The titanium alloy composition and microdesign affect the dynamic interplay between the bone cells and titanium surface in the osseointegration process. The current study aimed to evaluate the surface physicochemical properties, electrochemical stability, and the metabolic response of the MC3T3-E1 cells (pre-osteoblast cell line) cultured onto titanium-15molybdenum (Ti-15Mo) discs treated with phosphoric acid (H3PO4) and sodium hydroxide (NaOH) and/or strontium-loading by the hydrothermal method. The X-ray dispersive energy spectroscopy (EDS) and X-ray diffraction (XRD) analysis showed no trace of impurities and the possible formation of hydrated strontium oxide (H2O2Sr), respectively. The confocal laser microscopy (CLSM) analysis indicated that titanium samples treated with strontium (Sr) showed greater surface roughness. The acid/alkali treatment prior to the hydrothermal Sr deposition improved the surface free energy and resistance to corrosion of the Ti-15Mo alloy. The acid/alkali treatment also provided greater retention of the Sr particles on the Ti-15Mo surfaces accordingly with inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The AlamarBlue and fluorescence analysis indicated noncytotoxic effects against the MC3T3-E1 cells, which allowed cells adhesion and proliferation, with greater cells' spreading in the Sr-loaded Ti-15Mo samples. These findings suggest that Sr deposition by the hydrothermal method has the potential to enhance the physicochemical properties of the Ti-15Mo previously etched with H3PO4 and NaOH, and also improve the initial events related to cell-mediated bone deposition.

O tratamento de recessões gengivais além de proporcionar maior conforto ao paciente diminuindo a sensibilidade restabelece o posicionamento normal do tecido gengival melhorando a estética. Procedimentos cirúrgicos rápidos e com menor sintomatologia dolorosa seriam ideais na prática clínica para o recobrimento radicular. Dessa forma, biomateriais substitutos a enxertos autógenos estão cada vez mais sendo pesquisados. Assim, o objetivo deste estudo é verificar sob revisão da literatura científica atualizada, artigos clínicos que realizaram o recobrimento radicular em recessões dentais unitárias utilizando do Mucograft® comparativamente aos enxertos autógenos. Para isso, o termo Mucograft® foi utilizado para buscas no Pubmed e Google e 48 resultados foram obtidos. Desses, 9 artigos foram selecionados. 6 estudos avaliaram a porcentagem de recobrimento radicular, enquanto que 2 avaliaram o aumento da faixa de tecido queratinizado e 1 as mudanças histológicas. O uso de Mucograft® demonstrou resultados positivos em relação a capacidade de recobrimento radicular com boa estética e nível de dor pós operatória semelhante ao do obtido pelo enxerto autógeno. As evidências científicas demonstram que o a Matriz Mucograft® é um material promissor, mas comprovações com maior tempo de acompanhamento são necessárias para auxiliar o clínico nas suas escolhas.

O tratamento de recessões gengivais além de proporcionar maior conforto ao paciente diminuindo a sensibilidade restabelece o posicionamento normal do tecido gengival melhorando a estética. Procedimentos cirúrgicos rápidos e com menor sintomatologia dolorosa seriam ideais na prática clínica para o recobrimento radicular. Dessa forma, biomateriais substitutos a enxertos autógenos estão cada vez mais sendo pesquisados. Assim, o objetivo deste estudo é verificar sob revisão da literatura científica atualizada, artigos clínicos que realizaram o recobrimento radicular em recessões dentais unitárias utilizando do Mucograft® comparativamente aos enxertos autógenos. Para isso, o termo Mucograft® foi utilizado para buscas no Pubmed e Google e 48 resultados foram obtidos. Desses, 9 artigos foram selecionados. 6 estudos avaliaram a porcentagem de recobrimento radicular, enquanto que 2 avaliaram o aumento da faixa de tecido queratinizado e 1 as mudanças histológicas. O uso de Mucograft® demonstrou resultados positivos em relação a capacidade de recobrimento radicular com boa estética e nível de dor pós operatória semelhante ao do obtido pelo enxerto autógeno. As evidências científicas demonstram que o a Matriz Mucograft® é um material promissor, mas comprovações com maior tempo de acompanhamento são necessárias para auxiliar o clínico nas suas escolhas.

Modifications of implants by means of surface treatments are performed to optimize the biochemical interactions of the bone deposition process. However, if on the one hand they favor the adhesion of blood elements, on the other hand they can also enable the formation of biofilm. This study evaluated the adherence of blood cells and Escherichia coli in titanium discs submitted to surface treatments by anodic oxidation (OA), sandblasting followed by acid etching (JAT) compared with untreated discs (Li). To evaluate the adherence of microorganisms were performed: atomic force microscopy; Field Emission Gun Scanning Electron Microscopy (FEG-SEM); biofilm formation was evaluated by spectrophotometer turbidity analysis and colony forming unit (CFU/mL) before and after simulated brushing. For blood cell adherence, the blood collected from a patient was deposited and fixed on the discs and analyzed in the FEG-SEM being classified according to the "Blood Element Adherence Index". The results showed a slight increase in the adhesion of microorganisms in samples treated by anodic oxidation. However, the microorganisms were distributed singly and not in conglomerates, with no biofilm formation unlike the Li group. Regarding the adherence of blood elements, the Li group showed higher adherence and lower amount was found in the JAT group, but there was no statistically significant difference between the groups. The results of this study suggest that anodic oxidation treatment may favor the adherence of blood cells and fibrin mesh, contributing to the early stages of bone deposition.

This systematic review aims to identify and discuss the most used methodologies in pre-clinical studies for the evaluation of the implementation of dental implants in systemically compromised pigs and sheep. This study provides support and guidance for future research, as well as for the prevention of unnecessary animal wastage and sacrifice. Preferred Reporting for Systematic Reviews and Meta-Analyses (PRISMA) was used as a guideline; electronic searches were performed in PubMed, Scopus, Scielo, Web of Science, Embase, Science Direct, Brazilian Bibliography of Dentistry, Latin American and Caribbean Literature in Health Sciences, Directory of Open Access Journals, Database of Abstracts of Reviews of Effects, and gray literature until January 2022 (PROSPERO/CRD42021270119). Sixty-eight articles were chosen from the 2439 results. Most studies were conducted in pigs, mainly the Göttinger and Domesticus breeds. Healthy animals with implants installed in the jaws were predominant among the pig studies. Of the studies evaluating the effect of systemic diseases on osseointegration, 42% were performed in osteoporotic sheep, 32% in diabetic sheep, and 26% in diabetic pigs. Osteoporosis was primarily induced by bilateral ovariectomy and mainly assessed by X-ray densitometry. Diabetes was induced predominantly by intravenous streptozotocin and was confirmed by blood glucose analysis. Histological and histomorphometric analyses were the most frequently employed in the evaluation of osseointegration. The animal models presented unique methodologies for each species in the studies that evaluated dental implants in the context of systemic diseases. Understanding the most commonly used techniques will help methodological choices and the performance of future studies in implantology.

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