Métodos de determinar a idade fisiológica em Diptera de importância médica. Uma revisão com especial referência aos vetores de doenças na América do Sul.

Charlwood, J. D.; Rafael, J. A.; Wilkes, T. J.

doi:10.1590/1809-43921980102311

Cited by 16 publications

(5 citation statements)

References 8 publications

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“…The physiological age (Parity) of the mosquitoes is also related to their biting activities. Studies of natural populations of Kerteszia , based in the Polovodova method [ 34 ] to determine the physiological age, indicated that An. cruzii and An.…”

Section: Introductionmentioning

confidence: 99%

Kerteszia subgenus of Anopheles associated with the Brazilian Atlantic rainforest:current knowledge and future challenges

Marrelli

Malafronte

Sallum

et al. 2007

Malar J

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Background: The Atlantic rainforest ecosystem, where bromeliads are abundant, provides an excellent environment for Kerteszia species, because these anophelines use the axils of those plants as larval habitat. Anopheles (K.) cruzii and Anopheles (K.) bellator are considered the primary vectors of malaria in the Atlantic forest. Although the incidence of malaria has declined in some areas of the Atlantic forest, autochthonous cases are still registered every year, with Anopheles cruzii being considered to be a primary vector of both human and simian Plasmodium.

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Section: Introductionmentioning

confidence: 99%

Kerteszia subgenus of Anopheles associated with the Brazilian Atlantic rainforest:current knowledge and future challenges

Marrelli

Malafronte

Sallum

et al. 2007

Malar J

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“…Reviews of techniques for age determination in insects of medical importance, including the methods used in the present study, have been presented by Charlwood, Rafael & Wilkes (1980) and Tyndale-Biscoe (1984). Ovaries of Cx.…”

Section: Discussionmentioning

confidence: 99%

‘We like it wet’: a comparison between dissection techniques for the assessment of parity inAnopheles arabiensisand determination of sac stage in mosquitoes alive or dead on collection

Charlwood

Tomás²,

Andegiorgish

et al. 2018

PeerJ

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BackgroundThe determination of parous rates in mosquitoes, despite numerous shortcomings, remains a tool to evaluate the effectiveness of control programs and to determine vectorial capacity in malaria vectors. Two dissection techniques are used for this. For one, the tracheoles of dried ovaries are examined with a compound microscope and in the other the follicular stalk of ovaries is examined, wet, with a stereomicroscope. The second method also enables the sac stage of parous insects (which provides information on the duration of the oviposition cycle) and the mated status of insects to be determined. Despite widespread use the two techniques have not previously been compared.MethodsWe compared the two dissection techniques using Anopheles arabiensis, collected with a tent-trap in Eritrea. The paired ovaries were removed in water and one was examined by each method. From a separate set of dissections from Tanzania, we also determined if the sac stages of Anopheles gambiae s.l. (83% of 183 identified by PCR being Anopheles arabiensis the remainder being A. gambiae) that were alive on collection were different to those that died on collection and what the implications for vectorial capacity estimation might be.ResultsSeven per cent of the dry ovaries could not be classified due to granulation (yolk) in the ovariole that obscured the tracheoles. The sensitivity of the dry dissection was 88.51% (CI [79.88–94.35%]) and the specificity was 93.55% (CI [87.68–97.17%]) among the 211 ovaries that could be classified by the dry technique and compared to the ovaries dissected wet. 1,823 live and 1,416 dead from Furvela tent-traps, CDC light-trap and window-trap collections were dissected ‘wet’ from Tanzania. In these collections parous insects were more likely to die compared to nulliparous ones. The proportion of parous mosquitoes with ‘a’ sacs (indicative of recent oviposition) was significantly greater in insects that were dead (0.36) on collection in the morning compared to those that were alive (0.12) (Chi square 138.93, p < 0.001). There was a preponderance of newly emerged virgin insects in the outdoor collection (Chi sq = 8.84, p = 0.003).ConclusionsIn anophelines the examination of mosquito ovaries using transmitted light in a ‘wet’ dissection is a more useful and informative technique than examination of dry ovaries. In order to correctly estimate the duration of the oviposition cycle mosquitoes should be dissected as soon as possible after collection. Younger insects were more likely to attempt to feed outdoors rather than indoors.

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“…Sin embargo, este comportamiento no se repitió en los municipios de Piedecuesta y Giron, en estos lugares, como se detectó para hembras de A. aegypti, las cuales evidenciaron en su paridad el paso por uno y dos ciclos gonadotróficos lo cuál concuerda con los reportes para esta especie en Sao Paulo y Rio de Janeiro en Brasil 12,36 . De cualquier forma, estos resultados aunque no son nuevos para estas especies, si evidencian una aproximación de lo que puede estar pasando en el área metropolitana de Bucaramanga sobre la discordancia gonadotrofica de estos zancudos, lo cuál en su comportamiento indica que realiza varias alimentaciones hasta completar la cantidad de sangre adecuada para madurar sus huevos y ovipositar 26,[37][38][39][40] .…”

Section: Discussionunclassified

“…En el octavo mes de muestreo correspondiente a mayo y junio se realizó una colecta utilizando trampas para hembras grávidas StegTrap 24 cebadas con agua instaladas por cinco días para capturar vivos los especímenes para las pruebas, además de este método se uso un aspirador eléctrico accionado durante 15 min para captura de los adultos presentes en las viviendas (peridomicilio-intradomicilio), paralelo al muestreo de las trampas (ovitrampas), a este material se le determinó el número de ciclos gonadotróficos [25][26][27][28][29][30] .…”

Section: Estado Gonadotróficounclassified

Dinámica de oviposición de Aedes (Stegomyia) aegypti (Diptera: Culicidae), estado gonadotróficoy coexistencia con otros culícidos en el área Metropolitana de Bucaramanga, Colombia

Ruiz

Rincón

Parra

et al. 2018

revsal

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Introduction: Tropical urban areas favor the presence of Aedes aegypti in the American continent. Other mosquitoes can share the same geographic distribution with this mosquito, which justifies that they must be monitored. Objective: It is to know the dynamics of oviposition of A. aegypti, evaluate its gonadotrophic state and determine taxonomically the presence of other culicidae in the metropolitan area of Bucaramanga. Methodology: Oviposition traps (N = 960) were baited with hay infusion (10%) for one year in four locations (Norte, Girón, Piedecuesta and Floridablanca). The ovitraps were kept for five days indoors and outdoors, while larvae were collected in plants such as bromeliads and water reservoirs from each collecting site. In the month of May 2014, were captured with an electric aspirator and traps to catch females of A. aegypti and Culex quinquefasciatus to which were estimated their gonadotrophic state. Environmental data such as precipitation and temperature were correlated with the collected eggs and their oviposition indices. Results: 25,254 eggs of A. aegypti were obtained (39.1% Girón, 36.8% Bucaramanga, 15.2% Floridablanca and 8.9% Piedecuesta). In general, oviposition rates were higher outdoors. As for the gonotrophic state, it was detected that females of A. aegypti can have for over two gonadotrophic cycles. In the active catch outdoors were found Culex spp., Culex quinquefasciatus, Limatus durhamii, Toxorhynchites spp and Wyeomyia davisi. Conclusion: A. aegypti was present in all localities and other mosquitoes are reported in the metropolitan area.

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Métodos de determinar a idade fisiológica em Diptera de importância médica. Uma revisão com especial referência aos vetores de doenças na América do Sul.

Cited by 16 publications

References 8 publications

Kerteszia subgenus of Anopheles associated with the Brazilian Atlantic rainforest:current knowledge and future challenges

Kerteszia subgenus of Anopheles associated with the Brazilian Atlantic rainforest:current knowledge and future challenges

‘We like it wet’: a comparison between dissection techniques for the assessment of parity inAnopheles arabiensisand determination of sac stage in mosquitoes alive or dead on collection

Dinámica de oviposición de Aedes (Stegomyia) aegypti (Diptera: Culicidae), estado gonadotróficoy coexistencia con otros culícidos en el área Metropolitana de Bucaramanga, Colombia

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