Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract

Abstract

The effects of reduced doses of Ovaprim ™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at ¼ of the recommended dose can successfully replace cPE.O efeito de doses reduzidas de Ovaprim ® (GnRHa + domperidona) na liberação do sêmen de Brycon orbignyanus e Prochilodus lineatus foi avaliado. Além disso, a qualidade do sêmen foi comparada entre as amostras frescas, equilibradas e descongeladas. Os machos receberam dose única e reduzida de Ovaprim ® (0,125 ou 0,25 ml/kg); os machos-controle receberam extrato de hipófise (cPE; 3 mg/kg). O sêmen fresco foi avaliado quanto ao volume, concentração, e osmolalidade e pH do plasma seminal. Em seguida, o sêmen foi diluído num meio de congelamento, equilibrado por 15-20 min e congelado em botijão de vapor de nitrogênio (dry-shipper). A motilidade espermática foi analisada durante 60 s pós-ativação no sêmen fresco, equilibrado e descongelado. A qualidade do sêmen não diferiu entre os machos tratados com Ovaprim ® (ambas as doses) ou cPE, assim foi feito um pool desses dados. Em B. orbignyanus, a motilidade foi maior no sêmen fresco (99%) do que no equilibrado (81%); a motilidade do sêmen descongelado caiu para 42%. Em P. lineatus, a motilidade foi semelhante entre o sêmen fresco (99%) e equilibrado (92%); a motilidade do sêmen descongelado foi 73%. A motilidade caiu em função do tempo pós-ativação, e essa queda foi significante após 60 s no sêmen fresco e equilibrado, e tão precoce quanto 30 s no sêmen descongelado, em ambas as espécies. Ovaprim ® a ¼ da dose recomendada pode substituir o cPE com sucesso.

Extender composition and osmolality affects post-thaw motility and velocities of piracanjuba Brycon orbignyanus (Valenciennes, 1850) (Characiformes) sperm

“…Fresh sperm quality of B. orbignyanus was assessed after hormonal treatment with carp pituitary extract. The values observed for sperm quality were all within the range observed for fresh sperm of this species (Nascimento et al., ; Gonçalves et al., ; Viveiros et al., ). It is interesting to observe that the sperm of this species is characterized by a large volume, frequently above 10 ml, and a low concentration, mostly below 10 × 10 9 sperm ml −1 (Viveiros and Godinho, ).…”

“…After a few weeks, straws were thawed in a waterbath (Water‐bath MA 127, Marconi, Brazil) at 60°C for 3 s, and post‐thaw sperm quality was immediately estimated using the CASA system, following the methodology described in Viveiros et al. (). Briefly, post‐thaw sperm was activated in a Makler ™ counting chamber (Sefi‐Medical Instruments ltd, Haifa, Israel) placed under a phase contrast microscope (Eclipse E200; Nikon, Tokyo, Japan) at 100× magnification with a green filter and pH 1 position.…”

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

“…Sperm samples were stored at 4°C for 48 hr and diluted in 5% BTS™ (80% glucose, 12.7% sodium citrate, 2.7% EDTA, 2.7% NaHCO 3 , 1.5% KCl, 0.5% gentamycin sulphate; Beltsville Thawing Solution Minitüb™) and 10% DMSO (dimethyl sulfoxide) at a 1:4 ratio as recommended by Miliorini et al (2011) and Murgas et al (2007). Sperm was equilibrated for 15-20 min (Viveiros, Gonçalves, Nascimento, & Leal, 2015), drawn into sealed 0.5-ml straws, in triplicate, identified and maintained in a freezing equipment (IceCube® 14S) programmed with the cooling rates adapted from Gaitán-Espitia, Martínez-Silva, Borrero, Ramírez, and Valencia (2013) as follows: a slow cooling rate of 15°C/min (CR15) and a fast cooling rate of 30°C/ min (CR30) ( Table 1)…”

Criopreservación de semen de bagre rayado Pseudoplatystoma magdaleniatum con tres diferentes crioprotectores

“…Six semen straws were obtained per treatment and each one was evaluated in triplicate. After reaching equilibrium (10 minutes) the straws were frozen in a dry thermos with nitrogen vapors (MVE 4/2V, AL, USA) for 30 minutes and then transferred to a storage thermos (MVE 24/12V, AL, USA), submerging them directly in liquid nitrogen (Cruz-Casallas et al, 2006;Viveiros et al, 2015). The cryopreserved semen straws were thawed by direct immersion in a serological water bath (Memmert, WNB 7-45, Germany) at 35 °C for 90 seconds (Atencio-García et al, 2017).…”