite and washed sand at the rate (1:1:1) and after adjustment of mixture pH. Plantlets were successfully acclimated with 93 % survive.

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Section: Effect Of Explant Typesupporting

confidence: 86%

Micropropagation of Oregano (Origanum syriacum L.) Through Tissue Culture Technique

Abdallah

Yakoup

Abdalla

2017

Journal of Plant Production

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“…The callus formation did not differ during the cultivation, presenting values close to 36%. The occurrence of callus during in vitro cultivation has been reported for many Lamiacea especies (Meftahizade et al, 2010;Bakhtiar et al, 2016;Monfort et al, 2018), demonstrating a type of responsiveness to the medium.…”

Section: Salt Concentration In the Ms Mediummentioning

confidence: 85%

“…Probably, there is an influence of factors related to callus formation, mainly growth regulators and activators of developmental genes. As previously discussed, callus formation in Ocimum is probably associated with the presence of growth regulators in the culture medium (Bakhtiar et al, 2016). It is not clear yet the way in which activated carbon promotes the growth of seedlings.…”

Section: Interaction Of Sucrose and Activated Carbonmentioning

confidence: 94%

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Trettel¹,

Nascimento²,

Barbosa³

et al. 2018

JAS

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Genovese basil has great economic potential; however, there is no established micropropagation protocol for this species. Therefore, this study aimed at assessing the in vitro growth of Genovese basil in response to different concentrations of salts in the Murashige and Skoog medium (MS) and interaction of sucrose and activated carbon. Two assays were conducted independently in an in vitro environment using the MS medium, regulators, agar, and five salt concentrations (0, 25, 50, 70 and 100%). In the second assay, two concentrations of sucrose (30 and 60 g L ) were tested.In addition, copper and zinc were quantified in the roots. The results showed that shoots were favored when the medium was at its full strength (100% salts), with seedlings forming more leaves.This result may be associated with a higher demand for nitrogen and because of the ionic balance between NH 4 + and NO 3 -. High concentrations of salts affected the roots, but a reduction to 70% salt favored root development. Doubling the usual dose of sucrose (60 g L -1 ) damaged the growth of the seedlings. Damage caused by osmotic and oxidative potentials, and by toxic compounds may be related to the observed results. The amount of copper and zinc in the root increased with increased concentrations of activated carbon in the medium. The presence of activated carbon reduced callus formation but did not mitigate the effects of increased sucrose concentration.

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“…Other application of molecular markers in this genus is the analysis of the reliability of the in vitro propagation regarding the genetic homogeneity, most of times associated to the phytochemical productivity of the produced plantlets, as the experiment reported by Bakhtiar et al [48] using RAPDs. In this work RAPD profiles confirmed the homogeneity and highperformance liquid chromatography (HPLC) confirmed the phytochemical productivity of the in vitro regenerated plants.…”

Section: Genetic Analysismentioning

confidence: 99%

Thymus Plants: A Review—Micropropagation, Molecular and Antifungal Activity

Leal¹,

Taghouti²,

Nunes³

et al. 2017

Active Ingredients From Aromatic and Medicinal Plants

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Medicinal and aromatic plants are important sources for plant secondary metabolites. The genetic manipulation of plants associated with in vitro plant regeneration systems facilitates efforts to engineer secondary product metabolic pathways. The fungal infections have been increasing in recent years due to several factors, namely, the increased incidence of high-risk patients, particularly immunocompromised hosts. Aromatic plants have been empirically used as antimicrobial agents, but the mechanisms of action are still unknown. Thyme has a great interest due to the possibility of its use in different applications, in medicine, in the cosmetic industry, or as food additives. Several studies have shown that thyme oils possess antimicrobial activity. Increasingly, plant breeding has taken advantage of molecular biology developments in order to genotype the species of interest to accelerate their selection. These approaches consist in choosing desired genotypes based on molecular markers or the knowledge of the genes involved in a particular trait. The in vitro culture techniques can be used to multiply plants selected after molecular and antifungal studies. The course of the investigation and the current state in relation to micropropagation, molecular studies, and antifungal action of the Thymus genus plants will be presented.

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In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

Abstract: -This is the first attempt towards an efficient regeneration protocol for an endangered and valuable medicinal plant,

Cited by 50 publications

References 22 publications

Micropropagation of Oregano (Origanum syriacum L.) Through Tissue Culture Technique

Micropropagation of Oregano (Origanum syriacum L.) Through Tissue Culture Technique

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Thymus Plants: A Review—Micropropagation, Molecular and Antifungal Activity

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