Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion

Guastaldi, Fernando Pozzi Semeghini; Foggi, Camila Cristina de; Santana, Luís Carlos Leal; Vaz, Luís Geraldo; Vergani, Carlos Eduardo; Guastaldi, Antônio Carlos

doi:10.1590/1980-5373-mr-2019-0012

Cited by 6 publications

(7 citation statements)

References 55 publications

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“…In the present study, from the FEG-SEM analysis, it was possible verify that the laser irradiation and HA deposition to both cpTi and Ti-15Mo alloy groups, produced a unique 3D surface topography, in accordance to that found for cpTi obtained by Braga et al [38], Heinrich et al [39] and Queiroz et al [18] and for Ti-15Mo alloy by Oliveira et al [12], Guastaldi et al [40] and Pires et al [13].…”

Section: Discussionsupporting

confidence: 89%

“…Regarding the results of the contact angle assay, a complete wetting for the laser and HA deposition groups was shown. Similar results were observed by Oliveira et al [ 12 , 41 ], Guastaldi et al [ 40 ] and Pires et al [ 13 ] after laser irradiation on Ti-15Mo surface and Queiroz et al [ 18 ] after laser irradiation and HA deposition on cpTi surface. It is known that osteoblast culture exhibits better adhesion and growth on hydrophilic surfaces [ 45 ] and wettability influences the osteoblastic response in vitro [ 46 ].…”

Section: Discussionsupporting

confidence: 87%

“…From the EDX analysis, it was possible to prove that no contamination was found after laser irradiation and HA deposition to both cpTi and Ti-15Mo alloy groups. Similar findings were observed by Bini et al [ 15 ] and Filho et al [ 16 ] after laser irradiation on cpTi surface, Oliveira et al [ 12 , 41 ], Guastaldi et al [ 40 ] and Pires et al [ 13 ] after laser irradiation on Ti-15Mo alloy surface and Queiroz et al [ 18 ] after laser irradiation and HA deposition on cpTi surface. In addition, using X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analysis, it has already been shown the phases and the chemical composition of titanium samples (cpTi and Ti-15Mo) after laser surface modification and deposition of calcium phosphates using the same methodology described in this manuscript (Bini et al [ 15 ]; Filho et al [ 16 ]; Santos et al [ 42 – 44 ]).…”

Section: Discussionsupporting

confidence: 83%

“…The literature reports several studies that employs the technology of laser modification of cpTi and Ti alloys, with improvements in their physicochemical properties, wear and corrosion resistance, favoring the in vivo bone response to these surfaces [ 12 , 13 , 15 , 16 , 18 , 40 ]. To our knowledge this is the first study that combines laser surface modification of this new Ti-15Mo alloy and HA deposition on the behavior of osteoblast-like cells.…”

Section: Discussionmentioning

confidence: 99%

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Behavior of rat bone marrow stem cells on titanium surfaces modified by laser-beam and deposition of calcium phosphate

Florian

Guastaldi

Cominotte

et al. 2021

J Mater Sci: Mater Med

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Objectives The aim of this study was to evaluate the behavior of rat bone marrow stem cells seeded on a Ti-15Mo alloy surface modified by laser-beam irradiation followed by calcium phosphate deposition. Materials and methods A total of four groups were evaluated: polished commercially pure titanium (cpTi): Ti-P; laser irradiation + calcium phosphate deposition on cpTi: Ti-LCP; polished Ti-15Mo alloy: Ti15Mo-P; and laser irradiation + calcium phosphate deposition on Ti-15Mo alloy: Ti15Mo-LCP. Before and after laser irradiation and calcium phosphate deposition on the surfaces, physicochemical and morphological analyses were performed: Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDX). The wettability of the samples was evaluated by contact angle measurement. In addition, the behavior of osteoblast-like cells to these surfaces was evaluated for cell morphology, adhesion, proliferation and viability, evaluation of alkaline phosphatase formation and gene expression of osteogenesis markers. Results Surfaces wet-abrade with grit paper (P) showed oriented groves, while the laser irradiation and calcium phosphate deposition (LCP) produced porosity on both cpTi and Ti15Mo alloy groups with deposits of hydroxyapatite (HA) crystals (SEM). EDX showed no contamination after surface modification in both metal samples. A complete wetting was observed for both LCP groups, whereas P surfaces exhibited high degree of hydrophobicity. There was a statistical difference in the intragroup comparison of proliferation and viability (p < 0.05). The ALP activity showed higher values in the Ti15Mo alloy at 10 days of culture. The gene expression of bone related molecules did not present significant differences at 7 and 14 days among different metals and surface treatments. Conclusion Ti15-Mo seems to be an alternative alloy to cpTi for dental implants. Surface treatment by laser irradiation followed by phosphate deposition seems to positively interact with bone cells. Clinical relevance Ti-15Mo alloy surface modified by laser-beam irradiation followed by calcium phosphate deposition may improve and accelerate the osseointegration process of dental implants.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Behavior of rat bone marrow stem cells on titanium surfaces modified by laser-beam and deposition of calcium phosphate

Florian

Guastaldi

Cominotte

et al. 2021

J Mater Sci: Mater Med

Self Cite

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“…Besides, the titanium-6aluminum-4vanadium (Ti-6Al-4V) alloy shows better wettability performance compared to other Ti-based materials (Ti-6Al-2Zr-1Mo-1V, Ti-15Mo-3Al-2.7Nb-0.2Si) [26]. At last, previous studies stressed the concern about the dissolution of ions and, consequently, the inherent cytotoxicity provided by these alloys when in contact with the physiological environment [27,28]. In this concern, the release of aluminum (Al) and vanadium (V) ions was referred to be potentially toxic to the peri-implant surrounding cells since it triggers the inflammatory response in the peri-implant tissues [29].…”

Section: Introductionmentioning

confidence: 99%

Strontium-loaded titanium-15molybdenum surface improves physicochemical and biological properties in vitro

Matos¹,

Santana²,

Cominotte³

et al. 2022

Biomed. Phys. Eng. Express

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The titanium alloy composition and microdesign affect the dynamic interplay between the bone cells and titanium surface in the osseointegration process. The current study aimed to evaluate the surface physicochemical properties, electrochemical stability, and the metabolic response of the MC3T3-E1 cells (pre-osteoblast cell line) cultured onto titanium-15molybdenum (Ti-15Mo) discs treated with phosphoric acid (H3PO4) and sodium hydroxide (NaOH) and/or strontium-loading by the hydrothermal method. The X-ray dispersive energy spectroscopy (EDS) and X-ray diffraction (XRD) analysis showed no trace of impurities and the possible formation of hydrated strontium oxide (H2O2Sr), respectively. The confocal laser microscopy (CLSM) analysis indicated that titanium samples treated with strontium (Sr) showed greater surface roughness. The acid/alkali treatment prior to the hydrothermal Sr deposition improved the surface free energy and resistance to corrosion of the Ti-15Mo alloy. The acid/alkali treatment also provided greater retention of the Sr particles on the Ti-15Mo surfaces accordingly with inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The AlamarBlue and fluorescence analysis indicated noncytotoxic effects against the MC3T3-E1 cells, which allowed cells adhesion and proliferation, with greater cells' spreading in the Sr-loaded Ti-15Mo samples. These findings suggest that Sr deposition by the hydrothermal method has the potential to enhance the physicochemical properties of the Ti-15Mo previously etched with H3PO4 and NaOH, and also improve the initial events related to cell-mediated bone deposition.

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LASER as a tool for surface modification of dental biomaterials: A review

Saran

Ginjupalli

George

et al. 2023

Heliyon

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Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion

Cited by 6 publications

References 55 publications

Behavior of rat bone marrow stem cells on titanium surfaces modified by laser-beam and deposition of calcium phosphate

Behavior of rat bone marrow stem cells on titanium surfaces modified by laser-beam and deposition of calcium phosphate

Strontium-loaded titanium-15molybdenum surface improves physicochemical and biological properties in vitro

LASER as a tool for surface modification of dental biomaterials: A review

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