Nanostructured Hydroxyapatite from Hen´s Eggshells Using Sucrose as a Template

Horta, Marla Karolyne dos Santos; Moura, F.; Aguilar, Marilza Sampaio; Westin, Cecília Buzatto; Campos, José Brant de; Peripolli, S.; Ramos, Vitor Santos; Navarro, María Isabel Rubio; Archanjo, Bráulio S.

doi:10.1590/1980-5373-mr-2020-0266

Cited by 8 publications

(12 citation statements)

References 59 publications

(66 reference statements)

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“…Out of 71 relevant article titles collected from SCOPUS, PubMed, and Google Scholar, only 36 were included after screening the title, abstract, and content for in vitro degradation and cytotoxicity testing for the biocompatibility of eggshell-based hydroxyapatite. The articles came from several countries, such as India [ 11 , 14 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ], Bangladesh [ 41 , 42 ], China [ 43 ], Korea [ 25 , 44 , 45 ], Turkey [ 46 , 47 , 48 ], Thailand [ 49 , 50 ], Brazil [ 26 ], Italy [ 51 ], USA [ 52 ], Romania [ 2 ], Vietnam [ 53 , 54 ], Colombia [ 55 ], and Egypt [ 56 ]. The study selection of the articles included in the systematic review is shown in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…The cytotoxicity test, one of the biological evaluation and screening tests, uses tissue cells in vitro to observe the cell growth, reproduction, and morphological effects of medical devices [ 25 ]. The in vitro cytotoxicity test uses ISO 10993-5:2009 [ 26 ]. In this work, the limitation of cytotoxicity tests was based on the direct contact test.…”

Section: Resultsmentioning

confidence: 99%

“…The statistical significance of the difference between the control and treatment groups was evaluated using a one-way analysis of variance (ANOVA) [ 45 ]. The quantification of viable cells was evaluated with the help of fluorescence microscopy [ 2 , 25 , 26 ], field emission scanning electron microscopy (FESEM) [ 52 ], and scanning electron microscopy [ 55 ]. The optical density test results from articles [ 2 , 20 , 49 , 50 ] are displayed in Figure 4 with a comparison of the control samples and treatment material variations, which can be used to determine the value of cell viability.…”

Section: Resultsmentioning

confidence: 99%

“…Biomaterials should not release any toxins or produce harmful reactions in the human body. Cytotoxicity is related to the degradation process of biomaterials, which can stimulate or inhibit the metabolism of cultured stem cells [ 26 ]; biomaterial degradation is affected by porous size and porous interconnectivity [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Kumar et al performed a cytotoxicity test on hydroxyapatite using mouse fibroblast 3T3-L1 cells with variable observation times [ 20 ]. Horta et al investigated eggshell-based hydroxyapatite cells with 98.9% viability that were cultured in dental pulp stem cells (DPSCs) for 24 h [ 26 ]. Both in vitro degradation and cytotoxicity tests were carried out to determine the biocompatibility of the materials.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Degradation and Cytotoxicity of Eggshell-Based Hydroxyapatite: A Systematic Review and Meta-Analysis

et al. 2021

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Objective: This review focuses on the in vitro degradation of eggshell-based hydroxyapatite for analyzing the weight loss of hydroxyapatite when applied in the human body. Cytotoxicity tests were used to observe cell growth and morphological effects. A systematic review and meta-analysis were conducted to observe the weight loss and viable cells of hydroxyapatite when used for implants. Method: Based on the Population, Intervention, Comparison, and Outcome (PICO) strategy, the articles used for literature review were published in English on SCOPUS, PubMed, and Google Scholar from 1 January 2012 to 22 May 2021. Data regarding existing experiments in the literature articles the in vitro degradation and cytotoxicity testing of eggshell-based hydroxyapatite determined the biocompatibility of the materials. A meta-analysis was conducted to calculate the mean difference between the solutions and soaking times used for degradation and the stem cells used for cytotoxicity. Results: From 231 relevant studies, 71 were chosen for full-text analysis, out of which 33 articles met the inclusion criteria for degradation and cytotoxicity analysis. A manual search of the field of study resulted in three additional articles. Thus, 36 articles were included in this systematic review. Significance: The aim of this study was to highlight the importance of the biocompatibility of eggshell-based hydroxyapatite. The weight loss and viability cells of eggshell-based hydroxyapatite showed optimum results for viable cells requirements above 70%, and there is a weight loss of eggshell-based hydroxyapatite for a material implant. The meta-analysis indicated significant differences in the weight loss of eggshell-based hydroxyapatite materials with different soaking times and solutions used. The various kinds of stem cells for incubation of cultured cells in contact with a device, either directly or through diffusions with various kinds of stem cells from animals and humans, yielded viability cells above 70%.

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