Extender composition and osmolality affects post-thaw motility and velocities of piracanjuba Brycon orbignyanus (Valenciennes, 1850) (Characiformes) sperm

Abstract

Summary The aim of this study was to evaluate the effects of extender composition and osmolality on post‐thaw motility of Brycon orbignyanus sperm. Eight extenders comprising combinations of two compositions (NaCl and glucose) and four osmolalities (285, 325, 365 and 405 mOsm kg−1) were tested. Methyl glycol was used as cryoprotectant. Diluted sperm was loaded into 0.25 ml straws, frozen in a nitrogen vapor vessel (dry shipper) and stored in a liquid nitrogen vessel. Straws were thawed in a water bath at 60°C for 3 s and sperm was immediately evaluated for motility rate and velocities (curvilinear = VCL; straight line = VSL; average path = VAP). Seminal plasma osmolality was also determined (249 mOsm kg−1). Both extender composition and osmolality affected post‐thaw sperm motility. In general, sperm cryopreserved in NaCl was of better motility than that in glucose, and at lower osmolalities better than at higher ones. High post‐thaw quality, with motility above 60% and VCL above 140 μm s−1, was observed only in samples frozen in NaCl at 285 mOsm kg−1. Different from most of the sperm from freshwater species that need a cell membrane protector (such as sugars), B. orbignyanus sperm should be frozen in an ionic solution for better protection during freezing and thawing processes. Furthermore, this solution should be prepared at an osmolality just above seminal plasma osmolality. Cryopreserved sperm can be used both for aquaculture purposes and for conservational programs, since B. orbignyanus is a threatened species.

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

“…CASA development Acipenser ruthenus sterlet (Boryshpolets et al, 2013) Anguilla anguilla European eel (Gallego et al, 2013b) Cyprinus carpio common carp (Boryshpolets et al, 2013) Danio rerio zebrafish (Wilson-Leedy et al, 2009) Oncorhynchus mykiss rainbow trout (Boryshpolets et al, 2013) Sperm cryopreservation Acipenser baerii Siberian sturgeon (Judycka et al, 2015;Sieczynski et al, 2015) Anguilla anguilla European eel (Asturiano et al, 2004(Asturiano et al, , 2007 Brycon orbignyanus piracanjuba (López et al, 2015) Clarias gariepinus African catfish (Rurangwa et al, 2001) Cyprinus carpio common carp (Warnecke & Pluta, 2003) Dicentrarchus labrax European seabass (Cabrita et al, 2011) Gadus morhua Atlantic cod (Butts et al, 2011a) Perca fluviatilis Eurasian perch (Bernáth et al, 2015) Salmo salar Atlantic salmon (Dziewulska et al, 2011) Sparus aurata gilthead seabream (Beirão et al, 2011b;Cabrita et al, 2010) Thymallus thymallus grayling (Horváth et al, 2015) Sperm chilled storage Anguilla anguilla European eel (Peñaranda et al, 2010c) Hippoglossus hippoglossus Atlantic halibut (Babiak et al, 2006) Oncorhynchus mykiss rainbow trout (Lahnsteiner et al, 2004) Salmo trutta brown trout (Formicki et al, 2015) Steindachneridion parahybae surubim-do-Paraíba (Sanches et al, 2015) Activation media tests Coregonus lavaretus European whitefish (Dziewulska et al, 2015) Cyprinus carpio common carp (Zarski et al, 2015) Oncorhynchus mykiss steelhead (Kanuga et al, 2012) Perca fluviatilis Eurasian perch (Lahnsteiner, 2011) Broodstock management Dicentrarchus labrax European seabass (Felip et al, 2006) Solea senegalensis Senegalese sole (Beirão et al, 2011a) Spermiation induction Acipenser ruthenus sterlet (Alavi et al, 2012) Anguilla anguilla European eel ...…”

Seminal plasma features of Prochilodus lineatus and Brycon orbignyanus throughout two consecutives spawning seasons

“…Seminal plasma osmolality ranged from 276 to 346 mOsm/kg (Gonçalves, Nascimento, Costa, Leal, & Viveiros, ) and from 220 to 252 mOsm/kg (Viveiros, Taffarel, & Leal, ) in P. lineatus , and from 289 to 313 mOsm/kg (Gonçalves et al, ) and from 216 to 275 mOsm/kg (Viveiros et al, ) in B. orbignyanys . Seminal plasma pH values reported varied from 8.22 to 8.77 in P. lineatus (Viveiros et al, ) and from 7.88 to 8.21 in B. orbignyanus (Lopez, Leal, & Viveiros, ).…”

Criopreservação de sêmen de jundiá amazônico (Leiarius marmoratus) e pintado (Pseudoplatystoma corruscans)

“…Diferente da maioria dos peixes de água doce, que necessitam de um açúcar na composição do diluidor, o sêmen de piracanjuba (Brycon orbignyanus) apresentou melhores resultados com o uso de soluções salinas (LÓPEZ et al, 2015). Em salmonídeos, um estudo avaliou a substituição da glicose por outros açúcares como a trealose e a sacarose, e os efeitos positivos na motilidade foram espécie específicos (NYNCA et al, 2016).…”

“…A utilização do sistema CASA em peixes nativos, como o tambaqui (Colossoma macropomum), curimba (Prochilodus lineatus), piracanjuba (Brycon orbignyanus) e jundiá cinza (Rhandia quelen), está tornando-se mais comum (LÓPEZ et al, 2015;VARELA et al, 2015;GOES et al, 2017). No entanto, na avaliação do sêmen de jundiá amazônico (Leiarius marmoratus) e do pintado (Pseudoplatystoma corruscans), o sistema CASA foi pouco utilizado e as informações sobre os parâmetros de configuração são pequenas (MEDINA ROBLES et al, 2008;VELAVERDE, 2013;.…”