Strontium-loaded titanium-15molybdenum surface improves physicochemical and biological properties in vitro

Matos, Flávia Gomes; Santana, Luís Carlos Leal; Cominotte, Mariana Aline; Silva, Fernando Santos da; Vaz, Luís Geraldo; Oliveira, Diego Pedreira de; Cirelli, Joni Augusto

doi:10.1088/2057-1976/ac71cf

Cited by 7 publications

(3 citation statements)

References 74 publications

(89 reference statements)

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“…In summary, these results are consistent with similar literature that have demonstrated the stimulating effect of Sr on osteoblastic cell behavior [16,53]. The potential for improved bone formation by incorporating Sr into biomaterials has also been well-documented in in vitro and in vivo studies [23,[54][55][56][57]. However, some limitations should be noted in this study.…”

Section: Shi Et Al (2017)supporting

confidence: 91%

Titanium micro-nano texturized surface with strontium incorporation improves osseointegration. An in vivo and in vitro study.

Filho,

Marcantonio,

Oliveira

et al. 2023

Preprint

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Objectives: This study aimed to investigate the osseointegration of titanium (Ti) implants with micro-nanotextured surfaces functionalized with strontium additions (Sr) in a pre-clinical rat tibia model. Materials and Methods: Ti commercially pure (cp-Ti) implants were installed bilaterally on the tibia of 64 Holtzman rats, divided into four experimental groups (n=16/group): (1) Machined surface - control (C group); (2) Micro-nano textured surface treatment (MN group); (3) Micro-nano textured surface with Sr2+ addition (group MNSr); (4) Micro-nano textured surface with a higher complementary addition of Sr2+ (MNSr+). Two experimental euthanasia periods were assessed at 15 and 45 days (n=8/period). The tibia was subjected to micro-computed tomography (μ-CT), histomorphometry with the EXAKT system, removal torque (TR) testing, and gene expression analysis by PCR-Array of 84 osteogenic markers. Gene expression and protein production of bone markers were performed in an in vitro model with MC3T3-E1 cells. The surface characteristics of the implants were evaluated by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and laser scanning confocal microscopy. Results: SEM, confocal, and EDS analyses demonstrated the formation of uniform micro-nano textured surfaces in the MN group and Sr addition in the MNSr and MNSr+ groups. The removal torque (TR) test indicated greater osseointegration in the later period for treated surfaces. Histological analysis highlighted the benefits of the treatments, especially in cortical bone, where there was an increase in bone-implant contact in groups MN (15 days) and MNSr (45 days) compared to the control group. Micro-CT revealed similar trends to histological analyses, although they did not reach statistical significance between the treatment groups and the control group. Gene expression analysis of osteogenic activity markers showed modulation of various genes related to osteogenesis. According to the in vitro model, RT-qPCR and ELISA demonstrated that the treatments favored gene expression and production of osteoblastic differentiation and activity markers. Conclusions: Our results demonstrated that the micro-nano textured surface and Sr addition may be effective in improving and accelerating the implant osseointegration in cortical and cancellous bone, resulting in increased osseointegration. This makes this approach an attractive option for modifying titanium implant surfaces with significant potential in clinical practice. Clinical relevance: Subsequent advancements in the design and composition of dental implant surfaces are deemed pivotal to enhance osseointegration. This is a pre-clinical study in an animal model that evaluated the behavior of new strontium-doped surfaces. The results showed that doped titanium surfaces with Sr can improve osseointegration events, highlighting the potential use of Sr surfaces to improve results in complex clinical scenarios.

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Section: Shi Et Al (2017)supporting

confidence: 91%

Titanium micro-nano texturized surface with strontium incorporation improves osseointegration. An in vivo and in vitro study.

Filho,

Marcantonio,

Oliveira

et al. 2023

Preprint

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“…Acid etching: 18% HCl and 48% H 2 SO 4 were mixed in a volume ratio V:V = 1:1, and then the samples were put into the mixed solution for 30 min at 25 °C. Alkali etching: the samples were put into the sodium hydroxide aqueous solution with a concentration of 5 mol/L for 24 h at 60 °C (Matos et al, 2022).…”

Section: Surface Modificationmentioning

confidence: 99%

Surface modification improving the biological activity and osteogenic ability of 3D printing porous dental implants

Qin

Gao

et al. 2023

Front. Mater.

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Objective: To explore the mechanical properties, biological activity, and osteogenic ability of 3D printed TC4 titanium (Ti) alloy dental implants treated with surface modification.Methods: Dental implants with 30% porosity were manufactured using selective laser melting (SLM) technology (group 3D), while traditional numerically-controlled machine tools (CNC) were used to manufacture implants without porosity (group SL). The implants were then surface modified through sandblasting and acid etching (groups 3DA1 and SLA1), and then alkali etching (groups 3DA2 and SLA2). The physicochemical properties of the implants were measured using a Vickers hardness instrument, scanning electron microscope (SEM), X-ray diffraction (XRD), energy dispersive spectrometer (EDS), and profilograph before and after surface modification. Next, the biocompatibility, bioactivity, and osteogenic ability of the implants were evaluated using apatite deposition experiments, alkaline phosphatase (ALP) activity, and semiquantitative analysis of extracellular matrix mineralization.Results: There were significant differences in morphology, geometric accuracy, mechanical properties, surface roughness, and hydrophilicity between groups 3D and SL. Furthermore, surface modification improved the physicochemical properties of the porous implants. Implants with sandblasting, acid etching, and alkali etching demonstrated better biocompatibility, bioactivity, and osteogenic ability than implants without surface modification in both groups 3D and SL. Additionally, the implants of groups 3D have higher bioactivity than that of groups SL.Conclusion: Surface modification and the macroporous structure of implants can improve their bioactivity and osteogenic ability, enhancing the application of Ti alloy dental implants.

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“…Several studies have been conducted on the influence of surface modifications osseointegration (Albrektsson et al, 1981;Smeets et al, 2016). Thus, the physical-chemical properties of implants such as wettability, contact angle, roughness, chemical composition are some characteristics that have been modified through surface treatments such as laser irradiation (Hallgren et al, 2003), acid treatment and alkaline etching (Matos et al, 2022;Oliveira et al, 2014), anodization (Le Guehennec et al, 2007) in order to optimize osseointegration.…”

Section: Introductionmentioning

confidence: 99%

Adherence of Escherichia coli and blood elements in titanium discs submitted to anodic oxidation. In vitro study

Matos

Albach

Bermudez

et al. 2022

RSD

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Modifications of implants by means of surface treatments are performed to optimize the biochemical interactions of the bone deposition process. However, if on the one hand they favor the adhesion of blood elements, on the other hand they can also enable the formation of biofilm. This study evaluated the adherence of blood cells and Escherichia coli in titanium discs submitted to surface treatments by anodic oxidation (OA), sandblasting followed by acid etching (JAT) compared with untreated discs (Li). To evaluate the adherence of microorganisms were performed: atomic force microscopy; Field Emission Gun Scanning Electron Microscopy (FEG-SEM); biofilm formation was evaluated by spectrophotometer turbidity analysis and colony forming unit (CFU/mL) before and after simulated brushing. For blood cell adherence, the blood collected from a patient was deposited and fixed on the discs and analyzed in the FEG-SEM being classified according to the "Blood Element Adherence Index". The results showed a slight increase in the adhesion of microorganisms in samples treated by anodic oxidation. However, the microorganisms were distributed singly and not in conglomerates, with no biofilm formation unlike the Li group. Regarding the adherence of blood elements, the Li group showed higher adherence and lower amount was found in the JAT group, but there was no statistically significant difference between the groups. The results of this study suggest that anodic oxidation treatment may favor the adherence of blood cells and fibrin mesh, contributing to the early stages of bone deposition.

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Strontium-loaded titanium-15molybdenum surface improves physicochemical and biological properties in vitro

Cited by 7 publications

References 74 publications

Titanium micro-nano texturized surface with strontium incorporation improves osseointegration. An in vivo and in vitro study.

Titanium micro-nano texturized surface with strontium incorporation improves osseointegration. An in vivo and in vitro study.

Surface modification improving the biological activity and osteogenic ability of 3D printing porous dental implants

Adherence of Escherichia coli and blood elements in titanium discs submitted to anodic oxidation. In vitro study

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