The literature highlights that a severe infection by the fungus Colletotrichum truncatum may be capable of inflicting considerable damage to seeds after harvest, potentially affecting their chemical composition and physiological quality. Taking into account that currently there is no categorization in terms of susceptibility and tolerance on this pathogen, the present work is presented with the main objective of "Evaluate the influence of Pathogenicity of C. truncatum on the physiological quality (germination, vigor, viability) and biochemical components in different varieties of soybean seeds (Glycine max)" most planted in the region. The work was carried out in the Agrotec laboratory, located in the Municipality of San Alberto (Alto Paraná), using a completely randomized experimental design, with AxB factorial arrangement, where A indicates ten most planted soybean varieties in the region and B with or without artificial inoculation of Colletotrichum truncatum, with twenty treatments and four repetitions. The variables evaluated were: germination, vigor, viability and chemical composition. The data were subjected to analysis of variance and the Tukey test at 5% error. The results showed a significant statistical difference, accepting the alternative hypothesis proposed "The pathogenicity of Colletotrichum truncatum influences the physiological quality (germination, vigor, viability) and biochemical components (saturated and unsaturated fatty acids) in different varieties of soybean seeds (Glycine max)".