Snake venom disintegrins update: insights about new findings

Almeida, Gabriela de Oliveira; Oliveira, Isadora Sousa de; Arantes, Eliane Candiani; Sampaio, Suely Vilela

doi:10.1590/1678-9199-jvatitd-2023-0039

Cited by 7 publications

(7 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since D. russelii venom contains disintegrins and disintegrin-like proteins 30 , they may impact the migration of myoblasts. These proteins are rarely found in elapid venoms 31 and therefore, these venoms may not adversely affect integrins-mediated cell migration. Previous studies have reported that an SVMP from B. alternatus reduces the migration of myoblasts in culture through mechanisms dependent and independent of its protease activity 32 .…”

Section: Discussionmentioning

confidence: 99%

Differential effects of the venoms of Russell’s viper and Indian cobra on human myoblasts

Bin Haidar,

Almeida,

Williams

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Local tissue damage following snakebite envenoming remains a poorly researched area. To develop better strategies to treat snakebites, it is critical to understand the mechanisms through which venom toxins induce envenomation effects including local tissue damage. Here, we demonstrate how the venoms of two medically important Indian snakes (Russell's viper and cobra) affect human skeletal muscle using a cultured human myoblast cell line. The data suggest that both venoms affect the viability of myoblasts. Russell’s viper venom reduced the total number of cells, their migration, and the area of focal adhesions. It also suppressed myogenic differentiation and induced muscle atrophy. While cobra venom decreased the viability, it did not largely affect cell migration and focal adhesions. Cobra venom affected the formation of myotubes and induced atrophy. Cobra venom-induced atrophy could not be reversed by small molecule inhibitors such as varespladib (a phospholipase A2 inhibitor) and prinomastat (a metalloprotease inhibitor), and soluble activin type IIb receptor (a molecule used to promote regeneration of skeletal muscle), although the antivenom (raised against the Indian ‘Big Four’ snakes) has attenuated the effects. However, all these molecules rescued the myotubes from Russell’s viper venom-induced atrophy. This study demonstrates key steps in the muscle regeneration process that are affected by both Indian Russell’s viper and cobra venoms and offers insights into the potential causes of clinical features displayed in envenomed victims. Further research is required to investigate the molecular mechanisms of venom-induced myotoxicity under in vivo settings and develop better therapies for snakebite-induced muscle damage.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential effects of the venoms of Russell’s viper and Indian cobra on human myoblasts

Bin Haidar,

Almeida,

Williams

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In addition, this compound undergoes a chemical change that increases its affinity for platelet glycoproteins, especially GPIIb/IIIa receptors [ 32 ]. Therefore, this drug is able to prevent platelet aggregation and other thrombotic activities by competing with fibrinogen for the RGD domain recognition site in the GPIIb/IIIa receptor [ 98 , 99 , 100 ]. In 1998, the Food and Drug Administration (FDA) approved tirofiban as a therapeutic intervention for acute coronary syndrome [ 101 ].…”

Section: Utilization Of Svd As Pro-angiogenic Agentsmentioning

confidence: 99%

The Role of Snake Venom Disintegrins in Angiogenesis

Clissa,

Della-Casa,

Zychar

et al. 2024

Toxins

View full text Add to dashboard Cite

Angiogenesis, the formation of new blood vessels, plays a critical role in various physiological and pathological conditions. Snake venom disintegrins (SVDs) have been identified as significant regulators of this process. In this review, we explore the dual roles of SVD in angiogenesis, both as antiangiogenic agents by inhibiting integrin binding and interfering with vascular endothelial growth factors and as proangiogenic agents by enhancing integrin binding, stimulating cell migration and proliferation, and inducing neoangiogenesis. Studies in vitro and in animal models have demonstrated these effects and offer significant therapeutic opportunities. The potential applications of SVD in diseases related to angiogenesis, such as cancer, ocular diseases, tissue regeneration, wound healing, and cardiovascular diseases, are also discussed. Overall, SVDs are promising potential therapeutics, and further advances in this field could lead to innovative treatments for diseases related to angiogenesis.

show abstract

“…They are potent inhibitors of both platelet aggregation and integrin-dependent cell adhesion. Some SVMPs contain a disintegrin domain [41,42].…”

Section: Snake Venom Metalloproteinases (Svmps)mentioning

confidence: 99%

Dabsylated Bradykinin Is Cleaved by Snake Venom Proteases from Echis ocellatus

Abiola,

Berg,

Aiyelaagbe

et al. 2024

Biomedicines

View full text Add to dashboard Cite

The vasoactive peptide bradykinin (BK) is an important member of the renin–angiotensin system. Its discovery is tightly interwoven with snake venom research, because it was first detected in plasma following the addition of viper venom. While the fact that venoms liberate BK from a serum globulin fraction is well described, its destruction by the venom has largely gone unnoticed. Here, BK was found to be cleaved by snake venom metalloproteinases in the venom of Echis ocellatus, one of the deadliest snakes, which degraded its dabsylated form (DBK) in a few minutes after Pro7 (RPPGFSP↓FR). This is a common cleavage site for several mammalian proteases such as ACE, but is not typical for matrix metalloproteinases. Residual protease activity < 5% after addition of EDTA indicated that DBK is also cleaved by serine proteases to a minor extent. Mass spectrometry-based protein analysis provided spectral proof for several peptides of zinc metalloproteinase-disintegrin-like Eoc1, disintegrin EO4A, and three serine proteases in the venom.

show abstract

Snake venom disintegrins update: insights about new findings

Cited by 7 publications

References 142 publications

Differential effects of the venoms of Russell’s viper and Indian cobra on human myoblasts

Differential effects of the venoms of Russell’s viper and Indian cobra on human myoblasts

The Role of Snake Venom Disintegrins in Angiogenesis

Dabsylated Bradykinin Is Cleaved by Snake Venom Proteases from Echis ocellatus

Contact Info

Product

Resources

About