ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

Most of the medicinal, aromatic and other commercially important crops are poor rooters, and some of them are sessile in terms of seed production; hence these plants are very difficult to propagate either through stem cuttings based vegetative propagation or through seedlings based sexual propagation. During the last two decades, plant tissue culture has emerged as an alternative technique for the propagation of plants with commercial importance. Majorly, the somatic tissues, viz., leaf, node and shoot tip, are being used as explants for the production of genetically similar plantlets through tissue culture studies. Recently, abnormalities with respect to ploidy level and genetic fidelity have been reported in In Vitro regenerated plantlets. This is mainly due to the usage of synthetic chemicals or artificial plant growth regulators in In Vitro culture studies, the fragile nature of callus and exposure of cultures to artificial light sources. In order to ensure the commercial production of genetically true clones of commercial plants, nowadays it has become an obligatory step to assess the ploidy level and genetic fidelity of regenerated plantlets with that of mother plants. This book chapter focussed on different molecular techniques which are in use for the detection of ploidy level and genetic fidelity of In Vitro micro propagated plantlets.

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Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys

Faria

Ribeiro-Kumara

Costa

et al. 2022

NZJFS

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Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species.

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ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

Cited by 5 publications

References 52 publications

Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

Molecular Techniques for the Detection of Ploidy Level and Genetic Fidelity of Regenerated Plantlets

Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys

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