Differential expression of stem cell-like proteins in normal, hyperplastic and dysplastic oral epithelium

Barakat, Sarah Mohammed Mohammed; Siar, Chong Huat

doi:10.1590/1678-775720140245

Cited by 11 publications

(17 citation statements)

References 30 publications

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“…In the current study, we verified that groups with OL lesions (low‐ and high‐risk) showed a greater expression of SOX‐2 in the suprabasal layer, suggesting an expansion of the stem cell‐like population from the basal layer to the suprabasal layers in the oral dysplastic epithelium, as observed for the expression of β1 integrin and podoplanin (Barakat & Siar, ; Habiba et al., ; Kawaguchi et al., ).…”

Section: Discussionsupporting

confidence: 79%

“…This same rectangle was used for all evaluated images. A “hot spot” region of SOX‐2 in the epithelium, delimited by this rectangle, was evaluated in each case at a magnification of 100X (Barakat & Siar, ). In these regions, all positive and negative epithelial cells were counted (using the “events tool”), stratified by layers (basal and suprabasal).…”

Section: Methodsmentioning

confidence: 99%

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Immunoexpression of SOX‐2 in oral leukoplakia

et al. 2018

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Immunoexpression of SOX‐2 in oral leukoplakia

et al. 2018

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“…Cks were widely studied in the field of OSCC and OPMD. Ck19 is eventually found in the basal layer of the normal oral epithelium of non-keratinized mucosa, but overexpressed in OSCC ( 15 ), OPMD, and epithelial dysplasia ( 16 ). Ck19 was also proposed as a nonspecific Oral Keratinocytes Stem Cells (OKSC) marker ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

Clinical and Immunohistochemical epithelial profile of non-healing chronic traumatic ulcers

Gilligan¹,

Panico²,

Tada³

et al. 2020

Med Oral

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Background Chronic wounds were previously related to cancer. Chronic Traumatic Ulcers (CTU) are lesions caused by chronic mechanical irritation (CMI) frequently diagnosed in Oral Medicine. Although these conditions may reflect a benign nature, some authors have proposed its relationship with malignant transformation. Currently, there are scarce investigations that evaluate biomarkers within CTU. The aim of this study was to evaluate cell differentiation and proliferation biomarkers patterns of CTU and OSCC through recognized markers such as cytokeratin 19 and Ki67 and correlate it with clinical features of both groups of patients. Material and Methods A Cross-sectional study of adult patients (n=79), both sexes, attended at Oral Medicine Department, Facultad de Odontología, Universidad Nacional de Córdoba. The patients were classified into two groups: CTU (n=41), and OSCC (n=38). A subset of specimens were immunolabeled with Ki67 and Ck19. Results The population consisted of 51.9% male and 48.1% female, with an average of 57.0 ± 13.9. years (OSCC group) and 60.9 ± 14.9 years (CTU group). OSCC group presented higher scores for both biomarkers (Ki67 and Ck19), but only there were differences statistically significant for Ki67 ( p =0.032). 25% of non-healing CTU were positive with medium scores of Ck19 and showed an immunohistochemical profile similar to OSCC. The lateral tongue was the most frequent site in both groups. Conclusions The altered immunohistochemical pattern found in many specimens of CTU was also observed in OSCC. The tongue border presents physiological conditions that could offer a suitable environment for the development of neoplastic events associated with CMI. Further studies are needed to understand the underlying mechanisms that could link oral non-healing ulcers with early malignant changes. Key words: Ck19, Ki67, Oral Cancer, Chronic Traumatic Ulcer, Chronic Mechanical Irritation.

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“…These cells may function as a cell of origin in intestinal cancer (30). Furthermore, K15 was predominantly detected in the basal cell zone of the normal oral mucosa (31). However, other studies have shown that K15 is not always restricted to the basal layers of the epithelium, but it has a variant distribution in different normal tissues.…”

Section: Discussionmentioning

confidence: 98%

Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

2019

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The etiology and pathogenesis of odontogenic lesions are poorly understood. Keratin 15 (K15) is a type I cytoskeletal protein that provides structural support to the cells and has been considered to be a stem cell marker. The aim of the present study was to evaluate the expression of K15 in the epithelial lining of dentigerous cysts (DCs), odontogenic keratocysts (OKCs) and ameloblastomas (ABs). The study included 41 samples of DCs (n=13), OKCs (n=12), and AB tissues (n=16). K15 protein expression was evaluated via immunohistochemistry and data were statistically analyzed using a Kruskal-Wallis test. K15 was expressed in the majority of the studied lesions with various distributions in the different study samples. The Kruskal-Wallis test revealed non-significant differences in the expression of K15 among the three odontogenic lesions (P=0.380). The present study confirmed the high expression of K15 in the different epithelial layers of DC, OKC and AB. This type of expression excludes the reliability of regarding K15 as a stem cell marker in DC, OKC and AB. However, K15 may reflect the abnormal differentiation of pathological epithelial cells in these lesions.

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Differential expression of stem cell-like proteins in normal, hyperplastic and dysplastic oral epithelium

Cited by 11 publications

References 30 publications

Immunoexpression of SOX‐2 in oral leukoplakia

Immunoexpression of SOX‐2 in oral leukoplakia

Clinical and Immunohistochemical epithelial profile of non-healing chronic traumatic ulcers

Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

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