Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

Alsaegh, Mohammed Amjed; Altaie, Alaa Muayad; Zhu, Shu

doi:10.3892/mco.2019.1802

Cited by 6 publications

(11 citation statements)

References 38 publications

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“…A series of studies have shown that rodent incisors grow continuously throughout life owing to the epithelial progenitor cells in the cervical loop, an area where the inner and the outer enamel epithelium meet at the rim of the enamel organ at bell stage (12)(13)(14). Moreover, stem cell markers such as Oct-4, CD44 and K15 have been demonstrated in odontogenic lesions (15,16). Evidences above suggest the possibility that there are stem cells in the capsule of DC.…”

Section: Introductionmentioning

confidence: 99%

Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

Zhou

et al. 2020

Preprint

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Background: Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables bone to regenerate with capsules maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC derive from odontogenic epithelium remnants at embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization.Methods: Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues were analyzed by morphology, surface marker and multi-differentiation assays. Comparison of proliferation ability between Am-DCSCs and Bm-DCSCs were evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F) and 5’‐ethynyl‐2’‐deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by Alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with Real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice were performed to detect their bone regeneration and bone defect repair ability.Results: Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony forming cells expressed MSC markers (CD44+, CD90+, CD31-, CD34-, CD45-), and they could differentiate into osteoblast-, adipocyte- and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs. Conclusions: There are MSCs residing in capsules of DC, and the cell viability as well as osteogenic capacity of them are largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.

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Section: Introductionmentioning

confidence: 99%

Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

Zhou

et al. 2020

Preprint

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“…However, knowledge is little about the expression of K15 in odontogenic cysts and tumors. We have previously investigated the expression of K15 in dentigerous cyst, odontogenic keratocyst, and ameloblastoma ( 16 ). Moreover, the expression of COX-2 and K15 in RCs is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

The correlated expression of COX-2 and keratin 15 in radicular cysts

et al. 2022

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Background The expression of cyclooxygenase-2 (COX-2) and Keratin-15 (K15) in radicular cysts (RCs) is poorly understood. Identifying the expression of these two markers may modify the current treatment of RC. The objective of this study was to evaluate the expression of COX-2 and its relationship to K15 expression in the odontogenic epithelial cells of the RC. Material and Methods A total of 18 RCs were immunohistochemically analyzed for COX-2 and K15 expression. The cellular inflammatory reaction in the cyst wall was also assessed by measuring the percentage of inflammatory cells to the total number of cells. Results COX-2 expression in the odontogenic epithelium of RC was absent in 11.1 % (n=2), mild in 27.8 % (n=5), moderate in 22.2% (n=4) and strong in 38.9% (n=7). Meanwhile, K15 expression was absent in 27.8% (n=5), mild in 16.7% (n=3), moderate in 44.4% (n=8), and strong in 11.1% (n=2) of the cases. The inflammatory infiltrate was mild in 2 cases (11.1%), moderate in 6 cases (33.3%), and high in 10 cases (55.6%). Spearman’s correlation test revealed significant correlation (rho= .533; p = .023) between COX-2 and K15 expression in the odontogenic epithelium of RC. However, no correlation was noted between inflammation and expression of COX-2 (rho= 0.248, p =.321) or K15 (rho= -0.162, p = .520). Conclusions There is high and correlated expression of COX-2 and K15 in the odontogenic epithelium of RC. COX-2 could therefore be involved in epithelial cell differentiation of the cyst. Additionally, the expression of K15 in RC may be an indicator of epithelial cell differentiation. Key words: Cyclooxygenase, COX-2, Keratin-15, K15, Radicular cyst.

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“… 9 The expression of multiple cytokeratins in cysts and odontogenic tumors such as CK5/6, 7, 13, 14, 15, 16, 17, 19, AE1/AE3 has been reported. 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 CK14 is considered to be the main odontogenic epithelium cytokeratin. 7 Currently, the role of amelogenin in aggressiveness of OC and OT is controversial, since some studies find no difference in the expression of amelogenin in benign or malignant OT while other authors associate it with greater cell differentiation and less aggressiveness.…”

Section: Introductionmentioning

confidence: 99%

Amelogenin in calcified matrices of odontogenic cysts and odontogenic tumors: An immunohistochemical study

Urzúa

Ahumada-Ossandón

Casa-Weisser

et al. 2021

Journal of Dental Sciences

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Background/purpose There are few studies comparing the expression of enamel proteins, such as amelogenin, and cytokeratins in cyst and odontogenic tumors like in ameloblastoma and odontogenic keratocyst, indicating that amelogenin could be a potential biomarker for the aggressiveness in the odontogenic tumors. The aim of this study was to evaluate if the expression of amelogenin, cytokeratin AE1/AE3 (CKAE1/AE3) and cytokeratin 14 (CK14) in cysts and odontogenic tumors with calcified matrices such as calcifying odontogenic cyst (COC), compound (CdO) and complex (CxO) odontomas, adenomatoid odontogenic tumor (AOT) and calcifying epithelial odontogenic tumor (CEOT) as an aggressiveness indicator. Materials and methods Three COC, eight CxO, three CdO, twelve AOT, two CEOT and three dental germs were submitted to an immunohistochemistry panel of antibodies composed of amelogenin, CKAE1/AE3 and CK14. Results CKAE1/AE3 and CK14 was present in all odontogenic epithelia. The amelogenin protein was detected in prismatic and amorphous calcified matrices of epithelial origin belonging to CxO, CdO, AOT, COC and the tooth germs used as controls. On the other hand, the CEOT was the only tumor or cyst studied that did not present immunostaining for amelogenin in calcified matrices. Conclusion Amelogenin was detected in pathologies with a low or absent recurrence rate and excellent prognosis. CEOT was the lesion of greater clinical aggressiveness which did not express amelogenin. The presence of amelogenin in calcified matrices of odontogenic arise could be an indicator of low aggressiveness.

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Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma

Cited by 6 publications

References 38 publications

Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

The correlated expression of COX-2 and keratin 15 in radicular cysts

Amelogenin in calcified matrices of odontogenic cysts and odontogenic tumors: An immunohistochemical study

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