In vitro Carbonic Anhydrase Inhibitory Effects of The Extracts of Satureja cuneifolia

Abstract

Carbonic anhydrases catalyze the interconversion between carbon dioxide and bicarbonate. Carbonic anhydrase inhibition has therapeutic importance and there are many drugs which use this mechanism. Carbonic anhydrase inhibitors are used as diuretic, antiglaucoma, antitumor and antiepileptic agents. As an alternative for synthetic chemicals with strong side effects, natural products have gained popularity in the recent years. In this study, Satureja cuneifolia, a medicinal plant used as folk medicine, was investigated for its inhibition effects on carbonic anhydrase I and II enzymes. Four different extracts were obtained with maceration method and three different solvents were used. The results have shown that methanol extracts of Satureja cuneifolia has the strongest inhibition activity on the enzymes with the IC50 values of 31 µg/mL for hCA I and 12 µg/mL for hCA II. Further purification and analytical studies will be needed to obtain the active natural molecules and their potential for pharma and food industries.

Enzymes Inhibition and Antioxidant Potential of Medicinal Plants Growing in Oman

“…The H. lippii fractions also displayed appreciable potential except for the DCM and MeOH extracts, while aqueous extract was most potent presented IC 50 = 9:9 ± 0:35 μg/mL, proceeded by the nhexane with IC 50 = 18:8 ± 3:13 μg/mL and IC 50 = 35:6 ± 1:32 μg/mL in comparison to the standard acetazolamide having an IC 50 = 4:04 ± 1:63 μg/mL. The current results also match up with the outcomes of Aydin et al [69] for Satureja cuneifolia and dodoneine by Carreyre et al [70] which was found effective for the carbonic anhydrase activity. However, the study reported for the bioactive ingredient dodoneine by Carreyre et al [70] was significant as compared to our current findings because might be bioactive compounds are responsible compounds as compared to our selected plants and tested fractions…”