Isolation, bioprospecting and diversity of salt-tolerant bacteria associated with sugarcane in soils of Pernambuco, Brazil

Leite, Maria C. B. S.; Farias, Andreza Raquel Barbosa de; Freire, Fernando José; Andreote, Fernando Dini; Kuklinsky-Sobral, Júlia; Freire, Maria Betânia Galvão dos Santos

doi:10.1590/1807-1929/agriambi.v18nsupps73-s79

Cited by 17 publications

(6 citation statements)

References 14 publications

(9 reference statements)

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“…Concerning the isolated bacteria, 30.88 % were from roots, 26.48 % from the rhizosphere, 32.35 % from leaves and 10.29 % from stem. These data corroborate those reported by Silva et al (2012) and Leite et al (2014), who isolated bacteria in nonselective media and also found a higher number of microorganisms from root, when compared to the rhizosphere.…”

Section: Resultssupporting

confidence: 92%

Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Rodrigues

Forzani

Soares

et al. 2016

Pesqui. Agropecu. Trop.

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Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers and increase the resistance to biotic and abiotic stress. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with sugarcane, as well as assessing their ability to promote plant growth. Endophytic bacteria from leaf, stem, root and rhizosphere were isolated from the RB 867515 commercial sugarcane variety and screened for indole acetic acid (IAA) production, ability to solubilize phosphate, fix nitrogen and produce hydrogen cyanide (HCN), ammonia and the enzymes pectinase, cellulase and chitinase. A total of 136 bacteria were isolated, with 83 of them presenting some plant growth mechanism: 47 % phosphate solubilizers, 26 % nitrogen fixers and 57 % producing IAA, 0.7 % HCN and chitinase, 45 % ammonia, 30 % cellulose and 8 % pectinase. The seven best isolates were tested for their ability to promote plant growth in maize. The isolates tested for plant growth promotion belong to the Enterobacteriaceae family and the Klebsiella, Enterobacter and Pantoea genera. Five isolates promoted plant growth in greenhouse experiments, showing potential as biofertilizers.

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Section: Resultssupporting

confidence: 92%

Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Rodrigues

Forzani

Soares

et al. 2016

Pesqui. Agropecu. Trop.

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“…However, A. diazotrophicus colonization of sugar cane is inhibited by high N‐fertilization (Fuentes‐Ramı́rez et al ., ), and exogenous nitrogen fertilizer has been demonstrated to reduce the number of diazotrophic endophytes cultured from sugar cane (Pariona‐Llanos et al ., ). In Brazil, both rhizosphere and endophyte populations were demonstrated to be altered following the practice of adding vinasse, a concentrated by‐product of the sugar extraction process rich in nutrients, back to soils to fertilize sugar cane (Leite et al ., ). These data indicate that diazotrophic relationships may not be retained by the plant in conditions where they are not conveying a benefit in terms of nitrogen availability.…”

Section: Plant Benefits and Near‐term Exploitationmentioning

confidence: 97%

“…In a study of 102 bacteria associated with sugar cane roots, 74 were able to fix nitrogen and 77 were able to solubilize phosphate, all 102 produced IAA to at least some degree, 50 were positive for the production of the QS molecule N ‐acyl homoserine lactone (AHL), and 33 isolates were positive for all four tests. Twenty‐seven isolates were further tested for salinity tolerance (Leite et al ., ). Harnessing these benefits to promote biomass crop growth will require a combination of detailed understanding of the component microbes and their interactions with plants, for example by mutagenesis (Rouws et al ., ), and also long‐term field studies to determine the factors regulating microbial populations in the rhizosphere and soil.…”

Section: Plant Benefits and Near‐term Exploitationmentioning

confidence: 97%

Understanding and engineering beneficial plant–microbe interactions: plant growth promotion in energy crops

Farrar

Bryant

Cope-Selby

2014

Plant Biotechnology Journal

240

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Plant production systems globally must be optimized to produce stable high yields from limited land under changing and variable climates. Demands for food, animal feed, and feedstocks for bioenergy and biorefining applications, are increasing with population growth, urbanization and affluence. Low-input, sustainable, alternatives to petrochemical-derived fertilizers and pesticides are required to reduce input costs and maintain or increase yields, with potential biological solutions having an important role to play. In contrast to crops that have been bred for food, many bioenergy crops are largely undomesticated, and so there is an opportunity to harness beneficial plant–microbe relationships which may have been inadvertently lost through intensive crop breeding. Plant–microbe interactions span a wide range of relationships in which one or both of the organisms may have a beneficial, neutral or negative effect on the other partner. A relatively small number of beneficial plant–microbe interactions are well understood and already exploited; however, others remain understudied and represent an untapped reservoir for optimizing plant production. There may be near-term applications for bacterial strains as microbial biopesticides and biofertilizers to increase biomass yield from energy crops grown on land unsuitable for food production. Longer term aims involve the design of synthetic genetic circuits within and between the host and microbes to optimize plant production. A highly exciting prospect is that endosymbionts comprise a unique resource of reduced complexity microbial genomes with adaptive traits of great interest for a wide variety of applications.

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“…The selection of isolates with more than one characteristic of plant growth promotion has been receiving more attention from the researchers, due to the possibility of multiple functions in crop management programs (SHIOMI et al, 2009;LEITE et al, 2014).…”

Section: Selection Of Exopolysaccharide-producing Bacteria (Eps)mentioning

confidence: 99%

Bioprospection and genetic diversity of endophytic bacteria associated with cassava plant

et al. 2018

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-Cassava is mostly planted in sandy soils which are usually of low fertility, thereby making it necessary to perform beneficial associations with microorganisms that can promote their growth. In this perspective, the possibility of selecting bacterial isolates efficient in promoting the growth of the culture is evident, which can provide subsidies for future inoculants. The objective of this study was to isolate, identify, select and evaluate the genetic diversity of endophytic bacteria in roots and stems of cassava grown in Garanhuns -PE, with features involved in promoting plant growth. The isolation was performed on culture medium semisolid LGI-P. The selected isolates were evaluated for the potential to fix N 2 , as the ability to produce indole acetic acid, for their ability to solubilize inorganic phosphate and produce exopolysaccharides. Some bacterial isolates had their 16S rRNA gene sequenced by the Sanger method. A total of 52 endophytic bacteria isolates were obtained from cassava. Regarding the potential to fix N 2 , 15% of the isolates were positive. As for the production of IAA, 78% of the isolates produced this phytohormone in a medium with increased L-tryptophan. Approximately 31% of the isolates were able to solubilize inorganic phosphate and 60% had exopolysaccharide. The identification of 19 isolates allowed the grouping into six bacterial genera, namely: Achromobacter, Bacillus, Burkholderia, Enterobacter, Pantoea and Pseudomonas. Cassava plants grown in Garanhuns -PE present interaction with different groups of endophytic bacteria and there are bacterial groups with several characteristics involved in promoting plant growth.Keywords: 16S rRNA. Exopolysaccharide. Indole acetic acid. Biological nitrogen fixation. P solubilization. BIOPROSPECÇÃO E DIVERSIDADE GENÉTICA DE BACTÉRIAS ENDOFÍTICAS ASSOCIADASA PLANTAS DE MANDIOCA RESUMO -A mandioca é, geralmente, plantada em solos arenosos e de baixa fertilidade, tornando eminente a necessidade da mesma em realizar associações benéficas com micro-organismos que possam auxiliar seu crescimento. Nessa ótica, é evidente a possibilidade de seleção de isolados bacterianos eficientes em promover o crescimento da cultura, os quais podem fornecer subsídios para futuros inoculantes. O objetivo deste trabalho foi isolar, identificar, selecionar e avaliar a diversidade genética de bactérias endofíticas de raízes e caules de mandioca, cultivada em Garanhuns -PE, com características envolvidas na promoção de crescimento vegetal. O isolamento foi realizado em meio de cultura semissólido LGI-P. Os isolados selecionados foram avaliados quanto ao potencial de fixar N 2 , quanto à capacidade de produzir ácido indol acético, quanto à capacidade de solubilizar fosfato inorgânico e produzir exopolissacarídeos.

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Isolation, bioprospecting and diversity of salt-tolerant bacteria associated with sugarcane in soils of Pernambuco, Brazil

Cited by 17 publications

References 14 publications

Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Understanding and engineering beneficial plant–microbe interactions: plant growth promotion in energy crops

Bioprospection and genetic diversity of endophytic bacteria associated with cassava plant

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