Overexpression of S100A4 as a biomarker of metastasis and recurrence in oral squamous cell carcinoma

Natarajan, Jayalakshmi; Hunter, Keith D.; Mutalik, Vimi Sunil; Radhakrishnan, Raghu

doi:10.1590/1678-775720140133

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…Interestingly, S100A8/A9 were found to be up-regulated in cutaneous squamous cell carcinoma compared to premalignant skin lesions (49). Enhanced S100A4 expression in combination with a decreased expression of S100A8/A9 in OSCCs indicates a potentially aggressive behavior of OSCCs (27,28,32,39).…”

Section: Discussionmentioning

confidence: 98%

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Cellular Distribution and Gene Expression Pattern of Metastasin (S100A4), Calgranulin A (S100A8), and Calgranulin B (S100A9) in Oral Lesions as Markers for Molecular Pathology

Reckenbeil

Kraus

Probstmeier

et al. 2016

Cancer Investigation

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The objective of this study was to analyze cellular localization and expression levels of oncologic relevant members of the S100 family in common oral lesions.Biopsies of various oral lesions were analyzed. S100A4 showed a higher expression rate in leukoplakias and oral squamous cell carcinomas. Transcript levels of S100A8 and S100A9 were significantly decreased in malignant OSCCs. A correlation could be drawn between the expression levels of these genes and the pathological characteristics of the investigated lesions. S100A4, A8, and A9 proteins represent promising marker genes to evaluate the risk potential of suspicious oral lesions in molecular pathology.

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Section: Discussionmentioning

confidence: 98%

“…A high level of S100A4 protein promotes a migratory phenotype, epithelialmesenchymal transition (EMT) and is therefore conducive to metastasis formation (23,(28)(29)(30)(31). In oral squamous cell carcinoma, S100A4 was also found to be involved and positively correlated with metastasis (32,33).…”

Section: Introductionmentioning

confidence: 99%

Cellular Distribution and Gene Expression Pattern of Metastasin (S100A4), Calgranulin A (S100A8), and Calgranulin B (S100A9) in Oral Lesions as Markers for Molecular Pathology

Reckenbeil

Kraus

Probstmeier

et al. 2016

Cancer Investigation

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“…FAM107B protein shares 65% sequence similarity with FAM107A, the other member of FAM107, in their N-terminal DUF1151 regions (Nakajima et al, 2010). The increased S100A4 expression was closely correlated with invasion, lymph node metastasis, and poor prognosis of gastric cancer (Li et al, 2013;Natarajan et al, 2014;Niu et al, 2015). Functional analysis in vitro showed that forced expression of HITS protein suppresses the responses of Hela cells to growth factors (Nakajima et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…S100A4 can influence various biological characteristics of several different kinds of cancer cells (Cheng et al, 2016;Qi et al, 2016;Zhang et al, 2016). The increased S100A4 expression was closely correlated with invasion, lymph node metastasis, and poor prognosis of gastric cancer (Li et al, 2013;Natarajan et al, 2014;Niu et al, 2015). Recently, we analyzed the differentially expressed gene profile after S100A4 inhibition in gastric cancer cells MGC803 by cDNA microarray and identified 179 differentially expressed genes (Guo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

FAM107B is regulated by S100A4 and mediates the effect of S100A4 on the proliferation and migration of MGC803 gastric cancer cells

Guo

Bian

Wang

et al. 2017

Cell Biology International

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FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression of FAM107B in cancers are not clear, the functional significance needs to be further clarified. Our previous findings from cDNA microarray showed that there are 179 differentially expressed genes after S100A4 inhibition in gastric cancer cells MGC803. FAM107B was an upregulated one among them. In the present study, we confirmed that FAM107B expression was upregulated in MGC803 cells after S100A4 inhibition by qRT-PCR. We demonstrated for the first time that FAM107B was downregulated by S100A4. The results from CCK-8 and transwell assay showed that FAM107B inhibition by siRNA led to significantly increased proliferation and migrating abilities of MGC803 cells, respectively, indicating that FAM107B plays important roles in inhibiting the proliferation and migration of MGC803 cells. The rescue experiment showed that FAM107B-siRNA transfection reversed the reduced proliferation and migration abilities induced by S100A4 inhibition in the cells. These findings suggest that, as a downstream effector, FAM107B at least partly mediates the effect of S100A4 on the proliferation and migration of MGC803 cells. In conclusion, we first provide experimental evidence suggesting that FAM107B was downregulated by S100A4 in gastric cancer MGC803 cells. And FAM107B at least partially mediates the biological effect of S100A4 in the cells.

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“…The S100A4 protein occurs as noncovalently bound homodimers with the ability to interact with an array of target proteins in a calcium-dependent manner (1). S100A4 overexpression has been reported in several types of cancer and is associated with invasion and metastasis and poor patient prognosis (2)(3)(4). Many studies have confirmed that S100A4 is involved in a variety of biological effects.…”

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confidence: 99%

S100A4 influences cancer stem cell-like properties of MGC803 gastric cancer cells by regulating GDF15 expression

Guo

Bian

Wang

et al. 2016

International Journal of Oncology

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Many studies have revealed that S100A4 is involved in cancer progression by affecting a variety of biological functions. Our previous study showed that S100A4 influences many biological properties of gastric cancer cells; however, the underlying mechanisms are far from clear. In this study, we used cDNA microarray analysis to investigate the global alterations in gene expression in MGC803 gastric cancer cells after siRNA-mediated S100A4 inhibition. Among the total genes investigated, 179 differentially expressed genes (38 upregulated and 141 downregulated) were detected in S100A4-siRNA transfected MGC803 cells compared with NC-siRNA transfected cells. We focused on the GDF15 gene, which was significantly downregulated after S100A4 inhibition. ChIP studies showed that the S100A4 protein binds to the GDF15 promoter, implicating S100A4 in GDF15 regulation at the transcriptional level. GDF15 overexpression promoted CSC-like properties of MGC803 cells, such as spheroid and soft-agar colony forming abilities. S100A4 inhibition suppressed the CSC-like properties of the cells, whereas, GV141-GDF15 vector transfection reversed these effects. Our results suggest that S100A4 influences the CSC-like properties of MGC803 gastric cancer cells by regulating GDF15 expression.

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Overexpression of S100A4 as a biomarker of metastasis and recurrence in oral squamous cell carcinoma

Cited by 15 publications

References 27 publications

Cellular Distribution and Gene Expression Pattern of Metastasin (S100A4), Calgranulin A (S100A8), and Calgranulin B (S100A9) in Oral Lesions as Markers for Molecular Pathology

Cellular Distribution and Gene Expression Pattern of Metastasin (S100A4), Calgranulin A (S100A8), and Calgranulin B (S100A9) in Oral Lesions as Markers for Molecular Pathology

FAM107B is regulated by S100A4 and mediates the effect of S100A4 on the proliferation and migration of MGC803 gastric cancer cells

S100A4 influences cancer stem cell-like properties of MGC803 gastric cancer cells by regulating GDF15 expression

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