Genetic variability of Corynespora cassiicola isolates from Amazonas, Brazil

Abstract

Corynespora cassiicola is a cosmopolitan ascomycete widely known as phytopathogen in several crops, and more recently as an emerging pathogen in humans. In this study the genetic variability of 60 isolates of Corynespora cassiicola from different hosts and cities of Amazonas was evaluated, using AFLP molecular markers. Seven genetic groups were identified according to a dendrogram obtained by the Unweighted Pair Group Method using Arithmetical Averages, indicating significant variability among the isolates. Three isolates of different hosts (28, obtained from papaya; 55, obtained from cucumber; and 58, from tomato) remained as single individuals in distinct groups, suggesting marked genetic variation in comparison to the other isolates and possible specificity by the host.

The fungal pathogen Corynespora cassiicola: A review and insights for target spot management on cotton and Soya bean

“…The wide host range of C. cassiicola and its cultivar preference were explained based on the pathogen diversity (Déon et al, 2014). Different genes of C. cassiicola have been amplified to construct phylogenetic trees to understand the evolution and variability of C. cassiicola isolates and to help build disease management strategies (Banguela-Castillo et al, 2020;Bentes et al, 2018;Déon et al, 2014;Dixon et al, 2009;Hieu et al, 2020;Nghia et al, 2008;Oktavia et al, 2017;Qi et al, ,2009Qi et al, , , 2011Shimomoto et al, 2011;Shrestha et al, 2017;Silva et al, 2003;Wu et al, 2019). Dixon et al, (2009) conducted the most prominent study on (Qi et al, 2009).…”

Morphological and genetic characterization of Corynespora cassiicola isolates obtained from roselle and associated weeds

“…The genetic characterization of C. cassiicola has been evaluated using different techniques, including RAPD, ISSR, AFLP, iPBS and phylogeny (Silva et al, 2003;Dixon et al, 2009;Qi et al, 2011;Deon et al, 2014;Oktavia et al, 2017;Silva et al, 2018;Wu et al, 2019). In the case of phylogeny, partial regions of the β-tubulin gene, 1-α elongation factor (EF-1α), calmodulin and actin have been amplified, as well as internal transcribed spacers (Shimomoto et al, 2011;Oktavia et al, 2017).…”

“…La caracterización genética de C. cassiicola se ha estudiado con técnicas como RAPDs, ISSR, AFLP, iPBS y filogenia (Silva et al, 2003;Dixon et al, 2009;Qi et al, 2011;Deon et al, 2014;Oktavia et al, 2017;Silva et al, 2018;Wu et al, 2019). En este último caso, se han amplificado regiones parciales de los genes β-tubulina, factor de elongación 1-α (EF-1α), calmodulina y actina, además de los espaciadores transcritos internos (Shimomoto et al, 2011;Oktavia et al, 2017).…”

“…Las colonias obtenidas se purificaron mediante la técnica de cultivo monospórico. and epidemiological studies and for developing management strategies (Qi et al, 2011;Oktavia et al, 2017;Sumabat et al, 2018;Silva et al, 2018). Since there are no studies about the characterization of this fungus in Mexico, the objective of this study was to characterize morphological and genetically several C. cassiicola isolates obtained from roselle leaves and calyxes, as well as weeds associated with the crop in the state of Guerrero.…”

“…y determinaron que aquellos geográficamente distintos pero obtenidos de la misma especie vegetal eran muy similares genéticamente, lo cual sugería cierto grado de especialización con el hospedante (Sumabat et al, 2018). La especificidad de aislados de C. cassiicola con su hospedante también ha sido sugerida en el caso de papayo, tomate y pepino (Silva et al, 2018). Sin embargo, hasta la fecha no es posible establecer de manera definitiva una asociación de los grupos filogenéticos de C. cassiicola con su origen geográfico u hospedantes (Deon et al, 2014).…”