Inhibitory Effects of Some Flavonoids on Thioredoxin Reductase Purified from Chicken Liver

Abstract

Thioredoxin reductases (TrxRs) are selenocysteine-containing flavoenzymes that reduce Trxin NADPH-dependent manner. In the view of the direct vital role of TrxR in a wide range of biochemical and physiological processes, methods to inhibit this enzyme are clinically important. TrxR has recently emerged as a new candidate in anticancer drug investigations because of overexpression in tumorous cells. In this study, TrxR from chick liver was purified 94.6-fold with a yield of 4.86% and a specific activity of 0.19 EU/mg. K M and V max values of TrxR for DTNB were calculated as 0.9 mM and 0,03 EU/mL, respectively. Then, the effects of the flavonoids hesperidin, naringenin, chlorogenic acid, ferulic acid, naringin, 3,4-dihydoxybenzoic acid, and ellagic acid on the enzyme activity were evaluated under in-vitro conditions. Ellagic acid showed the strongest inhibitory activity on TrxR with a IC 50 value of 18 µM, followed by naringenin and chlorogenic acid with IC 50 values of 46.7 µM and 75.8 µM, respectively. Our results showed that flavonoids as natural products are potential inhibitors of TrxRs and the flavonoid content in animal diets may alter metabolic pathways by influencing TrxR activity.

In vitro Carbonic Anhydrase Inhibitory Effects of The Extracts of Satureja cuneifolia

“…However, natural products have been important candidates in pharmaceutical industry and acted as vital position for the discovery of novel drugs. These kinds of products may possess the ability for binding to specific protein-based biomolecules whose functions are crucial in the treatment of many diseases (Türkoğlu et al, 2019).…”