DETERMINAÇÃO DO CICLO REPRODUTIVO DA CUTIA (Dasypwcta prymnolopha) ATRAVÉS DO DIAGNÓSTICO COLPOCITOLÓGICO

Guimarães, Diva Anélie de Araújo; Moreira, D. de P.; Vale, W. G.

doi:10.1590/1809-43921997271064

Cited by 19 publications

(21 citation statements)

References 6 publications

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“…In other hand, progesterone combined with estradiol showed negative results for epithelial proliferation in vagina of Cavia porcellus. In G. spixii, the predominant cell types in the different phases of the estrous cycle were easier to detect during the proestrus and estrus by vaginal exfoliative cytology, similar to that found in other rodents such as Cavia porcellus (Selle 1922, Lilley et al 1997) Dasyprocta prymnolopha (Guimarães et al 1997), Agouti paca (Guimarães et al 2008) and Myocastor coypus (Felipe et al 2001). Our results also agree with those described in domestic mammals (Allison et al 2008).…”

Section: Discussionsupporting

confidence: 66%

“…(Guimarães et al 1997) 19-40 of rodents prymnolopha Agouti paca (Guimarães et al 2008) 24-42 Octodon degus (Mahoney et al 2011) 17-22 Myocastor coypus (Felipe et al 2001) 20-60 Thryonomys (Addo et al 2007) 27.8 ± 22.1 swinderianus Rattus norvegicus (Marcondes et al 2002) 4-5 Mus musculus (Mendonça et al 2007) 4…”

Section: Discussionmentioning

confidence: 99%

“…For a better monitoring of the estrous cycle phases, cells were counted in each sample of vaginal exfoliative cytology into fields containing from 0 to 100 cells in the cytological slides (Guimarães et al 1997). During the counting, cell types were grouped according to the criteria of morphology of each cell as above described.…”

Section: Animalsmentioning

confidence: 99%

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Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

et al. 2015

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RESUMO.-[Caracterização do ciclo estral de Galea spixii (Wagler, 1831).]Os Galea spixii habitam a vegetação semiá-rida da Caatinga, no Nordeste brasileiro. Eles são criados em cativeiro para realização de pesquisas relacionadas a biologia da reprodução. Sendo assim, o objetivo deste trabalho foi caracterizar o ciclo estral de G. spixii para obtenção de informações que melhorem o conhecimento do manejo reprodutivo da espécie em cativeiro. O ciclo estral foi monitorado por citologia esfoliativa vaginal em doze fêmeas adultas. Após a detecção de dois ciclos completos em cada animal, os mesmos foram eutanasiados. Em seguida foi realizado estudo histológico do epitélio vaginal com três fêmeas em cada fase do ciclo estral; cinco foram pareadas com machos para realização do grupo controle e outras três fêmeas foram utilizadas para monitorar a formação e ruptura da membrana de oclusão vaginal. Através de citologia esfoliativa vaginal, constatou-se predomínio de células superficiais em estro, células intermediárias grandes em proestro, células intermediárias pequenas e células parabasais com presença de neutrófilos em diestro e metaestro, respectivamente. O es- The Galea spixii inhabits semiarid vegetation of Caatinga in the Brazilian Northeast. They are bred in captivity for the development of researches on the biology of reproduction. Therefore, the aim of this study is characterize the estrous cycle of G. spixii, in order to provide information to a better knowledge of captive breeding of the species. The estrous cycle was monitored by vaginal exfoliative cytology in 12 adult females. After the detection of two complete cycles in each animal, the same were euthanized. Then, histological study of the vaginal epithelium, with three females in each phase of the estrous cycle was performed; five were paired with males for performing the control group for estrous cycle phases, and three other were used to monitor the formation and rupture of vaginal closure membrane. By vaginal exfoliative cytology, predominance of superficial cells in estrus, large intermediate cells in proestrus, intermediate and parabasal cells, with neutrophils, in diestrus and metestrus respectively was found. Estrus was detected by the presence of spermatozoa in the control group. By histology, greater proliferation of the vaginal epithelium in proestrus was observed. We conclude that the estrous cycle of G. spixii lasts 15.8 ± 1.4 days and that the vaginal closure membrane develops until complete occlusion of the vaginal ostium, breaking after few days. Future studies may reveal the importance of this fact for the reproductive success of this animal. INDEX TERMS: Galea spixii, cavies, female, preservation, reproduction, rodents. Characterization of the estrous cycle in

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

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Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

et al. 2015

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“…Os esfregaços foram analisados ao microscópio de luz (x400), sendo contadas 200 células em cinco diferentes campos. As células foram classificadas como: basais, parabasais, intermediárias e superficiais (Guimarães et al 1997). Para a delimitação do ciclo estral, foi considerado o intervalo compreendido entre dois metaestros, segundo os achados descritos por Guimarães et al (1997).…”

Section: Methodsunclassified

“…Posteriormente, Guimarães et al (1997) realizaram análises colpocitológicas para descrever as características do ciclo estral em Dasyprocta prymnolopha. De fato, sabe-se que as mudanças citológicas vaginais refletem os eventos endócrinos associados aos ciclos reprodutivos em algumas espécies mamíferas, podendo esta técnica ser utilizada com eficiência para predizer o seu período fértil (Moxon et al 2010).…”

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Monitoramento do ciclo estral de cutias (Dasyprocta leporina Lichtenstein, 1823) através de citologia esfoliativa vaginal e ultrassonografia

et al. 2015

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RESUMO.-O objetivo deste estudo foi monitorar o ciclo estral em cutias (Dasyprocta leporina) criadas em cativeiro no semiárido brasileiro. Durante 70 dias, cinco cutias foram diariamente submetidas a citologia esfoliativa vaginal, e o monitoramento ultrassonográfico ovariano foi realizado a cada três dias. Um total de 8 ciclos estrais foi completamente monitorado, com duração de 28,2±0,7 dias, variando de 24 a 31 dias. Pela citologia esfoliativa vaginal, houve uma predominância de células superficiais nas fases de proestro e estro (P<0,05), seguida da predominância de células intermediárias no metaestro (P<0,05) e de células parabasais no diestro (P<0,05). Por ultrassonografia, não houve diferenças na morfologia ovariana durante as diferentes fases do ciclo estral (P>0,05). Os folículos foram identificados durante as fases estrogênicas (proestro e estro), com diâ-metro médio de 1±0,5mm. Em apenas 12,5% das fases luteais, corpos lúteos medindo 1,4±0,9mm foram identificados. Conclui-se que a associação da citologia vaginal e da ultrassonografia ovariana constitui uma alternativa viável para o monitoramento de ciclos estrais e identificação das fases estrogênicas em cutias da espécie Dasyprocta leporina. TERMOS DE INDEXAÇÃO:Dasyprocta leporina, roedores, colpocitologia, ecografia ovariana. INTRODUÇÃOAs cutias (Dasyprocta sp.) são roedores utilizadas frequentemente como fonte de alimento e extração de sua pele, couro e pelos por populações humanas (Lopes et al. 2004 The objective of the study was to monitor the estrous cycle in agoutis (Dasyprocta leporina) bred in captivity in Brazilian semiarid. During 70 days, five agoutis were daily subjected to vaginal exfoliative cytology, and the ovarian ultrasound monitoring was conducted every three days. A total of 8 estrous cycles were completely monitored, lasting 28.2±0.7 days, ranging from 24 to 31 days. By vaginal exfoliative cytology, there was predominance of superficial cells at proestrus and estrus phases (P<0.05), followed by the predominance of intermediate cells in the metestrus (P<0.05) and parabasal cells in diestrus (P<0.05). By ultrasound, there were no differences in ovarian morphology during the different phases of the estrous cycle (P>0.05). Follicles during the estrogenic phases (proestrus and estrus) were identified, with an average diameter of 1±0.5mm. In only 12.5% of luteal phases, corpora lutea measuring 1.4±0.9mm were identified. We conclude that the association of vaginal cytology and ovarian ultrasonography is a useful alternative for monitoring the estrous cycle and identifying the estrogenic phases in Dasyprocta leporina.INDEX TERMS: Dasyprocta leporina, rodents, colpocytology, ovarian echography.

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Follicular development and morphological changes in the vaginal epithelium during the estrous cycle ofGalea spixii

Santos¹,

Viana²,

Oliveira

et al. 2016

Microsc. Res. Tech.

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The current study aimed to determine if characteristics observed in vaginal cytology during the estrous cycle of female SYT cavies corresponded with proliferation of the vaginal epithelium, characterized by proliferating cell nuclear antigen (PCNA) immunolocalization, and with follicular development at different phases of the estrous cycle. After determining estrous cycle phases by vaginal cytology, females were euthanized at metestrus, diestrus, proestrus, and estrus. Histological study of the vaginal epithelium and ovary were then performed. Immunohistochemistry for PCNA in vaginal tissue at each cycle phase was also performed. Superficial cornified cells and early post-ovulatory follicles were found at estrus. Few nuclei below the enucleate superficial cells were immunoreactive to PCNA. At metestrus, the vaginal epithelium underwent desquamation and lost the superficial cornified cells; basal and intermediate cells appeared, and the post-ovulatory follicle formed an early corpus luteum. No PCNA immunoreactivity was observed. At diestrus, the corpus luteum was developed, and the vaginal epithelium contained basal and intermediate cells. There was PCNA immunoreactivity in the cellular nucleus in the germinative stratum of the epithelium. Because of the growth and maturation of ovarian follicles, the vaginal epithelium suffered intense proliferation at proestrus. Vaginal cytology revealed large intermediate cells and nucleated and enucleated superficial cornified cells. In the ovary, mature follicles were present. More apparent immunoreactivity of PCNA in the germinative layer was found. In summary, we inferred that vaginal exfoliative findings matched the proliferation process of the vaginal epithelium. PCNA immunolocalization occurred as well as corresponding follicular development in the ovaries.

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DETERMINAÇÃO DO CICLO REPRODUTIVO DA CUTIA (Dasypwcta prymnolopha) ATRAVÉS DO DIAGNÓSTICO COLPOCITOLÓGICO

Cited by 19 publications

References 6 publications

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

Characterization of the estrous cycle in Galea spixii(Wagler, 1831)

Monitoramento do ciclo estral de cutias (Dasyprocta leporina Lichtenstein, 1823) através de citologia esfoliativa vaginal e ultrassonografia

Follicular development and morphological changes in the vaginal epithelium during the estrous cycle ofGalea spixii

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