Morphological and genetic characterization of Corynespora cassiicola isolates obtained from roselle and associated weeds

Ortega-Acosta, Santo Ángel; Ochoa-Martínez, Daniel Leobardo; Hérnández-Morales, Javier; Palemón-Alberto, Francisco

doi:10.18781/r.mex.fit.1909-2

Cited by 3 publications

(4 citation statements)

References 18 publications

(74 reference statements)

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“…For the inoculation of roselle genotypes, the strain CC47GRO of Corynespora cassiicola was used (GenBank: MF000875) (16). To obtain sufficient inoculum, the fungus was increased in Petri dishes containing Potato Dextrose Agar (PDA) culture medium (BIOXON ® ) and subsequently placed in an incubator (NOVATECH ® ) for 14 days at 28°C (14).…”

Section: Inoculum Sourcementioning

confidence: 99%

Selection of roselle genotypes tolerant/resistant to spots induced by Corynespora cassiicola in Guerrero, Mexico

et al. 2022

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Roselle spot caused by Corynespora cassiicola affects calyx production in Mexico. The aim of the present study was to evaluate twenty-two roselle genotypes against C. cassiicola under greenhouse conditions. The area under the disease progress curve (AUDPC) was used to detect susceptible, tolerant and resistant genotypes. Considering the incidence in leaves, susceptible genotypes were Conpoz, Sudlaz, Morcot and Sudigua (AUDPC values of 7379.2 to 5891.7); tolerant genotypes were Conayu and Chinxal (AUDPC 1341.6 and 1313.2). Based on the severity in leaves, susceptible genotypes were Sudigua, Conpoz and Morena2 (AUDPC from 788.4 to 436.3); tolerant genotypes were Almatech, Crioxal, Rojatec and Conayu (37.7 to 28.76 AUDPC). Assessment of the incidence in calyces indicated that susceptible genotypes were Morena2, Morena, Conpoz, Descop and Chinayu (AUDPC from 6572.1 to 2319.4); for the severity in calices, susceptible genotypes were Morena2, Descop, Morena, Cabxal, Chinayu and Conpoz (AUDPC from 3355.4 to 779.2). Incidence and severity on calyces evidenced resistant genotypes, which were Almatech, Criolaz2, Criocot, Criolaz, Rojatec, Criopoz, Crioxal, Chinxal and Conayu (0.0 AUDPC). In the present study, tolerant and resistant genotypes were detected to leaf and calyx spot caused by C. cassiicola in roselle.

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Section: Inoculum Sourcementioning

confidence: 99%

Selection of roselle genotypes tolerant/resistant to spots induced by Corynespora cassiicola in Guerrero, Mexico

et al. 2022

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“…The CCPER4 strain produced dense, compact and black-greyish mycelium, and straight-to-curve conidia with short light-brown pseudosepta that measured 57.0-308.2 x 10-20 µm (length x width, respectively), which are distinctive characteristics of C. cassiicola (Qi et al, 2011;Ortega-Acosta et al, 2020b).…”

Section: Fully Bilingualmentioning

confidence: 99%

“…Revista Mexicana de FITOPATOLOGÍA Mexican Journal of Phytopathology respecto al control; estas cepas fueron seleccionadas para realizar los ensayos de control en condiciones de bioespacio (Cuadro 1). La cepa CCPER4 mostró micelio denso y compacto, de color negro grisáceo, con conidios rectos a curvos, con pseudoseptos, cortos, de coloración marrón claro, con dimensiones de 57.0-308.2 x 10-20 µm (largo x ancho, respectivamente), características distintivas de C. cassiicola (Qi et al, 2011;Ortega-Acosta et al, 2020b).…”

Section: Fully Bilingualunclassified

Antagonistic bacteria for biospace control of roselle spot (Corynespora cassiicola) of Hibiscus sabdariffa

Patricio-Hernández¹,

Ortega-Acosta²,

Ramírez-Peralta³

et al. 2020

RMF

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En años recientes, el manchado de la jamaica inducido por Corynespora cassiicola amenaza la producción de cálices. El objetivo fue determinar si algunas cepas bacterianas inhiben el desarrollo de C. cassiicola in vitro y bajo condiciones de bioespacio. La inhibición del desarrollo fue por confrontación dual de las bacterias: alimentos (20), piel de anfibios (41), jales mineros (22), aire (siete) y rizosfera (10) contra el hongo. Se sembraron plantas de jamaica variedad “criolla” y se inocularon artificialmente con C. cassiicola, a partir del inicio de síntomas de la enfermedad se inocularon las cepas bacterianas seleccionadas con mayor porcentaje de inhibición. Se evaluó la ABCPE. Solo siete cepas inhibieron del 62.7 al 100% el desarrollo del hongo in vitro: Klebsiella pneumoniae (M10-1 y M10-10), Acinetobacter lwoffii (A5), Sphingomonas paucimobilis (NF21), Serratia marcescens (M13ACD), S. liquefaciens (M8ACD) y Acinetobacter sp. (5H2). En relación a la severidad en hojas las cepas M10-1 y M10-10 redujeron el ABCPE en un 9.5 y 12.6%, respectivamente, seguido de NF21 (14.5 de ABCPE), el control registró los mayores niveles (45.7 de ABCPE). Para la severidad en cálices, los análisis del ABCPE de todas las cepas ejercieron control al reducir niveles de severidad (17.1-8.6 ABCPE).

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“…Although seed transmission of the beet curly top and abutilon mosaic viruses had been theorized since 1868 [ 10 ], conclusive proof for seed transmission of begomoviruses utilizing virus-specific molecular methods was only presented in 2015 for the sweet potato leaf curl virus (SPLCV) [ 11 ]. Transmission from seed to seedling was subsequently demonstrated in about 13 host–virus combinations—tomato yellow leaf curl virus (TYLCV) in soybean and sweet pepper [ 12 , 13 ], mungbean yellow mosaic virus (MYMV) in blackgram [ 14 ], tomato leaf curl New Delhi virus (ToLCNDV) in chayote [ 15 ] and zucchini squash [ 16 ], BgYMV in bitter gourd [ 9 ], dolichos yellow mosaic virus (DoYMV) in lablab bean [ 17 ], sweet potato symptomless virus I (SPSMV) in sweet potato [ 18 ] and pepper leaf curl Indonesia virus in chili capsicum (PepYLCIV) [ 19 ], and okra yellow mosaic Mexico virus (OYMMV) [ 20 ]. Manivannan et al [ 9 ] recorded seed infectivity by BgYMV up to 79.16% and a transmission rate of 32% in seedlings.…”

Section: Introductionmentioning

confidence: 99%

Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

Devi

Jothika

Sankari

et al. 2023

Plants

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Bitter gourd (Momordica charantia L.), one of the valued vegetable crops in India, is severely affected by yellow mosaic disease caused by two begomoviruses, tomato leaf curl New Delhi virus (ToLCNDV) and bitter gourd yellow mosaic virus (BgYMV). The symptoms are yellowing, distortion of leaf, puckering, and malformed fruits. Increased incidence of the disease and appearance of symptoms even in young emerging seedling stage were suggestive of seed transmission of the viruses, which was examined in detail. To study the seed transmission, two sources—seeds of elite hybrids H1, H2, H3, H4, and Co1 procured from a seed market; and seeds from infected plants in the farmer’s field were tested. Detection of the virus by DAS-ELISA using polyclonal antibody indicated embryo infection up to 63%, 26%, 20%, and 10% in hybrids H1, H2, H3, and H4, respectively, for market-procured seeds. In PCR analysis with primers specific for ToLCNDV and BgYMV, infection by ToLCNDV was as high as 76% and mixed infection was 24%. In contrast, in seeds derived from field-infected plants, the percentage detection was less. Grow-out tests with market-procured seeds revealed no transmission for BgYMV compared with 5% transmission for ToLCNDV. Whether seed-borne inocula could serve as an inoculum for new infection in a field and further progress of the disease was investigated in a microplot study. The study clearly revealed variation in seed transmission between different sources, lots, cultivars, and viruses. The virus present in symptomatic and asymptomatic plants was easily transmitted by whitefly. In another microplot experiment, the potential of seed-borne virus as inoculum was proved. There was 43.3% initial seed transmission in the microplot, increasing to 70% after release of 60 whiteflies.

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Morphological and genetic characterization of Corynespora cassiicola isolates obtained from roselle and associated weeds

Cited by 3 publications

References 18 publications

Selection of roselle genotypes tolerant/resistant to spots induced by Corynespora cassiicola in Guerrero, Mexico

Selection of roselle genotypes tolerant/resistant to spots induced by Corynespora cassiicola in Guerrero, Mexico

Antagonistic bacteria for biospace control of roselle spot (Corynespora cassiicola) of Hibiscus sabdariffa

Seed Transmission of Begomoviruses: A Potential Threat for Bitter Gourd Cultivation

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